Lista de obras - Renate Kunert - Dominio Público Uruguay

A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples

artículo científico publicado en 2017

A novel strategy for quantitative isoform detection directly performed from culture supernatant.

artículo científico publicado en 2006

A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits

artículo científico publicado en 2008

A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1.

artículo científico publicado en 2001

A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells

artículo científico publicado en 2012

A study on the temperature dependency and time course of the cold capture antibody secretion assay

artículo científico publicado en 2009

Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

artículo científico publicado en 2014

Advances in recombinant antibody manufacturing

artículo científico publicado en 2016

Analysis of changes during subclone development and ageing of human antibody-producing heterohybridoma cells by northern blot and flow cytometry.

artículo científico publicado en 1999

Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides

artículo científico publicado en 2008

Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1.

artículo científico publicado en 2005

Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5

article

Anti-neuroblastoma effect of ch14.18 antibody produced in CHO cells is mediated by NK-cells in mice.

artículo científico publicado en 2005

Antibody domain exchange is an immunological solution to carbohydrate cluster recognition

artículo científico publicado en 2003

Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations

artículo científico publicado en 2016

Applicability of different fluorescent dyes for isoform quantification on linear IPG gels

artículo científico publicado en 2007

Benchmarking of commercially available CHO cell culture media for antibody production.

artículo científico publicado en 2015

Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changes.

artículo científico publicado en 2004

Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells.

artículo científico publicado en 2006

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

artículo científico publicado en 2018

Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression.

artículo científico publicado en 2016

CHO-recombinant human growth hormone as a protease sensitive reporter protein.

artículo científico publicado en 2009

Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies.

artículo científico publicado en 2005

Ch14.18 antibody produced in CHO cells in relapsed or refractory Stage 4 neuroblastoma patients: a SIOPEN Phase 1 study.

artículo científico publicado en 2013

Changes during subclone development and ageing of human antibody-producing recombinant CHO cells

artículo científico publicado en 1999

Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters.

artículo científico publicado en 2006

Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12.

artículo científico publicado en 2003

Characterization of molecular features, antigen-binding, and in vitro properties of IgG and IgM variants of 4E10, an anti-HIV type 1 neutralizing monoclonal antibody.

artículo científico publicado en 2004

Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression

artículo científico publicado en 2017

Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies

artículo científico publicado en 2004

Cross-Neutralizing Human Monoclonal Anti-HIV-1 Antibody 2F5: Preparation and Crystallographic Analysis of the Free and Epitope-Complexed Forms of its F ab Fragment

article

Crystal structure of the complex between the F(ab)' fragment of the cross-neutralizing anti-HIV-1 antibody 2F5 and the F(ab) fragment of its anti-idiotypic antibody 3H6

artículo científico publicado en 2008

Differential gene expression of a feed-spiked super-producing CHO cell line

article

Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line

artículo científico publicado en 2005

Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12.

artículo científico publicado en 2004

Engineering of Pichia pastoris for improved production of antibody fragments.

artículo científico publicado en 2006

Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting

artículo científico publicado en 2005

Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

artículo científico publicado en 2014

Evaluation of the potency of the anti-idiotypic antibody Ab2/3H6 mimicking gp41 as an HIV-1 vaccine in a rabbit prime/boost study

artículo científico publicado en 2012

Exploration of BAC versus plasmid expression vectors in recombinant CHO cells.

artículo científico publicado en 2012

Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis

article by Andreas Loos et al published April 2011 in Plant Physiology

Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12.

artículo científico publicado en 2010

Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures.

artículo científico publicado en 2007

Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients.

artículo científico publicado en 2014

Glycan profile of CHO derived IgM purified by highly efficient single step affinity chromatography.

artículo científico publicado en 2017

Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth

artículo científico publicado en 2011

Heterologous protein production using euchromatin-containing expression vectors in mammalian cells

artículo científico publicado en 2015

High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris

article

Humanization strategies for an anti-idiotypic antibody mimicking HIV-1 gp41 ⬇️

artículo científico publicado el 30 de octubre de 2010

Identification of bottlenecks in antibody expression using targeted gene integration.

artículo científico publicado en 2015

Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation

artículo científico publicado en 2014

Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH.

artículo científico publicado en 2006

IgM characterization directly performed in crude culture supernatants by a new simple electrophoretic method.

artículo científico publicado en 2010

Impact of temperature and pH on recombinant human IgM quality attributes and productivity

scientific article published on 07 January 2019

Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

artículo científico publicado en 2015

Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile

scientific article published on 28 May 2009

Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

artículo científico publicado en 2007

In planta protein sialylation through overexpression of the respective mammalian pathway

artículo científico publicado en 2010

In search of expression bottlenecks in recombinant CHO cell lines--a case study.

artículo científico publicado en 2014

Inefficient CAR-proximal signaling blunts antigen sensitivity

artículo científico publicado en 2020

Influence of cell culture media and feed supplements on cell metabolism and quality of IgG produced in CHO-K1, CHO-S, and CHO-DG44.

artículo científico publicado en 2015

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

artículo científico publicado en 2010

Inhibition of human immunodeficiency virus type 1 entry in cells expressing gp41-derived peptides

artículo científico publicado en 2004

Inhibition of in vivo HIV infection in humanized mice by gene therapy of human hematopoietic stem cells with a lentiviral vector encoding a broadly neutralizing anti-HIV antibody

artículo científico publicado en 2010

Interaction of anti-HIV type 1 antibody 2F5 with phospholipid bilayers and its relevance for the mechanism of virus neutralization

artículo científico publicado en 2011

Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM

artículo científico publicado en 2015

Lessons learned from merging wet lab experiments with molecular simulation to improve mAb humanization

artículo científico publicado en 2018

Membrane association and epitope recognition by HIV-1 neutralizing anti-gp41 2F5 and 4E10 antibodies.

artículo científico publicado en 2006

Molecular Characterization of Five Neutralizing Anti-HIV Type 1 Antibodies: Identification of Nonconventional D Segments in the Human Monoclonal Antibodies 2G12 and 2F5

article

Molecular Simulations to Rationalize Humanized Ab2/3H6 Activity

Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control

scientific article published on 02 April 2018

Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering

artículo científico publicado en 2011

Nomenclature of humanized mAbs: Early concepts, current challenges and future perspectives

scholarly article by Patrick Mayrhofer & Renate Kunert published 23 July 2018 in Human Antibodies

Novel bifunctional single-chain variable antibody fragments to enhance virolysis by complement: generation and proof-of-concept

artículo científico publicado en 2014

Novel human renal proximal tubular cell line for the production of complex proteins.

artículo científico publicado en 2014

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

artículo científico publicado en 2014

Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors

artículo científico publicado en 2006

Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells

artículo científico publicado en 2006

Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern

artículo científico publicado en 2007

Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana

article

Proteomic differences in recombinant CHO cells producing two similar antibody fragments.

artículo científico publicado en 2016

Rapid development of clone-specific, high-performing perfusion media from established feed supplements

scientific article published on 11 November 2019

Recent advances in recombinant protein production: BAC-based expression vectors, the bigger the better.

artículo científico publicado en 2013

Recognition and blocking of HIV-1 gp41 pre-transmembrane sequence by monoclonal 4E10 antibody in a Raft-like membrane environment

artículo científico publicado en 2006

Recognition of membrane-bound fusion-peptide/MPER complexes by the HIV-1 neutralizing 2F5 antibody: implications for anti-2F5 immunogenicity

artículo científico publicado en 2012

Recombinant IgA production: single step affinity purification using camelid ligands and product characterization.

artículo científico publicado en 2012

Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans.

artículo científico publicado en 2007

Screening for improved cell performance: selection of subclones with altered production kinetics or improved stability by cell sorting

artículo científico publicado en 2004

Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection.

artículo científico publicado en 2009

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

artículo científico publicado en 2007

Stable overexpression of miR-17 enhances recombinant protein production of CHO cells

artículo científico publicado en 2014

Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA ⬇️

artículo científico publicado el 1 de enero de 2012

Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5.

artículo científico publicado en 2007

The broadly neutralizing anti-human immunodeficiency virus type 1 4E10 monoclonal antibody is better adapted to membrane-bound epitope recognition and blocking than 2F5.

artículo científico publicado en 2008

The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5.

artículo científico publicado en 2004

Topological transformation of liposomes by a membrane-affecting domain of recombinant human erythropoietin

artículo científico publicado en 2010

Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis.

artículo científico

Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties

scientific article published on 12 March 2020

Upstream and downstream processing of recombinant IgA.

artículo científico

Validated method for quantification of genetically modified organisms in samples of maize flour.

artículo científico publicado en 2006

Lista de obras de Renate Kunert

A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples

A novel strategy for quantitative isoform detection directly performed from culture supernatant.

A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits

A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1.

A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells

A study on the temperature dependency and time course of the cold capture antibody secretion assay

Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

Advances in recombinant antibody manufacturing

Analysis of changes during subclone development and ageing of human antibody-producing heterohybridoma cells by northern blot and flow cytometry.

Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides

Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1.

Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5

Anti-neuroblastoma effect of ch14.18 antibody produced in CHO cells is mediated by NK-cells in mice.

Antibody domain exchange is an immunological solution to carbohydrate cluster recognition

Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations

Applicability of different fluorescent dyes for isoform quantification on linear IPG gels

Benchmarking of commercially available CHO cell culture media for antibody production.

Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changes.

Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells.

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression.

CHO-recombinant human growth hormone as a protease sensitive reporter protein.

Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies.

Ch14.18 antibody produced in CHO cells in relapsed or refractory Stage 4 neuroblastoma patients: a SIOPEN Phase 1 study.

Changes during subclone development and ageing of human antibody-producing recombinant CHO cells

Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters.

Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12.

Characterization of molecular features, antigen-binding, and in vitro properties of IgG and IgM variants of 4E10, an anti-HIV type 1 neutralizing monoclonal antibody.

Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression

Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies

Cross-Neutralizing Human Monoclonal Anti-HIV-1 Antibody 2F5: Preparation and Crystallographic Analysis of the Free and Epitope-Complexed Forms of its F ab Fragment

Crystal structure of the complex between the F(ab)' fragment of the cross-neutralizing anti-HIV-1 antibody 2F5 and the F(ab) fragment of its anti-idiotypic antibody 3H6

Differential gene expression of a feed-spiked super-producing CHO cell line

Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line

Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12.

Engineering of Pichia pastoris for improved production of antibody fragments.

Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting

Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

Evaluation of the potency of the anti-idiotypic antibody Ab2/3H6 mimicking gp41 as an HIV-1 vaccine in a rabbit prime/boost study

Exploration of BAC versus plasmid expression vectors in recombinant CHO cells.

Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis

Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12.

Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures.

Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients.

Glycan profile of CHO derived IgM purified by highly efficient single step affinity chromatography.

Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth

Heterologous protein production using euchromatin-containing expression vectors in mammalian cells

High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris

Humanization strategies for an anti-idiotypic antibody mimicking HIV-1 gp41 ⬇️

Identification of bottlenecks in antibody expression using targeted gene integration.

Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation

Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH.

IgM characterization directly performed in crude culture supernatants by a new simple electrophoretic method.

Impact of temperature and pH on recombinant human IgM quality attributes and productivity

Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile

Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

In planta protein sialylation through overexpression of the respective mammalian pathway

In search of expression bottlenecks in recombinant CHO cell lines--a case study.

Inefficient CAR-proximal signaling blunts antigen sensitivity

Influence of cell culture media and feed supplements on cell metabolism and quality of IgG produced in CHO-K1, CHO-S, and CHO-DG44.

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

Inhibition of human immunodeficiency virus type 1 entry in cells expressing gp41-derived peptides

Inhibition of in vivo HIV infection in humanized mice by gene therapy of human hematopoietic stem cells with a lentiviral vector encoding a broadly neutralizing anti-HIV antibody

Interaction of anti-HIV type 1 antibody 2F5 with phospholipid bilayers and its relevance for the mechanism of virus neutralization

Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM

Lessons learned from merging wet lab experiments with molecular simulation to improve mAb humanization

Membrane association and epitope recognition by HIV-1 neutralizing anti-gp41 2F5 and 4E10 antibodies.

Molecular Characterization of Five Neutralizing Anti-HIV Type 1 Antibodies: Identification of Nonconventional D Segments in the Human Monoclonal Antibodies 2G12 and 2F5

Molecular Simulations to Rationalize Humanized Ab2/3H6 Activity

Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control

Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering

Nomenclature of humanized mAbs: Early concepts, current challenges and future perspectives

Novel bifunctional single-chain variable antibody fragments to enhance virolysis by complement: generation and proof-of-concept

Novel human renal proximal tubular cell line for the production of complex proteins.

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors

Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells

Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern