Lista de obras - Zbynek Prokop - Dominio Público Uruguay

A Haloalkane Dehalogenase from a Marine Microbial Consortium Possessing Exceptionally Broad Substrate Specificity.

artículo científico publicado en 2017

An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation

artículo científico publicado en 2020

Bioavailability and mobility of organic contaminants in soil: new three-step ecotoxicological evaluation.

artículo científico publicado en 2015

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

artículo científico publicado en 2010

Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

artículo científico publicado en 2009

CalFitter: a web server for analysis of protein thermal denaturation data.

artículo científico publicado en 2018

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling

artículo científico publicado en 2017

Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

artículo científico publicado en 2003

Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases

artículo científico publicado en 2005

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2.

artículo científico publicado en 2017

Computer-assisted engineering of the synthetic pathway for biodegradation of a toxic persistent pollutant.

artículo científico publicado en 2013

Conformational changes allow processing of bulky substrates by a haloalkane dehalogenase with a small and buried active site

artículo científico publicado en 2018

Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics

artículo científico publicado en 2019

Degradation of beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26

artículo científico publicado en 2005

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips ⬇️

scientific article published on 23 September 2018

Determination of capsidiol in tobacco cells culture by HPLC.

artículo científico publicado en 2010

Development of Fluorescent Assay for Monitoring of Dehalogenase Activity

artículo científico publicado en 2018

Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites.

artículo científico publicado en 2017

Discovery of Novel Haloalkane Dehalogenase Inhibitors.

artículo científico publicado en 2016

Dynamics and hydration explain failed functional transformation in dehalogenase design

artículo científico publicado en 2014

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

artículo científico publicado en 2013

Enzyme Tunnels and Gates As Relevant Targets in Drug Design.

artículo científico publicado en 2016

Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

artículo científico publicado en 2016

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

artículo científico publicado en 2015

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents

artículo científico publicado en 2013

Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating.

artículo científico publicado en 2017

Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition

artículo científico publicado en 2002

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

artículo científico publicado en 2015

FireProt: web server for automated design of thermostable proteins

artículo científico publicado en 2017

Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application

artículo científico publicado en 2015

Halide-stabilizing residues of haloalkane dehalogenases studied by quantum mechanic calculations and site-directed mutagenesis

artículo científico publicado en 2002

Haloalkane Dehalogenases From Marine Organisms

artículo científico publicado en 2018

Haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26: X-ray crystallographic studies of dehalogenation of brominated substrates

artículo científico publicado en 2003

Haloalkane dehalogenases: biotechnological applications.

artículo científico

Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane

artículo científico publicado en 2014

Impact of the access tunnel engineering on catalysis is strictly ligand-specific

artículo científico publicado en 2018

Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets.

artículo científico publicado en 2014

Kinetics of binding of fluorescent ligands to enzymes with engineered access tunnels.

artículo científico publicado en 2016

Maximizing the efficiency of multienzyme process by stoichiometry optimization.

artículo científico publicado en 2014

Mechanism-based discovery of novel substrates of haloalkane dehalogenases using in silico screening

artículo científico publicado en 2014

Metagenome-derived haloalkane dehalogenases with novel catalytic properties

artículo científico publicado en 2017

Microscopic monitoring provides information on structure and properties during biocatalyst immobilization

artículo científico publicado en 2014

Mobility and toxicity of metals in sandy sediments deposited on land

artículo científico publicado en 2003

Mobility, bioavailability, and toxic effects of cadmium in soil samples.

artículo científico publicado en 2003

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

artículo científico publicado en 2003

Molecular Gating of an Engineered Enzyme Captured in Real Time

artículo científico publicado en 2018

Novel LinA type 3 δ-hexachlorocyclohexane dehydrochlorinase.

artículo científico publicado en 2015

Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil.

artículo científico publicado en 2012

Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.

artículo científico publicado en 2009

Perspectives: biotechnology in Czech Republic, the past and the future.

artículo científico publicado en 2006

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer.

artículo científico publicado en 2017

Quantitative structure–activity relationships for toxicity and genotoxicity of halogenated aliphatic compounds: Wing spot test of Drosophila melanogaster

artículo científico publicado en 2007

Reconstruction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB

scientific journal article

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

artículo científico publicado en 2009

Regio- and Enantioselective Sequential Dehalogenation of rac-1,3-Dibromobutane by Haloalkane Dehalogenase LinB.

artículo científico publicado en 2016

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

artículo científico publicado en 2013

Sensitive operation of enzyme-based biodevices by advanced signal processing.

artículo científico publicado en 2018

Sphingobium baderi sp. nov., isolated from a hexachlorocyclohexane dump site.

artículo científico publicado en 2012

Sphingobium czechense sp. nov., isolated from a hexachlorocyclohexane dump site.

artículo científico publicado en 2012

Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexane.

artículo científico publicado en 2014

Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature ⬇️

artículo científico publicado el 27 de junio de 2011

Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites

artículo científico publicado en 2014

Substrate inhibition by the blockage of product release and its control by tunnel engineering

artículo científico publicado en 2021

The effect of a unique halide-stabilizing residue on the catalytic properties of haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58.

artículo científico publicado en 2013

The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution

artículo científico publicado en 2006

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry

artículo científico publicado en 2007

Lista de obras de Zbynek Prokop

A Haloalkane Dehalogenase from a Marine Microbial Consortium Possessing Exceptionally Broad Substrate Specificity.

An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation

Bioavailability and mobility of organic contaminants in soil: new three-step ecotoxicological evaluation.

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

CalFitter: a web server for analysis of protein thermal denaturation data.

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling

Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2.

Computer-assisted engineering of the synthetic pathway for biodegradation of a toxic persistent pollutant.

Conformational changes allow processing of bulky substrates by a haloalkane dehalogenase with a small and buried active site

Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics

Degradation of beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips ⬇️

Determination of capsidiol in tobacco cells culture by HPLC.

Development of Fluorescent Assay for Monitoring of Dehalogenase Activity

Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites.

Discovery of Novel Haloalkane Dehalogenase Inhibitors.

Dynamics and hydration explain failed functional transformation in dehalogenase design

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

Enzyme Tunnels and Gates As Relevant Targets in Drug Design.

Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents

Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating.

Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

FireProt: web server for automated design of thermostable proteins

Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application

Halide-stabilizing residues of haloalkane dehalogenases studied by quantum mechanic calculations and site-directed mutagenesis

Haloalkane Dehalogenases From Marine Organisms

Haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26: X-ray crystallographic studies of dehalogenation of brominated substrates

Haloalkane dehalogenases: biotechnological applications.

Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane

Impact of the access tunnel engineering on catalysis is strictly ligand-specific

Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets.

Kinetics of binding of fluorescent ligands to enzymes with engineered access tunnels.

Maximizing the efficiency of multienzyme process by stoichiometry optimization.

Mechanism-based discovery of novel substrates of haloalkane dehalogenases using in silico screening

Metagenome-derived haloalkane dehalogenases with novel catalytic properties

Microscopic monitoring provides information on structure and properties during biocatalyst immobilization

Mobility and toxicity of metals in sandy sediments deposited on land

Mobility, bioavailability, and toxic effects of cadmium in soil samples.

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

Molecular Gating of an Engineered Enzyme Captured in Real Time

Novel LinA type 3 δ-hexachlorocyclohexane dehydrochlorinase.

Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil.

Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.

Perspectives: biotechnology in Czech Republic, the past and the future.

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer.

Quantitative structure–activity relationships for toxicity and genotoxicity of halogenated aliphatic compounds: Wing spot test of Drosophila melanogaster

Reconstruction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

Regio- and Enantioselective Sequential Dehalogenation of rac-1,3-Dibromobutane by Haloalkane Dehalogenase LinB.

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

Sensitive operation of enzyme-based biodevices by advanced signal processing.

Sphingobium baderi sp. nov., isolated from a hexachlorocyclohexane dump site.

Sphingobium czechense sp. nov., isolated from a hexachlorocyclohexane dump site.

Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexane.

Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature ⬇️

Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites

Substrate inhibition by the blockage of product release and its control by tunnel engineering

The effect of a unique halide-stabilizing residue on the catalytic properties of haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58.

The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry