Lista de obras -

'Dual' peptidyl-oligonucleotide conjugates: Role of conformational flexibility in catalytic cleavage of RNA.

artículo científico publicado en 2016

2'-O-methyl-modified anti-MDR1 fork-siRNA duplexes exhibiting high nuclease resistance and prolonged silencing activity

artículo científico publicado en 2010

42- and 63-bp anti-MDR1-siRNAs bearing 2'-OMe modifications in nuclease-sensitive sites induce specific and potent gene silencing

artículo científico publicado en 2014

82 Structural aspects of non-enzymatic recombination in viral RNA.

artículo científico publicado en 2015

A novel expression cassette delivers efficient production of exclusively tetrameric human butyrylcholinesterase with improved pharmacokinetics for protection against organophosphate poisoning.

artículo científico publicado en 2015

Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with alkylating groups at the 3'-end

artículo científico publicado el 1 de julio de 1992

Alteration of the exDNA profile in blood serum of LLC-bearing mice under the decrease of tumour invasion potential by bovine pancreatic DNase I treatment

artículo científico publicado en 2017

Animal model of drug-resistant tumor progression

artículo científico publicado en 2006

Antitumor and Antimetastatic Effect of Small Immunostimulatory RNA against B16 Melanoma in Mice

artículo científico publicado en 2016

Antitumour Activity of the Ribonuclease Binase from Bacillus pumilus in the RLS40 Tumour Model Is Associated with the Reorganisation of the miRNA Network and Reversion of Cancer-Related Cascades to Normal Functioning

artículo científico publicado en 2020

Artificial ribonucleases inactivate a wide range of viruses using their ribonuclease, membranolytic, and chaotropic-like activities.

artículo científico publicado en 2016

Asthma and Post-Asthmatic Fibrosis: A Search for New Promising Molecular Markers of Transition from Acute Inflammation to Pulmonary Fibrosis

artículo científico publicado en 2022

Autocrine-based selection of ligands for personalized CAR-T therapy of lymphoma

artículo científico publicado en 2018

Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group

artículo científico publicado en 2011

Catalytic Knockdown of miR-21 by Artificial Ribonuclease: Biological Performance in Tumor Model

scientific article published on 08 August 2019

Chemical modification of siRNA.

artículo científico publicado en 2010

Chemical ribonucleases

artículo científico publicado el 1 de enero de 1998

Cholesterol-Containing Nuclease-Resistant siRNA Accumulates in Tumors in a Carrier-free Mode and Silences MDR1 Gene.

artículo científico publicado en 2017

Comparison of behaviour in different liquids and in cells of gold nanorods and spherical nanoparticles modified by linear polyethyleneimine and bovine serum albumin

artículo científico publicado en 2014

Correction to Peptidyl-Oligonucleotide Conjugates Demonstrate Efficient Cleavage of RNA in a Sequence-Specific Manner

artículo científico publicado en 2015

Covalently attached oligodeoxyribonucleotides induce RNase activity of a short peptide and modulate its base specificity.

artículo científico publicado en 2004

Cyano Enone-Bearing Triterpenoid Soloxolone Methyl Inhibits Epithelial-Mesenchymal Transition of Human Lung Adenocarcinoma Cells In Vitro and Metastasis of Murine Melanoma In Vivo

artículo científico publicado en 2020

Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer

scientific article published on 01 November 2019

Deep insights into the response of human cervical carcinoma cells to a new cyano enone-bearing triterpenoid soloxolone methyl: a transcriptome analysis

scientific article published on 03 September 2019

Design of nuclease-resistant fork-like small interfering RNA (fsiRNA).

artículo científico publicado en 2013

Design, RNA cleavage and antiviral activity of new artificial ribonucleases derived from mono-, di- and tripeptides connected by linkers of different hydrophobicity

artículo científico publicado en 2016

Design, synthesis and transfection efficiency of a novel redox-sensitive polycationic amphiphile

artículo científico publicado en 2016

Downregulation of PGY1/MDR1 mRNA level in human KB cells by antisense oligonucleotide conjugates. RNA accessibility in vitro and intracellular antisense activity.

artículo científico publicado en 2002

Dual Effect of Soloxolone Methyl on LPS-Induced Inflammation In Vitro and In Vivo

artículo científico publicado en 2020

Dual miRNases for Triple Incision of miRNA Target: Design Concept and Catalytic Performance

artículo científico publicado en 2020

Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3'-modified oligo(2'-O-methylribonucleotide)-effectors.

artículo científico publicado en 2008

Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease

artículo científico publicado en 2005

Enhanced cellular binding of concatemeric oligonucleotide complexes

artículo científico

Erratum to: Oncolytic virus efficiency inhibited growth of tumour cells with multiple drug resistant phenotype in vivo and in vitro.

artículo científico publicado en 2016

Female scent signals enhance the resistance of male mice to influenza

artículo científico publicado en 2010

Fluorophore Labeling Affects the Cellular Accumulation and Gene Silencing Activity of Cholesterol-Modified siRNAs In Vitro

scientific article published on 18 December 2018

Functional topography of human ribosomes as studied by affinity labeling with reactive mRNA analogs ⬇️

artículo científico publicado el 1 de abril de 1992

G-specific RNA-cleaving conjugates of short peptides and oligodeoxyribonucleotides.

artículo científico publicado en 2006

Human Recombinant DNase I (Pulmozyme®) Inhibits Lung Metastases in Murine Metastatic B16 Melanoma Model That Correlates with Restoration of the DNase Activity and the Decrease SINE/LINE and c-Myc Fragments in Blood Cell-Free DNA

artículo científico publicado en 2021

Hybridization of antisense oligonucleotides with alpha-sarcin loop region of Escherichia coli 23S rRNA.

artículo científico publicado en 2004

Identification of Novel Core Genes Involved in Malignant Transformation of Inflamed Colon Tissue Using a Computational Biology Approach and Verification in Murine Models

scientific article published in 2023

Immunostimulating RNA Delivered by P1500 PEGylated Cationic Liposomes Limits Influenza Infection in C57Bl/6 Mice

scientific article published on 14 September 2020

Immunotherapy of hepatocellular carcinoma with small double-stranded RNA.

artículo científico publicado en 2014

Inactivation of a non-enveloped RNA virus by artificial ribonucleases: honey bees and acute bee paralysis virus as a new experimental model for in vivo antiviral activity assessment.

artículo científico publicado en 2011

Inactivation of the tick-borne encephalitis virus by RNA-cleaving compounds

artículo científico publicado en 2011

Influenza virus inactivated by artificial ribonucleases as a prospective killed virus vaccine

artículo científico publicado en 2012

Inhibition of metastasis development by daily administration of ultralow doses of RNase A and DNase I.

artículo científico publicado en 2010

Isolation of ribosomal subunits containing intact rRNA from human placenta: Estimation of functional activity of 80S ribosomes ⬇️

artículo científico publicado el 1 de noviembre de 1991

Mechanism of Antisense Oligonucleotide Interaction with Natural RNAs ⬇️

artículo científico publicado el 1 de agosto de 2011

Mechanism of cytotoxicity of artificial ribonucleases in various human cancer cell lines

artículo científico publicado el 1 de marzo de 2010

Mesyl phosphoramidate backbone modified antisense oligonucleotides targeting miR-21 with enhanced in vivo therapeutic potency

scientific article published on 07 December 2020

MicroRNA drop in the bloodstream and microRNA boost in the tumour caused by treatment with ribonuclease A leads to an attenuation of tumour malignancy

artículo científico publicado en 2013

Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells

artículo científico publicado en 2016

Modulation of Tumour-Related Signaling Pathways by Natural Pentacyclic Triterpenoids and their Semisynthetic Derivatives

artículo científico publicado en 2017

Molecular Mechanism of the Antiproliferative Activity of Short Immunostimulating dsRNA

artículo científico publicado en 2019

Morphologic Changes in the Tumor and Liver in Mice with Transplanted RLS40 Lymphosarcoma during Increase of Its Drug Resistance ⬇️

artículo científico publicado el 1 de agosto de 2010

Multicomponent mannose-containing liposomes efficiently deliver RNA in murine immature dendritic cells and provide productive anti-tumour response in murine melanoma model

artículo científico publicado en 2015

Non-enzymatic recombination of RNA: Ligation in loops.

artículo científico publicado en 2017

Non-enzymatic template-directed recombination of RNAs.

artículo científico publicado en 2009

Nonenzymatic recombination of RNA: possible mechanism for the formation of novel sequences

artículo científico publicado en 2007

Novel 3'-Substituted-1',2',4'-Oxadiazole Derivatives of 18βH-Glycyrrhetinic Acid and Their O-Acylated Amidoximes: Synthesis and Evaluation of Antitumor and Anti-Inflammatory Potential In Vitro and In Vivo ⬇️

artículo científico

Novel 5'-Norcarbocyclic Derivatives of Bicyclic Pyrrolo- and Furano[2,3-d]Pyrimidine Nucleosides

scientific article published on 16 October 2018

Novel Derivatives of Deoxycholic Acid Bearing Linear Aliphatic Diamine and Aminoalcohol Moieties and their Cyclic Analogs at the C3 Position: Synthesis and Evaluation of Their In Vitro Antitumor Potential

artículo científico publicado en 2019

Novel PEGylated Liposomes Enhance Immunostimulating Activity of isRNA

scientific article published on 27 November 2018

Novel cationic liposomes provide highly efficient delivery of DNA and RNA into dendritic cell progenitors and their immature offsets

artículo científico publicado en 2011

Novel cholesterol spermine conjugates provide efficient cellular delivery of plasmid DNA and small interfering RNA.

artículo científico publicado en 2011

Novel cholesterol-based cationic lipids for gene delivery.

artículo científico publicado en 2009

Novel derivatives of deoxycholic acid bearing aliphatic or cyclic diamine moieties at the C-3 position: Synthesis and evaluation of anti-proliferative activity

artículo científico publicado en 2017

Novel multifunctional hybrids of single-walled carbon nanotubes with nucleic acids: synthesis and interactions with living cells

artículo científico publicado en 2014

Nuclease-resistant 63-bp trimeric siRNAs simultaneously silence three different genes in tumor cells.

artículo científico publicado en 2017

Oncolytic virus efficiency inhibited growth of tumour cells with multiple drug resistant phenotype in vivo and in vitro

artículo científico publicado en 2016

Peptidyl-oligonucleotide conjugates demonstrate efficient cleavage of RNA in a sequence-specific manner

artículo científico publicado en 2015

Prophylactic Dendritic Cell-Based Vaccines Efficiently Inhibit Metastases in Murine Metastatic Melanoma

artículo científico publicado en 2015

RNA knockdown by synthetic peptidyl-oligonucleotide ribonucleases: behavior of recognition and cleavage elements under physiological conditions

artículo científico publicado en 2020

RNA-hydrolyzing activity of human serum albumin and its recombinant analogue.

artículo científico publicado en 2010

Ribonuclease binase inhibits primary tumor growth and metastases via apoptosis induction in tumor cells

artículo científico publicado en 2013

Selective protection of nuclease-sensitive sites in siRNA prolongs silencing effect

artículo científico publicado en 2009

Selectivity of the sequence specific alkylation of high molecular weight RNA ⬇️

artículo científico publicado el 1 de enero de 1991

Sequence-specific artificial ribonucleases. I. Bis-imidazole-containing oligonucleotide conjugates prepared using precursor-based strategy

artículo científico publicado en 2004

Short double-stranded RNA with immunostimulatory activity: sequence dependence

artículo científico publicado en 2012

Silencing activity of 2′-O-methyl modified anti-MDR1 siRNAs with mismatches in the central part of the duplexes

artículo científico publicado en 2011

Silencing of Her2, CCNB1 and PKC Genes by siRNA Results in Prolonged Retardation of Neuroblastoma Cell Division ⬇️

artículo científico publicado el 1 de julio de 2011

Silencing of Inducible Immunoproteasome Subunit Expression by Chemically Modified siRNA and shRNA.

artículo científico publicado en 2016

Site-selective artificial ribonucleases: oligonucleotide conjugates containing multiple imidazole residues in the catalytic domain.

artículo científico

Soloxolone methyl inhibits influenza virus replication and reduces virus-induced lung inflammation

artículo científico publicado en 2017

Spacer structure and hydrophobicity influences transfection activity of novel polycationic gemini amphiphiles.

artículo científico publicado en 2017

Specifically activated dendritic cells—Cell-based vaccine against lymphosarcoma exhibiting multiple drug resistance phenotype ⬇️

artículo científico publicado el 1 de septiembre de 2009

Strict conformational demands of RNA cleavage in bulge-loops created by peptidyl-oligonucleotide conjugates

scientific article published on 03 October 2020

Structure-transfection activity relationships in a series of novel cationic lipids with heterocyclic head-groups

artículo científico publicado en 2013

Synthesis and biological activity of novel deoxycholic acid derivatives

artículo científico publicado en 2015

Synthesis and delivery activity of new cationic cholesteryl glucosides.

artículo científico publicado en 2010

Synthesis and evaluation of antitumor, anti-inflammatory and analgesic activity of novel deoxycholic acid derivatives bearing aryl- or hetarylsulfanyl moieties at the C-3 position

artículo científico publicado en 2017

Synthesis and pro-apoptotic activity of novel glycyrrhetinic acid derivatives.

artículo científico publicado en 2011

Synthesis of novel 2-cyano substituted glycyrrhetinic acid derivatives as inhibitors of cancer cells growth and NO production in LPS-activated J-774 cells.

artículo científico publicado en 2013

Targeted delivery of nucleic acids into xenograft tumors mediated by novel folate-equipped liposomes

artículo científico publicado en 2017

Targeting Circulating SINEs and LINEs with DNase I Provides Metastases Inhibition in Experimental Tumor Models

scientific article published on 08 February 2020

The Toxic Effects of Polychemotherapy onto the Liver Are Accelerated by the Upregulated MDR of Lymphosarcoma.

artículo científico publicado en 2012

The siRNA targeted to mdr1b and mdr1a mRNAs in vivo sensitizes murine lymphosarcoma to chemotherapy

artículo científico publicado en 2010

The systemic tumor response to RNase A treatment affects the expression of genes involved in maintaining cell malignancy

artículo científico publicado en 2017

Transfection efficiency of 25-kDa PEI-cholesterol conjugates with different levels of modification

artículo científico publicado en 2009

Transport Oligonucleotides-A Novel System for Intracellular Delivery of Antisense Therapeutics

artículo científico publicado en 2020

Trioxolone Methyl, a Novel Cyano Enone-Bearing 18βH-Glycyrrhetinic Acid Derivative, Ameliorates Dextran Sulphate Sodium-Induced Colitis in Mice

artículo científico publicado en 2020

Virtual screening, synthesis and biological evaluation of DNA intercalating antiviral agents

artículo científico publicado en 2017

[Complementary addressed alkylation of 16S rRNA of Escherichia coli by 2',3'-O-[4-N-methyl-N-(2-chloroethyl)-amino]benzylidene derivatives of oligodeoxyribonucleotides. V. Study of the factors affecting selectivity of modification]

artículo científico publicado el 1 de abril de 1991

miRNases: Novel peptide-oligonucleotide bioconjugates that silence miR-21 in lymphosarcoma cells.

artículo científico publicado en 2017

Lista de obras de

'Dual' peptidyl-oligonucleotide conjugates: Role of conformational flexibility in catalytic cleavage of RNA.

2'-O-methyl-modified anti-MDR1 fork-siRNA duplexes exhibiting high nuclease resistance and prolonged silencing activity

42- and 63-bp anti-MDR1-siRNAs bearing 2'-OMe modifications in nuclease-sensitive sites induce specific and potent gene silencing

82 Structural aspects of non-enzymatic recombination in viral RNA.

A novel expression cassette delivers efficient production of exclusively tetrameric human butyrylcholinesterase with improved pharmacokinetics for protection against organophosphate poisoning.

Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with alkylating groups at the 3'-end

Alteration of the exDNA profile in blood serum of LLC-bearing mice under the decrease of tumour invasion potential by bovine pancreatic DNase I treatment

Animal model of drug-resistant tumor progression

Antitumor and Antimetastatic Effect of Small Immunostimulatory RNA against B16 Melanoma in Mice

Antitumour Activity of the Ribonuclease Binase from Bacillus pumilus in the RLS40 Tumour Model Is Associated with the Reorganisation of the miRNA Network and Reversion of Cancer-Related Cascades to Normal Functioning

Artificial ribonucleases inactivate a wide range of viruses using their ribonuclease, membranolytic, and chaotropic-like activities.

Asthma and Post-Asthmatic Fibrosis: A Search for New Promising Molecular Markers of Transition from Acute Inflammation to Pulmonary Fibrosis

Autocrine-based selection of ligands for personalized CAR-T therapy of lymphoma

Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group

Catalytic Knockdown of miR-21 by Artificial Ribonuclease: Biological Performance in Tumor Model

Chemical modification of siRNA.

Chemical ribonucleases

Cholesterol-Containing Nuclease-Resistant siRNA Accumulates in Tumors in a Carrier-free Mode and Silences MDR1 Gene.

Comparison of behaviour in different liquids and in cells of gold nanorods and spherical nanoparticles modified by linear polyethyleneimine and bovine serum albumin

Correction to Peptidyl-Oligonucleotide Conjugates Demonstrate Efficient Cleavage of RNA in a Sequence-Specific Manner

Covalently attached oligodeoxyribonucleotides induce RNase activity of a short peptide and modulate its base specificity.

Cyano Enone-Bearing Triterpenoid Soloxolone Methyl Inhibits Epithelial-Mesenchymal Transition of Human Lung Adenocarcinoma Cells In Vitro and Metastasis of Murine Melanoma In Vivo

Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer

Deep insights into the response of human cervical carcinoma cells to a new cyano enone-bearing triterpenoid soloxolone methyl: a transcriptome analysis

Design of nuclease-resistant fork-like small interfering RNA (fsiRNA).

Design, RNA cleavage and antiviral activity of new artificial ribonucleases derived from mono-, di- and tripeptides connected by linkers of different hydrophobicity

Design, synthesis and transfection efficiency of a novel redox-sensitive polycationic amphiphile

Downregulation of PGY1/MDR1 mRNA level in human KB cells by antisense oligonucleotide conjugates. RNA accessibility in vitro and intracellular antisense activity.

Dual Effect of Soloxolone Methyl on LPS-Induced Inflammation In Vitro and In Vivo

Dual miRNases for Triple Incision of miRNA Target: Design Concept and Catalytic Performance

Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3'-modified oligo(2'-O-methylribonucleotide)-effectors.

Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease

Enhanced cellular binding of concatemeric oligonucleotide complexes

Erratum to: Oncolytic virus efficiency inhibited growth of tumour cells with multiple drug resistant phenotype in vivo and in vitro.

Female scent signals enhance the resistance of male mice to influenza

Fluorophore Labeling Affects the Cellular Accumulation and Gene Silencing Activity of Cholesterol-Modified siRNAs In Vitro

Functional topography of human ribosomes as studied by affinity labeling with reactive mRNA analogs ⬇️

G-specific RNA-cleaving conjugates of short peptides and oligodeoxyribonucleotides.

Human Recombinant DNase I (Pulmozyme®) Inhibits Lung Metastases in Murine Metastatic B16 Melanoma Model That Correlates with Restoration of the DNase Activity and the Decrease SINE/LINE and c-Myc Fragments in Blood Cell-Free DNA

Hybridization of antisense oligonucleotides with alpha-sarcin loop region of Escherichia coli 23S rRNA.

Identification of Novel Core Genes Involved in Malignant Transformation of Inflamed Colon Tissue Using a Computational Biology Approach and Verification in Murine Models

Immunostimulating RNA Delivered by P1500 PEGylated Cationic Liposomes Limits Influenza Infection in C57Bl/6 Mice

Immunotherapy of hepatocellular carcinoma with small double-stranded RNA.

Inactivation of a non-enveloped RNA virus by artificial ribonucleases: honey bees and acute bee paralysis virus as a new experimental model for in vivo antiviral activity assessment.

Inactivation of the tick-borne encephalitis virus by RNA-cleaving compounds

Influenza virus inactivated by artificial ribonucleases as a prospective killed virus vaccine

Inhibition of metastasis development by daily administration of ultralow doses of RNase A and DNase I.

Isolation of ribosomal subunits containing intact rRNA from human placenta: Estimation of functional activity of 80S ribosomes ⬇️

Mechanism of Antisense Oligonucleotide Interaction with Natural RNAs ⬇️

Mechanism of cytotoxicity of artificial ribonucleases in various human cancer cell lines

Mesyl phosphoramidate backbone modified antisense oligonucleotides targeting miR-21 with enhanced in vivo therapeutic potency

MicroRNA drop in the bloodstream and microRNA boost in the tumour caused by treatment with ribonuclease A leads to an attenuation of tumour malignancy

Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells

Modulation of Tumour-Related Signaling Pathways by Natural Pentacyclic Triterpenoids and their Semisynthetic Derivatives

Molecular Mechanism of the Antiproliferative Activity of Short Immunostimulating dsRNA

Morphologic Changes in the Tumor and Liver in Mice with Transplanted RLS40 Lymphosarcoma during Increase of Its Drug Resistance ⬇️

Multicomponent mannose-containing liposomes efficiently deliver RNA in murine immature dendritic cells and provide productive anti-tumour response in murine melanoma model

Non-enzymatic recombination of RNA: Ligation in loops.

Non-enzymatic template-directed recombination of RNAs.

Nonenzymatic recombination of RNA: possible mechanism for the formation of novel sequences

Novel 3'-Substituted-1',2',4'-Oxadiazole Derivatives of 18βH-Glycyrrhetinic Acid and Their O-Acylated Amidoximes: Synthesis and Evaluation of Antitumor and Anti-Inflammatory Potential In Vitro and In Vivo ⬇️

Novel 5'-Norcarbocyclic Derivatives of Bicyclic Pyrrolo- and Furano[2,3-d]Pyrimidine Nucleosides

Novel Derivatives of Deoxycholic Acid Bearing Linear Aliphatic Diamine and Aminoalcohol Moieties and their Cyclic Analogs at the C3 Position: Synthesis and Evaluation of Their In Vitro Antitumor Potential

Novel PEGylated Liposomes Enhance Immunostimulating Activity of isRNA

Novel cationic liposomes provide highly efficient delivery of DNA and RNA into dendritic cell progenitors and their immature offsets

Novel cholesterol spermine conjugates provide efficient cellular delivery of plasmid DNA and small interfering RNA.

Novel cholesterol-based cationic lipids for gene delivery.

Novel derivatives of deoxycholic acid bearing aliphatic or cyclic diamine moieties at the C-3 position: Synthesis and evaluation of anti-proliferative activity

Novel multifunctional hybrids of single-walled carbon nanotubes with nucleic acids: synthesis and interactions with living cells

Nuclease-resistant 63-bp trimeric siRNAs simultaneously silence three different genes in tumor cells.

Oncolytic virus efficiency inhibited growth of tumour cells with multiple drug resistant phenotype in vivo and in vitro

Peptidyl-oligonucleotide conjugates demonstrate efficient cleavage of RNA in a sequence-specific manner

Prophylactic Dendritic Cell-Based Vaccines Efficiently Inhibit Metastases in Murine Metastatic Melanoma

RNA knockdown by synthetic peptidyl-oligonucleotide ribonucleases: behavior of recognition and cleavage elements under physiological conditions

RNA-hydrolyzing activity of human serum albumin and its recombinant analogue.

Ribonuclease binase inhibits primary tumor growth and metastases via apoptosis induction in tumor cells

Selective protection of nuclease-sensitive sites in siRNA prolongs silencing effect

Selectivity of the sequence specific alkylation of high molecular weight RNA ⬇️

Sequence-specific artificial ribonucleases. I. Bis-imidazole-containing oligonucleotide conjugates prepared using precursor-based strategy