Lista de obras - Radka Chaloupkova - Dominio Público Uruguay

Ancestral Haloalkane Dehalogenases Show Robustness and Unique Substrate Specificity.

artículo científico publicado en 2017

Are time-dependent fluorescence shifts at the tunnel mouth of haloalkane dehalogenase enzymes dependent on the choice of the chromophore?

artículo científico publicado en 2013

Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium ⬇️

artículo científico publicado el 11 de mayo de 2012

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

artículo científico publicado en 2010

Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

artículo científico publicado en 2009

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling

artículo científico publicado en 2017

Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

artículo científico publicado en 2003

Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases

artículo científico publicado en 2005

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2.

artículo científico publicado en 2017

Construction of cryptogein mutants, a proteinaceous elicitor from Phytophthora, with altered abilities to induce a defense reaction in tobacco cells.

artículo científico publicado en 2005

Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5

scientific article published on 24 April 2019

Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands.

artículo científico publicado en 2011

Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31

artículo científico publicado en 2014

Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.

artículo científico publicado en 2013

Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17

scientific article published on 28 October 2019

Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites.

artículo científico publicado en 2017

DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin ⬇️

artículo científico publicado el 11 de agosto de 2013

Elicitin–membrane interaction is driven by a positive charge on the protein surface: Role of Lys13 residue in lipids loading and resistance induction

artículo científico publicado en 2011

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

artículo científico publicado en 2013

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

artículo científico publicado en 2015

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents

artículo científico publicado en 2013

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

artículo científico publicado en 2015

Functional analysis of the aglycone-binding site of the maize beta-glucosidase Zm-p60.1.

artículo científico publicado en 2008

Gram-scale production of recombinant microbial enzymes in shake flasks.

artículo científico publicado en 2017

Haloalkane dehalogenases: biotechnological applications.

artículo científico

Impact of the access tunnel engineering on catalysis is strictly ligand-specific

artículo científico publicado en 2018

Instability restricts signaling of multiple fibroblast growth factors.

artículo científico publicado en 2015

Kinetics of binding of fluorescent ligands to enzymes with engineered access tunnels.

artículo científico publicado en 2016

Lincomycin biosynthesis involves a tyrosine hydroxylating heme protein of an unusual enzyme family

artículo científico publicado en 2013

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

artículo científico publicado en 2003

NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus

artículo científico publicado en 2009

Organic co-solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases

artículo científico publicado en 2013

Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.

artículo científico publicado en 2009

Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance.

artículo científico publicado en 2012

Rad52 SUMOylation affects the efficiency of the DNA repair

artículo científico publicado en 2010

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

artículo científico publicado en 2009

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

artículo científico publicado en 2013

Site-Specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence

artículo científico publicado en 2015

Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature ⬇️

artículo científico publicado el 27 de junio de 2011

Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites

artículo científico publicado en 2014

Study of enzymatic reaction by electrophoretically mediated microanalysis in a partially filled capillary with indirect or direct detection.

artículo científico publicado en 2004

Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary

scientific article published on 29 September 2006

Suppression of protein inactivation during freezing by minimizing pH changes using ionic cryoprotectants

artículo científico publicado en 2016

The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice

artículo científico publicado en 2016

The effect of a unique halide-stabilizing residue on the catalytic properties of haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58.

artículo científico publicado en 2013

Lista de obras de Radka Chaloupkova

Ancestral Haloalkane Dehalogenases Show Robustness and Unique Substrate Specificity.

Are time-dependent fluorescence shifts at the tunnel mouth of haloalkane dehalogenase enzymes dependent on the choice of the chromophore?

Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium ⬇️

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling

Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2.

Construction of cryptogein mutants, a proteinaceous elicitor from Phytophthora, with altered abilities to induce a defense reaction in tobacco cells.

Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5

Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands.

Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31

Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.

Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17

Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites.

DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin ⬇️

Elicitin–membrane interaction is driven by a positive charge on the protein surface: Role of Lys13 residue in lipids loading and resistance induction

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

Functional analysis of the aglycone-binding site of the maize beta-glucosidase Zm-p60.1.

Gram-scale production of recombinant microbial enzymes in shake flasks.

Haloalkane dehalogenases: biotechnological applications.

Impact of the access tunnel engineering on catalysis is strictly ligand-specific

Instability restricts signaling of multiple fibroblast growth factors.

Kinetics of binding of fluorescent ligands to enzymes with engineered access tunnels.

Lincomycin biosynthesis involves a tyrosine hydroxylating heme protein of an unusual enzyme family

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus

Organic co-solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases

Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.

Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance.

Rad52 SUMOylation affects the efficiency of the DNA repair

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

Site-Specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence

Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature ⬇️

Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites

Study of enzymatic reaction by electrophoretically mediated microanalysis in a partially filled capillary with indirect or direct detection.

Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary

Suppression of protein inactivation during freezing by minimizing pH changes using ionic cryoprotectants

The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice

The effect of a unique halide-stabilizing residue on the catalytic properties of haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58.