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Lista de obras de Michael W Pfaffl

A method to assess 59Fe in residual tissue blood content in mice and its use to correct 59Fe-distribution kinetics accordingly

artículo científico publicado en 2007

A new mathematical model for relative quantification in real-time RT-PCR

artículo científico publicado en 2001

Analysis of key molecules of the innate immune system in mammary epithelial cells isolated from marker-assisted and conventionally selected cattle.

artículo científico publicado en 2009

Angiogenesis in The Ovary - The Most Important Regulatory Event for Follicle and Corpus Luteum Development and Function in Cow - An Overview.

artículo científico publicado en 2015

Blastocysts exhibit sex-specific signalling of IFNT transcription, translation and activity

scientific article published on 01 March 2019

Can milk cell or skim milk miRNAs be used as biomarkers for early pregnancy detection in cattle?

artículo científico publicado en 2017

Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis.

artículo científico publicado en 2017

Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy

artículo científico publicado en 2018

Changes in the miRNA profile under the influence of anabolic steroids in bovine liver.

artículo científico publicado en 2011

Changes in the microRNA expression profile during blood storage

scientific article published on 05 July 2018

Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds.

artículo científico publicado en 2003

Class I odorant receptors, TAS1R and TAS2R taste receptors, are markers for subpopulations of circulating leukocytes

artículo científico publicado en 2015

Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research.

artículo científico publicado en 2019

Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

artículo científico publicado en 2006

Comparison of reverse transcriptases in gene expression analysis.

artículo científico publicado en 2004

Comparison of the immune competence of Turopolje, German Landrace × Turopolje, and German Landrace × Pietrain pigs after PRRSV vaccination

artículo científico publicado en 2016

Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing.

artículo científico publicado en 2015

Detection and quantification of mRNA expression of alpha- and beta-adrenergic receptor subtypes in the mammary gland of dairy cows

artículo científico publicado en 2003

Detection of Atherosclerosis by Small RNA-Sequencing Analysis of Extracellular Vesicle Enriched Serum Samples

artículo científico publicado en 2021

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

artículo científico publicado en 2004

Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 2: certification.

artículo científico publicado en 2001

Differential expression of molecular motors in the motor cortex of sporadic ALS

artículo científico publicado en 2007

Distribution-insensitive cluster analysis in SAS on real-time PCR gene expression data of steadily expressed genes.

artículo científico publicado en 2006

EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research.

artículo científico publicado en 2017

Editorial

artículo científico publicado en 2019

Effect of Perilla frutescens Extracts on Porcine Jejunal Epithelial Cells.

artículo científico publicado en 2016

Effect of insoluble fibre on intestinal morphology and mRNA expression pattern of inflammatory, cell cycle and growth marker genes in a piglet model.

artículo científico publicado en 2008

Effect of lactoferrin on selected immune system parameters and the gastrointestinal morphology in growing calves

artículo científico publicado en 2007

Effect of lactulose on growth performance and intestinal morphology of pre-ruminant calves using a milk replacer containing Enterococcus faecium

artículo científico publicado en 2007

Effect of non-starch-polysaccharide-degrading enzymes as feed additive on the rumen bacterial population in non-lactating cows quantified by real-time PCR.

artículo científico publicado en 2012

Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells.

artículo científico publicado en 2016

Effect of trenbolone acetate plus estradiol on transcriptional regulation of metabolism pathways in bovine liver

artículo científico publicado en 2010

Effects of inulin and lactulose on the intestinal morphology of calves

artículo científico publicado en 2010

Effects of lactoferrin feeding on growth, feed intake and health of calves

artículo científico publicado en 2007

Effects of muscle type, castration, age, and compensatory growth rate on androgen receptor mRNA expression in bovine skeletal muscle.

artículo científico publicado en 2000

Effects of mycophenolic acid on inosine monophosphate dehydrogenase I and II mRNA expression in white blood cells and various tissues in sheep

artículo científico publicado en 2006

Effects of synthetic progestagens on the mRNA expression of androgen receptor, progesterone receptor, oestrogen receptor alpha and beta, insulin-like growth factor-1 (IGF-1) and IGF-1 receptor in heifer tissues.

artículo científico publicado en 2002

Effects of the prebiotics inulin and lactulose on intestinal immunology and hematology of preruminant calves.

artículo científico publicado en 2011

Electric cell-substrate impedance sensing (ECIS) based real-time measurement of titer dependent cytotoxicity induced by adenoviral vectors in an IPI-2I cell culture model ⬇️

artículo científico publicado el 9 de septiembre de 2010

Elevated caspase-3 and Fas mRNA expression in jejunum of adult rats during subclinical zinc deficiency

artículo científico publicado en 2004

Erratum: Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by Next-Generation Sequencing

artículo científico publicado en 2019

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk.

artículo científico publicado en 2017

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications

artículo científico publicado en 2013

Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing.

artículo científico publicado en 2018

Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution ⬇️

artículo científico publicado el 1 de mayo de 2003

Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.

artículo científico publicado en 2016

Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy.

artículo científico publicado en 2002

Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary.

artículo científico publicado en 2016

Gene expression profiling in pbMEC - in search of molecular biomarkers to predict immunoglobulin production in bovine milk

artículo científico publicado en 2017

Glucocorticoid receptor overexpression slightly shifts microRNA expression patterns in triple-negative breast cancer.

artículo científico publicado en 2018

Grass pollen production and group V allergen content of agriculturally relevant species and cultivars

artículo científico publicado en 2018

Growth Hormone Secretion Patterns in German Landrace (DL) Fetuses and Piglets Compared to DL Piglets with Inherited 1,25-Dihydroxyvitamin D3 Deficiency.

artículo científico publicado en 2018

Guest editor's introduction for BDQ special issue: 'Advanced Molecular Diagnostics for Biomarker Discovery'.

artículo científico publicado en 2015

Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication.

artículo científico publicado en 2017

Highlights of the mini-symposium on extracellular vesicles in inter-organismal communication, held in Munich, Germany, August 2018

article

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

artículo científico publicado en 2015

Human airway epithelial extracellular vesicle miRNA signature is altered upon asthma development

artículo científico publicado en 2019

Identification of a piscine reovirus-related pathogen in proliferative darkening syndrome (PDS) infected brown trout (Salmo trutta fario) using a next-generation technology detection pipeline

scientific article published in PLoS ONE

Identification of a potential gene expression biomarker signature in bovine liver to detect the abuse of growth promoters.

artículo científico publicado en 2014

Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells.

artículo científico publicado en 2009

Impact of elevated air temperature and drought on pollen characteristics of major agricultural grass species

artículo científico publicado en 2021

Impact of preimplantational oral low-dose estradiol-17β exposure on the endometrium: the role of miRNA.

artículo científico publicado en 2018

Influence of anabolic combinations of an androgen plus an estrogen on biochemical pathways in bovine uterine endometrium and ovary.

artículo científico publicado en 2011

Influence of oral application of mycophenolic acid on the clinical health status of sheep

artículo científico publicado en 2007

Influence of testosterone and a novel SARM on gene expression in whole blood of Macaca fascicularis.

artículo científico publicado en 2009

Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats.

artículo científico publicado en 2003

Inhibition of fat cell differentiation in 3T3-L1 pre-adipocytes by all-trans retinoic acid: Integrative analysis of transcriptomic and phenotypic data

artículo científico publicado en 2016

Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants.

artículo científico publicado en 2004

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

artículo científico publicado en 2003

Integrative Analysis of MicroRNA and mRNA Data Reveals an Orchestrated Function of MicroRNAs in Skeletal Myocyte Differentiation in Response to TNF-α or IGF1.

artículo científico publicado en 2015

Inulin and probiotics in newly weaned piglets: effects on intestinal morphology, mRNA expression levels of inflammatory marker genes and haematology

artículo científico publicado en 2010

Involvement of Pro-Inflammatory Cytokines, Mediators of Inflammation, and Basic Fibroblast Growth Factor in Prostaglandin F2α-Induced Luteolysis in Bovine Corpus Luteum1 ⬇️

artículo científico publicado el 15 de octubre de 2003

Letter to The Editor: MicroRNA Profile and Adaptive Response to Exercise Training: A Review

scientific article published on 06 September 2019

Long-term effects of mycophenolic acid on the immunoglobulin and inflammatory marker-gene expression in sheep white blood cells

artículo científico publicado en 2010

MicroRNA of whole milk samples are not suitable for pregnancy detection in cattle

scientific article published on 11 January 2019

Milking characteristics and their relation to adrenergic receptor mRNA expression and ligand binding in the mammary gland of dairy cows.

artículo científico publicado en 2003

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

scientific article published on 23 November 2018

Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers

scientific article published on 07 November 2006

Monitoring gene expression in muscle tissue of macaca fascicularis under the influence of testosterone and SARM.

artículo científico publicado en 2010

New surveillance concepts in food safety in meat producing animals: the advantage of high throughput 'omics' technologies - A review

artículo científico publicado en 2018

Nicotinic acetylcholine receptor subunits α4 and α5 associated with smoking behaviour and lung cancer are regulated by upstream open reading frames.

artículo científico publicado en 2013

Non-invasive screening for treatment of heifers with the anabolic steroid melengestrol acetate (MGA) by feces analysis.

artículo científico publicado en 2003

Normalization strategies for microRNA profiling experiments: a 'normal' way to a hidden layer of complexity?

artículo científico publicado en 2010

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper

artículo científico publicado en 2017

Optimization of extraction of circulating RNAs from plasma--enabling small RNA sequencing

artículo científico publicado en 2014

Pleading for adherence to the MIQE-Guidelines when reporting quantitative PCR data in forensic genetic research

scientific article published on 26 June 2019

Postprandial transfer of colostral extracellular vesicles and their protein and miRNA cargo in neonatal calves

scientific article published on 28 February 2020

Posttranscriptional regulatory networks: from expression profiling to integrative analysis of mRNA and microRNA data.

artículo científico publicado en 2014

Primer sequence disclosure: a clarification of the MIQE guidelines.

artículo científico publicado en 2011

Production of CTC-containing porcine reference materials

artículo científico publicado en 1998

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study

artículo científico publicado en 2012

Progranulin signaling in sepsis, community-acquired bacterial pneumonia and COVID-19: a comparative, observational study

artículo científico publicado en 2021

Prostaglandins in Superovulation Induced Bovine Follicles During the Preovulatory Period and Early Corpus Luteum

scientific article published on 10 July 2019

Quality control for quantitative PCR based on amplification compatibility test.

artículo científico publicado en 2010

Quantification noise in single cell experiments.

artículo científico publicado en 2011

Quantification of androgen receptor mRNA in tissues by competitive co-amplification of a template in reverse transcription-polymerase chain reaction

artículo científico publicado en 1996

Quantification of insulin-like growth factor-1 (IGF-1) mRNA: development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction

artículo científico publicado en 1998

Quantification of insulin-like growth factor-1 (IGF-1) mRNA: modulation of growth intensity by feeding results in inter- and intra-tissue-specific differences of IGF-1 mRNA expression in steers.

artículo científico publicado en 1998

Quantitative mRNA analysis of eight bovine 5-HT receptor subtypes in brain, abomasum, and intestine by real-time RT-PCR.

artículo científico publicado en 2003

Quantitative real-time RT-PCR--a perspective.

artículo científico publicado en 2005

RDML-Ninja and RDMLdb for standardized exchange of qPCR data

artículo científico publicado en 2015

RNA integrity and the effect on the real-time qRT-PCR performance

artículo científico publicado en 2006

RNA-Sequencing as Useful Screening Tool in the Combat against the Misuse of Anabolic Agents

artículo científico publicado en 2012

Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species

artículo científico publicado en 2002

RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

artículo científico publicado en 2011

Reference histology of veal calf genital and endocrine tissues - an update for screening on hormonal growth promoters.

artículo científico publicado en 2007

Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

artículo científico publicado en 2002

Reply

artículo científico publicado en 2016

Shisha microbiota: the good, the bad and the not so ugly.

artículo científico publicado en 2018

Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis.

artículo científico publicado en 2004

Short-term effects on pro-inflammatory cytokine, lactoferrin and CD14 mRNA expression levels in bovine immunoseparated milk and blood cells treated by LPS.

artículo científico publicado en 2005

Standardized determination of real-time PCR efficiency from a single reaction set-up

artículo científico publicado en 2003

Stimulated enrichment of Clostridium difficile specific IgA in mature cow's milk.

artículo científico publicado en 2018

Synergetic downregulation of 67 kDa laminin receptor by the green tea (Camellia sinensis) secondary plant compound epigallocatechin gallate: a new gateway in metastasis prevention?

artículo científico publicado en 2012

TNF-α and IGF1 modify the microRNA signature in skeletal muscle cell differentiation

artículo científico publicado en 2015

Tailoring the resolution of single-cell RNA sequencing for primary cytotoxic T cells

artículo científico publicado en 2021

The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression

artículo científico publicado en 2017

The Emerging Role of miRNAs for the Radiation Treatment of Pancreatic Cancer

artículo científico publicado en 2020

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

artículo científico publicado en 2009

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

artículo científico publicado en 2013

The effects of branched-chain amino acid interactions on growth performance, blood metabolites, enzyme kinetics and transcriptomics in weaned pigs.

artículo científico publicado en 2010

The gastrointestinal tract as target of steroid hormone action: quantification of steroid receptor mRNA expression (AR, ERalpha, ERbeta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR.

artículo científico publicado en 2003

The immunomodulatory effect of lactulose on Enterococcus faecium fed preruminant calves.

artículo científico publicado en 2008

The influence of polyphenol rich apple pomace or red-wine pomace diet on the gut morphology in weaning piglets.

artículo científico publicado en 2007

The mRNA expression of insulin receptor isoforms (IR-A and IR-B) and IGFR-2 in the bovine corpus luteum during the estrous cycle, pregnancy, and induced luteolysis

artículo científico publicado en 2003

The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis.

artículo científico publicado en 2003

The need for transparency and good practices in the qPCR literature.

artículo científico publicado en 2013

The ongoing evolution of qPCR.

artículo científico publicado en 2010

The physiological way: monitoring RNA expression changes as new approach to combat illegal growth promoter application

artículo científico publicado en 2012

The potential of bovine vaginal smear for biomarker development to trace the misuse of anabolic agents.

artículo científico publicado en 2010

The potential of circulating extracellular small RNAs (smexRNA) in veterinary diagnostics-Identifying biomarker signatures by multivariate data analysis

artículo científico publicado en 2015

The use of omic technologies for biomarker development to trace functions of anabolic agents

artículo científico publicado en 2009

Tissue-specific expression pattern of bovine prion gene: quantification using real-time RT-PCR.

artículo científico publicado en 2003

Tissue-specific expression pattern of estrogen receptors (ER): quantification of ER alpha and ER beta mRNA with real-time RT-PCR.

artículo científico publicado en 2001

Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow

artículo científico publicado en 2016

Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods

scientific article published on 04 September 2012

Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera

scientific article published on 27 September 2019

Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model

artículo científico publicado en 2019

Tumor Necrosis Factor Alpha and Insulin-Like Growth Factor 1 Induced Modifications of the Gene Expression Kinetics of Differentiating Skeletal Muscle Cells

artículo científico publicado en 2015

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

artículo científico publicado en 2009

Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses

artículo científico publicado en 2009

[Insulin-like growth factor 1 (IGF-1) mRNA and IGF-1 protein. Expression in cells of the trabecular meshwork of the bovine eye]

artículo científico publicado en 2002

mRNA and microRNA purity and integrity: the key to success in expression profiling.

artículo científico publicado en 2014

miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity

artículo científico publicado en 2016

microRNA in native and processed cow's milk and its implication for the farm milk effect on asthma

artículo científico publicado en 2015

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