Lista de obras - Frank Bernhard - Dominio Público Uruguay

A Disulfide Bridge Network within the Soluble Periplasmic Domain Determines Structure and Function of the Outer Membrane Protein RCSF

artículo científico publicado en 2011

A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.

artículo científico publicado en 1993

A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.

artículo científico publicado en 2006

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

artículo científico publicado en 2012

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

article

Analysis of engineered multifunctional peptide synthetases. Enzymatic characterization of surfactin synthetase domains in hybrid bimodular systems

artículo científico publicado en 1999

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

artículo científico publicado en 2017

Artificial environments for the co-translational stabilization of cell-free expressed proteins

artículo científico publicado en 2013

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain

artículo científico publicado en 2004

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing

artículo científico publicado en 2015

Cell free expression and functional reconstitution of eukaryotic drug transporters

scientific journal article

Cell-Free Expression of G-Protein-Coupled Receptors

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

artículo científico publicado en 2016

Cell-free expression and assembly of ATP synthase.

artículo científico publicado en 2011

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination

artículo científico publicado en 2014

Cell-free expression and stable isotope labelling strategies for membrane proteins

Cell-free expression as an emerging technique for the large scale production of integral membrane protein

artículo científico publicado en 2006

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

artículo científico

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening

artículo científico publicado en 2012

Cell-free expression profiling of E. coli inner membrane proteins

artículo científico publicado en 2010

Cell-free expression--making a mark.

artículo científico

Cell-free production of G protein-coupled receptors for functional and structural studies.

artículo científico publicado en 2007

Cell-free production of integral membrane proteins on a preparative scale

artículo científico publicado en 2007

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

artículo científico publicado en 2017

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

artículo científico publicado en 2012

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

artículo científico publicado en 2012

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments

artículo científico publicado en 2016

Co-translational stabilization of insoluble proteins in cell-free expression systems

artículo científico publicado en 2015

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins

artículo científico publicado en 2006

Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment

artículo científico publicado en 2012

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression

artículo científico publicado en 2016

Conformational Switches Modulate Protein Interactions in Peptide Antibiotic Synthetases

scientific article published in Science

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.

artículo científico publicado en 2013

Cryo-EM structure of cell-free synthesized human histamine 2 receptor/Gs complex in nanodisc environment

artículo científico publicado en 2024

Efficient Strategy for the Rapid Backbone Assignment of Membrane Proteins

artículo científico publicado en 2005

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

artículo científico publicado en 2005

From Gene to Function: Cell-free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs

artículo científico publicado en 2017

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins

artículo científico publicado en 2016

Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation

article

Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism ⬇️

artículo científico publicado en 2012

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification

artículo científico publicado en 2011

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

artículo científico publicado en 2013

Genetic transfer of amylovoran and stewartan synthesis between Erwinia amylovora and Erwinia stewartii

scientific article published on 01 May 1996

High level cell-free expression and specific labeling of integral membrane proteins

artículo científico publicado en 2004

High-level cell-free production of membrane proteins with nanodiscs.

artículo científico publicado en 2014

Inhibition of microsomal prostaglandin E2 synthase-1 as a molecular basis for the anti-inflammatory actions of boswellic acids from frankincense ⬇️

artículo científico publicado en 2011

Insights into Cotranslational Membrane Protein Insertion by Combined LILBID-Mass Spectrometry and NMR Spectroscopy

artículo científico publicado en 2017

Interaction of the regulator proteins RcsA and RcsB with the promoter of the operon for amylovoran biosynthesis in Erwinia amylovora.

artículo científico publicado en 1997

Labeling of membrane proteins by cell-free expression

artículo científico

Large-scale production of functional membrane proteins

artículo científico publicado en 2008

Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism

artículo científico publicado en 2018

Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway

artículo científico publicado en 2015

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs.

artículo científico publicado en 2017

Membrane protein expression in cell-free systems

artículo científico publicado en 2010

Membrane protein production in Escherichia coli cell-free lysates

artículo científico publicado en 2015

Modification of biologically active peptides: production of a novel lipohexapeptide after engineering of Bacillus subtilis surfactin synthetase.

artículo científico publicado en 2002

Modified lipid and protein dynamics in nanodiscs

artículo científico publicado en 2012

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

article published in 2010

Modulation of the Rcs-mediated signal transfer by conformational flexibility.

artículo científico publicado en 2008

Molecular engineering aspects for the production of new and modified biosurfactants.

artículo científico publicado en 2010

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.

artículo científico publicado en 2006

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins

artículo científico publicado en 2018

Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies

artículo científico publicado en 2007

Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes

artículo científico publicado en 2011

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

artículo científico publicado en 2007

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes

artículo científico publicado en 2010

Production of membrane proteins using cell-free expression systems

artículo científico publicado en 2008

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs

artículo científico publicado en 2015

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

artículo científico publicado en 2009

Single-Molecule Force Spectroscopy from Nanodiscs: An Assay to Quantify Folding, Stability, and Interactions of Native Membrane Proteins

artículo científico publicado en 2011

Solution NMR Structure of Proteorhodopsin

artículo científico publicado en 2011

Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.

artículo científico publicado en 2004

Strategies for the Cell-Free Expression of Membrane Proteins

Structural Analysis of the DNA-binding Domain of theErwinia amylovora RcsB Protein and Its Interaction with the RcsAB Box ⬇️

artículo científico publicado el 5 de marzo de 2003

Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex

artículo científico publicado en 2011

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase

artículo científico publicado en 2008

Structural investigation of the C-terminal catalytic fragment of presenilin 1

artículo científico publicado en 2010

Substrate specificity of hybrid modules from peptide synthetases.

artículo científico publicado en 1997

Systematic optimization of cell-free synthesized human endothelin B receptor folding

artículo científico publicado en 2018

Systems for the cell-free synthesis of proteins.

artículo científico publicado en 2012

The E. coli S30 lysate proteome: A prototype for cell-free protein production

artículo científico publicado en 2017

The large extracellular loop of organic cation transporter 1 influences substrate affinity and is pivotal for oligomerization

scientific journal article

The molecular pharmacology and in vivo activity of 2-(4-chloro-6-(2,3-dimethylphenylamino)pyrimidin-2-ylthio)octanoic acid (YS121), a dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase

artículo científico publicado en 2009

The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase.

artículo científico publicado en 1999

The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6.

artículo científico publicado en 2017

The synaptic vesicle protein SV31 assembles into a dimer and transports Zn2.

artículo científico publicado en 2016

Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins

artículo científico publicado en 2008

Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues.

artículo científico publicado en 2005

Lista de obras de Frank Bernhard

A Disulfide Bridge Network within the Soluble Periplasmic Domain Determines Structure and Function of the Outer Membrane Protein RCSF

A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.

A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

Analysis of engineered multifunctional peptide synthetases. Enzymatic characterization of surfactin synthetase domains in hybrid bimodular systems

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

Artificial environments for the co-translational stabilization of cell-free expressed proteins

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing

Cell free expression and functional reconstitution of eukaryotic drug transporters

Cell-Free Expression of G-Protein-Coupled Receptors

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

Cell-free expression and assembly of ATP synthase.

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination

Cell-free expression and stable isotope labelling strategies for membrane proteins

Cell-free expression as an emerging technique for the large scale production of integral membrane protein

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening

Cell-free expression profiling of E. coli inner membrane proteins

Cell-free expression--making a mark.

Cell-free production of G protein-coupled receptors for functional and structural studies.

Cell-free production of integral membrane proteins on a preparative scale

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments

Co-translational stabilization of insoluble proteins in cell-free expression systems

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins

Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression

Conformational Switches Modulate Protein Interactions in Peptide Antibiotic Synthetases

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.

Cryo-EM structure of cell-free synthesized human histamine 2 receptor/Gs complex in nanodisc environment

Efficient Strategy for the Rapid Backbone Assignment of Membrane Proteins

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

From Gene to Function: Cell-free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins

Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation

Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism ⬇️

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

Genetic transfer of amylovoran and stewartan synthesis between Erwinia amylovora and Erwinia stewartii

High level cell-free expression and specific labeling of integral membrane proteins

High-level cell-free production of membrane proteins with nanodiscs.

Inhibition of microsomal prostaglandin E2 synthase-1 as a molecular basis for the anti-inflammatory actions of boswellic acids from frankincense ⬇️

Insights into Cotranslational Membrane Protein Insertion by Combined LILBID-Mass Spectrometry and NMR Spectroscopy

Interaction of the regulator proteins RcsA and RcsB with the promoter of the operon for amylovoran biosynthesis in Erwinia amylovora.

Labeling of membrane proteins by cell-free expression

Large-scale production of functional membrane proteins

Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism

Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs.

Membrane protein expression in cell-free systems

Membrane protein production in Escherichia coli cell-free lysates

Modification of biologically active peptides: production of a novel lipohexapeptide after engineering of Bacillus subtilis surfactin synthetase.

Modified lipid and protein dynamics in nanodiscs

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

Modulation of the Rcs-mediated signal transfer by conformational flexibility.

Molecular engineering aspects for the production of new and modified biosurfactants.

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins

Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies

Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes

Production of membrane proteins using cell-free expression systems

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

Single-Molecule Force Spectroscopy from Nanodiscs: An Assay to Quantify Folding, Stability, and Interactions of Native Membrane Proteins

Solution NMR Structure of Proteorhodopsin

Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.

Strategies for the Cell-Free Expression of Membrane Proteins

Structural Analysis of the DNA-binding Domain of theErwinia amylovora RcsB Protein and Its Interaction with the RcsAB Box ⬇️

Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase

Structural investigation of the C-terminal catalytic fragment of presenilin 1

Substrate specificity of hybrid modules from peptide synthetases.

Systematic optimization of cell-free synthesized human endothelin B receptor folding