Filtros de búsqueda

Lista de obras de Duarte Miguel F Prazeres

A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles

artículo científico publicado en 2017

A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (r)-beta-hydroxyisobutyric acid.

artículo científico publicado en 2001

Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.

artículo científico publicado en 2008

Alternatives for the intermediate recovery of plasmid DNA: performance, economic viability and environmental impact.

artículo científico publicado en 2009

Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events

artículo científico publicado en 2010

Anion exchange purification of plasmid DNA using expanded bed adsorption.

artículo científico publicado en 2000

Application of central composite design for DNA hybridization onto magnetic microparticles

artículo científico publicado en 2009

Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography.

artículo científico publicado en 2003

Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification.

artículo científico publicado en 2009

Biotransformation in organic media by enzymes and whole cells

article

Biotransformations in two-liquid-phase systems

Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains.

artículo científico publicado en 2014

Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography.

artículo científico publicado en 2010

Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas

Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles

artículo científico publicado en 2006

Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples.

artículo científico publicado en 2008

Chromatography of plasmid DNA.

artículo científico publicado en 2005

Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography

artículo científico publicado en 2007

Colorimetric Detection of DNA Strands on Cellulose Microparticles Using ZZ-CBM Fusions and Gold Nanoparticles

artículo científico publicado en 2019

Colorimetric detection of D-dimer in a paper-based immunodetection device.

artículo científico publicado en 2017

Comparative analysis of antigen-targeting sequences used in DNA vaccines.

artículo científico publicado en 2010

Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products.

artículo científico publicado en 2003

DNA vaccines: a rational design against parasitic diseases.

artículo científico publicado en 2010

De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production

artículo científico publicado en 2012

Density Gradient Selection of Colloidal Silver Nanotriangles for Assembling Dye-Particle Plasmophores

scientific article published on 18 June 2019

Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors

artículo científico publicado en 2008

Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes

article by Pedro Novo et al published January 2013 in Sensors and Actuators B

Development of a candidate DNA vaccine against Maedi-Visna virus.

artículo científico publicado en 2007

Development of a nicking endonuclease-assisted method for the purification of minicircles.

artículo científico publicado en 2016

Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection.

artículo científico publicado en 2014

Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery

artículo científico publicado en 2012

Dipeptide synthesis and separation in a reversed micellar membrane reactor

scientific article published on 01 December 1994

Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography.

artículo científico publicado en 2007

Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.

artículo científico publicado en 2009

Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips

scientific article published on 09 August 2005

Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: simplex optimization of the biotransformation

Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges

artículo científico publicado en 2014

Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor-Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy

scientific article published on 13 November 2018

Enhancement of DNA vaccine efficacy by intracellular targeting strategies

artículo científico publicado en 2014

Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies.

artículo científico publicado en 2009

Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin

artículo científico publicado en 2010

Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters

artículo científico publicado en 2014

Extreme Enhancement of Single-Molecule Fluorescence from Porphyrins Induced by Gold Nanodimer Antennas

artículo científico publicado en 2019

Fluorescence correlation spectroscopy study of the complexation of DNA hybrids, IgG antibody, and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module.

artículo científico publicado en 2017

Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement

artículo científico publicado en 2020

G protein-coupled receptors: an overview of signaling mechanisms and screening assays

artículo científico

Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes

artículo científico publicado el 15 de febrero de 2011

High frequency plasmid recombination mediated by 28 bp direct repeats

artículo científico publicado en 2008

Horseradish peroxidase: a valuable tool in biotechnology

artículo científico publicado en 2003

Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B

scientific article published on 01 July 2003

Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory.

artículo científico publicado en 2002

Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization

artículo científico publicado en 2010

Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays

artículo científico publicado en 2004

Impact of plasmid quality on lipoplex-mediated transfection

artículo científico publicado en 2013

Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers

artículo científico publicado en 2013

Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase

artículo científico publicado en 2016

Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors

artículo científico publicado en 2012

Ionic Liquid-Polymer Nanoparticle Hybrid Systems as New Tools to Deliver Poorly Soluble Drugs

artículo científico publicado en 2019

Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics

artículo científico publicado en 2013

Microbial Stereoselective Oxidation of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric Acid in Aqueous/organic Biphasic Systems

article

Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes.

artículo científico publicado en 2011

Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters

artículo científico publicado en 2013

Mutation detection in plasmid-based biopharmaceuticals.

artículo científico publicado en 2011

On the dual effect of glucose during production of pBAD/AraC-based minicircles

artículo científico publicado en 2014

On the stability of plasmid DNA vectors during cell culture and purification.

artículo científico publicado en 2007

Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA.

artículo científico publicado en 2006

Optimization of the primary recovery of human interferon alpha2b from Escherichia coli inclusion bodies.

artículo científico publicado en 2005

Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site

scientific article published on 20 May 2019

Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing

artículo científico publicado en 2014

Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase

Prediction of diffusion coefficients of plasmids

artículo científico publicado en 2008

Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process

scientific article published on 03 July 2007

Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography

artículo científico publicado en 1998

Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography

artículo científico publicado en 2018

Production, purification and analysis of an experimental DNA vaccine against rabies.

artículo científico publicado en 2001

Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition.

artículo científico publicado en 2012

Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography.

artículo científico publicado en 2011

Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography.

artículo científico publicado en 2008

Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography

artículo científico publicado en 2005

Purification of plasmids for gene therapy and DNA vaccination.

artículo científico publicado en 2001

Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma

artículo científico publicado en 2018

Recombination frequency in plasmid DNA containing direct repeats--predictive correlation with repeat and intervening sequence length

artículo científico publicado en 2008

Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-agarose chromatography step

artículo científico publicado en 2006

Separation and Analysis of Plasmid Denatured Forms Using Hydrophobic Interaction Chromatography

artículo científico publicado en 1999

Separation of plasmid DNA topoisomers by multimodal chromatography.

artículo científico publicado en 2016

Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support

artículo científico publicado en 2005

Single base mismatch detection by microsecond voltage pulses

artículo científico publicado en 2005

Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography

artículo científico publicado en 2007

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C.

artículo científico publicado en 2017

Stability of free and immobilised peroxidase in aqueous–organic solvents mixtures

Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices

artículo científico publicado en 2009

Structural instability of plasmid biopharmaceuticals: challenges and implications.

artículo científico publicado en 2009

Studies on the Batch Adsorption of Plasmid DNA onto Anion-Exchange Chromatographic Supports

artículo científico publicado en 2000

Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads.

artículo científico publicado en 2011

The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA

artículo científico publicado en 2008

The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases

artículo científico publicado en 2004

The role of probe–probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles

artículo científico publicado en 2009

Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR.

artículo científico publicado en 2007

Towards effective non-viral gene delivery vector.

artículo científico publicado en 2015

Towards the miniaturization of GPCR-based live-cell screening assays.

artículo científico

Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis

artículo científico publicado en 2009

Translational features of human alpha 2b interferon production in Escherichia coli.

artículo científico publicado en 2004

Trends in dengue diagnosis.

artículo científico publicado en 2005

Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.

artículo científico publicado en 2010

Use of ImageJ to recover information from individual cells in a G protein-coupled receptor assay

artículo científico publicado en 2015

Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography.

artículo científico publicado en 2012