Lista de obras -

A benchmark for chromatin binding measurements in live cells.

artículo científico publicado en 2012

A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET).

artículo científico publicado en 2003

Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching.

artículo científico publicado en 2006

Analysis of binding reactions by fluorescence recovery after photobleaching

artículo científico publicado en 2004

Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy

Anti-angiogenic activity of human endostatin is HIF-1-independent in vitro and sensitive to timing of treatment in a human saphenous vein assay.

artículo científico publicado en 2003

Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches.

artículo científico publicado en 1998

Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription

artículo científico publicado en 2009

CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites

artículo científico publicado en 2013

Capping protein levels influence actin assembly and cell motility in dictyostelium

artículo científico publicado en 1995

Cells Undergo Major Changes in the Quantity of Cytoplasmic Organelles after Uptake of Gold Nanoparticles with Biologically Relevant Surface Coatings

scientific article published on 28 January 2020

Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks

artículo científico publicado en 2006

Changes in nucleoid morphology and origin localization upon inhibition or alteration of the actin homolog, MreB, of Vibrio cholerae.

artículo científico publicado en 2007

Co-expressed genes prepositioned in spatial neighborhoods stochastically associate with SC35 speckles and RNA polymerase II factories.

artículo científico publicado en 2013

Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter.

artículo científico publicado en 2008

Convergence of chromatin binding estimates in live cells.

artículo científico publicado en 2013

Crm1 is a mitotic effector of Ran-GTP in somatic cells

artículo científico publicado en 2005

Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates ⬇️

artículo científico publicado el 3 de noviembre de 2010

Design and In Vivo Immunogenicity of a Polyvalent Vaccine Based on SIVmac Regulatory Genes

article

Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching

artículo científico publicado en 2006

Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.

scientific article published on July 2009

Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis. ⬇️

artículo científico publicado en 2010

Dynamic behavior of transcription factors on a natural promoter in living cells

artículo científico publicado en 2002

Dynamic effect of bortezomib on nuclear factor-kappaB activity and gene expression in tumor cells.

artículo científico publicado en 2008

Dynamic interactions of a transcription factor with DNA are accelerated by a chromatin remodeller

artículo científico publicado en 2004

Dynamics of gene targeting and chromatin remodelling by nuclear receptors

artículo científico publicado en 2000

E-cadherin-mediated adhesion inhibits ligand-dependent activation of diverse receptor tyrosine kinases.

artículo científico publicado en 2004

Enhanced effector function of CD8(+) T cells from healthy controls and HIV-infected patients occurs through thrombin activation of protease-activated receptor 1.

artículo científico publicado en 2012

Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching.

artículo científico publicado en 2008

FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?

artículo científico publicado en 2010

Flow cytometric measurement of fluorescence (Förster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm.

artículo científico publicado en 2003

Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei

artículo científico publicado en 2004

Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser.

artículo científico publicado en 2003

Fractal geometry in the nucleus

artículo científico publicado en 2010

Generic features of tertiary chromatin structure as detected in natural chromosomes

artículo científico publicado en 2004

Genome-wide haploinsufficiency screen reveals a novel role for γ-TuSC in spindle organization and genome stability.

artículo científico publicado en 2013

Genome-wide protein-DNA binding dynamics suggest a molecular clutch for transcription factor function

artículo científico publicado en 2012

Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload

artículo científico publicado en 2011

Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation

artículo científico publicado en 2006

Human CD8+ T cells store RANTES in a unique secretory compartment and release it rapidly after TcR stimulation

artículo científico publicado en 2004

Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation.

artículo científico publicado en 2009

Intracellular delivery of acetyl-histone peptides inhibits native bromodomain-chromatin interactions and impairs mitotic progression.

artículo científico publicado en 2008

Jab1/CSN5, a component of the COP9 signalosome, regulates transforming growth factor beta signaling by binding to Smad7 and promoting its degradation

artículo científico publicado en 2004

Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter

artículo científico publicado en 2001

Measuring chromatin interaction dynamics on the second time scale at single-copy genes

artículo científico publicado en 2013

Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis

artículo científico publicado en 2012

Modeling a safer smallpox vaccination regimen, for human immunodeficiency virus type 1-infected patients, in immunocompromised macaques

artículo científico publicado en 2003

Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription

artículo científico publicado en 2004

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.

artículo científico publicado en 2012

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching.

artículo científico publicado en 2012

Monitoring dynamic binding of chromatin proteins in vivo by single-molecule tracking

artículo científico publicado en 2013

Novel functional dissection of the localization-specific roles of budding yeast polo kinase Cdc5p.

artículo científico publicado en 2004

Organization of chromatin and histone modifications at a transcription site.

artículo científico publicado en 2007

Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages.

artículo científico publicado en 2005

Photoswitching-free FRAP analysis with a genetically encoded fluorescent tag

artículo científico publicado en 2014

Potential Roles for Ubiquitin and the Proteasome during Ribosome Biogenesis

Potential roles for ubiquitin and the proteasome during ribosome biogenesis

artículo científico publicado en 2006

Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton.

artículo científico publicado en 2011

Quantifying transcription factor kinetics: at work or at play?

artículo científico

Quantitation of GFP-fusion proteins in single living cells

artículo científico publicado en 2002

Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes

artículo científico publicado en 2004

Regulation of RNA polymerase II activation by histone acetylation in single living cells.

artículo científico publicado en 2014

Regulation of TATA-binding protein dynamics in living yeast cells

artículo científico publicado en 2008

Requirement for the budding yeast polo kinase Cdc5 in proper microtubule growth and dynamics

artículo científico publicado en 2008

Role of H1 phosphorylation in rapid GR exchange and function at the MMTV promoter

artículo científico publicado en 2005

SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles

artículo científico publicado en 2002

Single molecule tracking of Ace1p in Saccharomyces cerevisiae defines a characteristic residence time for non-specific interactions of transcription factors with chromatin

artículo científico publicado en 2016

Single-Molecule Analysis Reveals Linked Cycles of RSC Chromatin Remodeling and Ace1p Transcription Factor Binding in Yeast

artículo científico publicado en 2018

Single-molecule analysis of transcription factor binding at transcription sites in live cells

artículo científico publicado en 2014

Smallpox vaccine safety is dependent on T cells and not B cells

artículo científico publicado en 2011

Stress-dependent proteolytic processing of the actin assembly protein Lsb1 modulates a yeast prion

artículo científico publicado en 2014

TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling

artículo científico publicado en 2003

TcR-induced regulated secretion leads to surface expression of CTLA-4 in CD4+CD25+ T cells

artículo científico publicado en 2008

The arylstibonic acid compound NSC13746 disrupts B-ZIP binding to DNA in living cells.

artículo científico publicado en 2010

The glucocorticoid receptor: rapid exchange with regulatory sites in living cells

artículo científico publicado en 2000

The interaction of NSBP1/HMGN5 with nucleosomes in euchromatin counteracts linker histone-mediated chromatin compaction and modulates transcription.

artículo científico publicado en 2009

Three-dimensional cellular ultrastructure resolved by X-ray microscopy.

artículo científico publicado en 2010

Three-dimensional imaging by deconvolution microscopy.

artículo científico publicado en 1999

Towards an atlas of mammalian cell ultrastructure by cryo soft X-ray tomography.

artículo científico publicado en 2011

Transcription dynamics

artículo científico publicado en 2009

Transcription factories

artículo científico publicado en 2012

iLAP: a workflow-driven software for experimental protocol development, data acquisition and analysis

artículo científico publicado en 2009

Lista de obras de

A benchmark for chromatin binding measurements in live cells.

A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET).

Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching.

Analysis of binding reactions by fluorescence recovery after photobleaching

Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy

Anti-angiogenic activity of human endostatin is HIF-1-independent in vitro and sensitive to timing of treatment in a human saphenous vein assay.

Assembly and function of the actin cytoskeleton of yeast: relationships between cables and patches.

Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription

CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites

Capping protein levels influence actin assembly and cell motility in dictyostelium

Cells Undergo Major Changes in the Quantity of Cytoplasmic Organelles after Uptake of Gold Nanoparticles with Biologically Relevant Surface Coatings

Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks

Changes in nucleoid morphology and origin localization upon inhibition or alteration of the actin homolog, MreB, of Vibrio cholerae.

Co-expressed genes prepositioned in spatial neighborhoods stochastically associate with SC35 speckles and RNA polymerase II factories.

Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter.

Convergence of chromatin binding estimates in live cells.

Crm1 is a mitotic effector of Ran-GTP in somatic cells

Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates ⬇️

Design and In Vivo Immunogenicity of a Polyvalent Vaccine Based on SIVmac Regulatory Genes

Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching

Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.

Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis. ⬇️

Dynamic behavior of transcription factors on a natural promoter in living cells

Dynamic effect of bortezomib on nuclear factor-kappaB activity and gene expression in tumor cells.

Dynamic interactions of a transcription factor with DNA are accelerated by a chromatin remodeller

Dynamics of gene targeting and chromatin remodelling by nuclear receptors

E-cadherin-mediated adhesion inhibits ligand-dependent activation of diverse receptor tyrosine kinases.

Enhanced effector function of CD8(+) T cells from healthy controls and HIV-infected patients occurs through thrombin activation of protease-activated receptor 1.

Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching.

FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?

Flow cytometric measurement of fluorescence (Förster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm.

Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei

Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser.

Fractal geometry in the nucleus

Generic features of tertiary chromatin structure as detected in natural chromosomes

Genome-wide haploinsufficiency screen reveals a novel role for γ-TuSC in spindle organization and genome stability.

Genome-wide protein-DNA binding dynamics suggest a molecular clutch for transcription factor function

Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload

Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation

Human CD8+ T cells store RANTES in a unique secretory compartment and release it rapidly after TcR stimulation

Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation.

Intracellular delivery of acetyl-histone peptides inhibits native bromodomain-chromatin interactions and impairs mitotic progression.

Jab1/CSN5, a component of the COP9 signalosome, regulates transforming growth factor beta signaling by binding to Smad7 and promoting its degradation

Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter

Measuring chromatin interaction dynamics on the second time scale at single-copy genes

Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis

Modeling a safer smallpox vaccination regimen, for human immunodeficiency virus type 1-infected patients, in immunocompromised macaques

Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching.

Monitoring dynamic binding of chromatin proteins in vivo by single-molecule tracking

Novel functional dissection of the localization-specific roles of budding yeast polo kinase Cdc5p.

Organization of chromatin and histone modifications at a transcription site.

Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages.

Photoswitching-free FRAP analysis with a genetically encoded fluorescent tag

Potential Roles for Ubiquitin and the Proteasome during Ribosome Biogenesis

Potential roles for ubiquitin and the proteasome during ribosome biogenesis

Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton.

Quantifying transcription factor kinetics: at work or at play?

Quantitation of GFP-fusion proteins in single living cells

Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes

Regulation of RNA polymerase II activation by histone acetylation in single living cells.

Regulation of TATA-binding protein dynamics in living yeast cells

Requirement for the budding yeast polo kinase Cdc5 in proper microtubule growth and dynamics

Role of H1 phosphorylation in rapid GR exchange and function at the MMTV promoter

SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles

Single molecule tracking of Ace1p in Saccharomyces cerevisiae defines a characteristic residence time for non-specific interactions of transcription factors with chromatin

Single-Molecule Analysis Reveals Linked Cycles of RSC Chromatin Remodeling and Ace1p Transcription Factor Binding in Yeast

Single-molecule analysis of transcription factor binding at transcription sites in live cells

Smallpox vaccine safety is dependent on T cells and not B cells

Stress-dependent proteolytic processing of the actin assembly protein Lsb1 modulates a yeast prion

TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling

TcR-induced regulated secretion leads to surface expression of CTLA-4 in CD4+CD25+ T cells

The arylstibonic acid compound NSC13746 disrupts B-ZIP binding to DNA in living cells.

The glucocorticoid receptor: rapid exchange with regulatory sites in living cells

The interaction of NSBP1/HMGN5 with nucleosomes in euchromatin counteracts linker histone-mediated chromatin compaction and modulates transcription.

Three-dimensional cellular ultrastructure resolved by X-ray microscopy.

Three-dimensional imaging by deconvolution microscopy.

Towards an atlas of mammalian cell ultrastructure by cryo soft X-ray tomography.