Filtros de búsqueda

Lista de obras de Paul A Dalby

A Multidisciplinary Approach Toward the Rapid and Preparative-Scale Biocatalytic Synthesis of Chiral Amino Alcohols: A Concise Transketolase-/ω-Transaminase-Mediated Synthesis of (2S,3S)-2-Aminopentane-1,3-diol

A critical assessment of the impact of mixing on dilution refolding.

artículo científico publicado en 2006

A generic hierarchical screening method for the analysis of microscale refolds using an automated robotic platform

artículo científico publicado el 13 de enero de 2012

A high-throughput fluorescence chemical denaturation assay as a general screen for protein-ligand binding

scientific article published on 05 December 2010

A microplate-based evaluation of complex denaturation pathways: structural stability of Escherichia coli transketolase.

artículo científico publicado en 2008

Accelerated design of bioconversion processes using automated microscale processing techniques

artículo científico publicado en 2003

An Evaluation of the Potential of NMR Spectroscopy and Computational Modelling Methods to Inform Biopharmaceutical Formulations

scientific article published on 21 September 2018

An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates

artículo científico publicado en 2017

Better Biocatalytic Processes Faster: New Tools for the Implementation of Biocatalysis in Organic Synthesis

Computational Design To Reduce Conformational Flexibility and Aggregation Rates of an Antibody Fab Fragment

artículo científico publicado en 2018

Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

article published in the Proceedings of the National Academy of Sciences of the United States of America

Directed evolution of a thermostable l-aminoacylase biocatalyst.

artículo científico publicado en 2011

Directed evolution of biocatalytic processes

artículo científico publicado en 2005

Directed evolution of transketolase activity on non-phosphorylated substrates.

artículo científico publicado en 2007

Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde

artículo científico publicado en 2008

Directed evolution strategies for improved enzymatic performance

artículo científico publicado en 2005

Directed evolution to re-adapt a co-evolved network within an enzyme.

artículo científico publicado en 2011

Distributions of enzyme residues yielding mutants with improved substrate specificities from two different directed evolution strategies

artículo científico publicado en 2009

Engineering and design

artículo científico publicado en 2011

Evolutionary analysis of the TPP-dependent enzyme family.

artículo científico publicado en 2007

Exploiting correlated molecular-dynamics networks to counteract enzyme activity-stability trade-off

article published in the Proceedings of the National Academy of Sciences of the United States of America

Exposing relationships using directed evolution.

artículo científico publicado en 2004

Factors affecting protein refolding yields in a fed-batch and batch-refolding system.

artículo científico publicado en 2007

Fine-tuning the activity and stability of an evolved enzyme active-site through non-canonical amino-acids

artículo científico publicado en 2020

Folding intermediates of wild-type and mutants of barnase. II. correlation of changes in equilibrium amide exchange kinetics with the population of the folding intermediate

artículo científico publicado el 27 de febrero de 1998

HDX and In Silico Docking Reveal that Excipients Stabilize G-CSF via a Combination of Preferential Exclusion and Specific Hotspot Interactions

artículo científico publicado en 2020

Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes

artículo científico publicado en 2014

Identification of Protein-Excipient Interaction Hotspots Using Computational Approaches

artículo científico publicado en 2016

Impact of cofactor-binding loop mutations on thermotolerance and activity of E. coli transketolase.

artículo científico publicado en 2016

Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment.

artículo científico publicado en 2015

Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy

article

Movement of the Intermediate and Rate Determining Transition State of Barnase on the Energy Landscape with Changing Temperature

artículo científico publicado el 31 de marzo de 1998

NMR Reveals Functionally Relevant Thermally Induced Structural Changes within the Native Ensemble of G-CSF

artículo científico publicado en 2022

Non-α-hydroxylated aldehydes with evolved transketolase enzymes

artículo científico publicado en 2010

Novel insights into transketolase activation by cofactor binding identifies two native species subpopulations

scientific article published on 06 November 2019

One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine: pyruvate transaminase pathway in Escherichia coli.

artículo científico publicado en 2007

Optically induced thermal gradients for protein characterization in nanolitre-scale samples in microfluidic devices

artículo científico publicado en 2013

Optimal synthesis of chromatographic trains for downstream protein processing.

artículo científico publicado en 2011

Optimisation and evaluation of a generic microplate-based HPLC screen for transketolase activity

artículo científico publicado en 2007

Optimising enzyme function by directed evolution.

artículo científico publicado en 2003

Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale

artículo científico publicado en 2010

Rapid optimization of protein freeze-drying formulations using ultra scale-down and factorial design of experiment in microplates.

artículo científico publicado en 2009

Rational substrate and enzyme engineering of transketolase for aromatics.

artículo científico publicado en 2012

Second generation engineering of transketolase for polar aromatic aldehyde substrates.

artículo científico publicado en 2015

Selective Stabilization and Destabilization of Protein Domains in Tissue-Type Plasminogen Activator Using Formulation Excipients

artículo científico publicado en 2019

Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase

artículo científico publicado en 2015

Strategy and success for the directed evolution of enzymes

artículo científico publicado el 16 de junio de 2011

Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes.

artículo científico publicado en 2016

Structural stability of E. coli transketolase to temperature and pH denaturation.

artículo científico publicado en 2011

Structural stability of an enzyme biocatalyst.

artículo científico publicado en 2007

The Fab conformations in the solution structure of human immunoglobulin G4 (IgG4) restrict access to its Fc region: implications for functional activity

artículo científico publicado en 2014

The Two-Species Model of transketolase explains donor substrate-binding, inhibition and heat-activation

artículo científico publicado en 2020

The solution structure of rabbit IgG accounts for its interactions with the Fc receptor and complement C1q and its conformational stability.

artículo científico publicado en 2012

The solution structures of two human IgG1 antibodies show conformational stability and accommodate their C1q and FcγR ligands.

artículo científico publicado en 2015

Thermodynamic parameters for salt‐induced reversible protein precipitation from automated microscale experiments

artículo científico publicado el 1 de febrero de 2011

Tm-Values and Unfolded Fraction Can Predict Aggregation Rates for Granulocyte Colony Stimulating Factor Variant Formulations but Not under Predominantly Native Conditions.

artículo científico publicado en 2017

Two strategies to engineer flexible loops for improved enzyme thermostability.

artículo científico publicado en 2017

Ultra scale-down of protein refold screening in microwells: challenges, solutions and application

artículo científico publicado en 2009

Virus lasers for biological detection

scientific article published on 09 August 2019

Yeast-surface expressed BVDV E2 protein induces a Th1/Th2 response in naïve T cells.

artículo científico publicado en 2011

α,α′-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes

artículo científico publicado el 10 de septiembre de 2010