Lista de obras - Warren Wakarchuk - Dominio Público Uruguay

A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans

artículo científico publicado en 2018

A Campylobacter jejuni gene associated with immune-mediated neuropathy

article

A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis.

artículo científico publicado en 2008

A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

artículo científico publicado en 2013

A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase

artículo científico publicado en 2009

A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis

artículo científico publicado en 2013

A sensitive fluorescence-based assay for monitoring GM2 ganglioside hydrolysis in live patient cells and their lysates

artículo científico publicado en 2009

A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni

artículo científico publicado en 2004

Allylmalonamide as a bivalent linker: synthesis of biantennary GM3-saccharide--keyhole limpet hemocyanin glycoconjugate and the immune response in mice

artículo científico publicado en 1999

Alternative donor substrates for inverting and retaining glycosyltransferases

artículo científico publicado en 2006

Amino acid sequence and thermostability of xylanase A from Schizophyllum commune

artículo científico publicado en 1993

Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses

artículo científico publicado en 2005

Analysis of bacterial glycolipids by capillary electrophoresis-electrospray mass spectrometry: Haemophilus influenzae and Neisseria meningitidis lipopolysaccharides

artículo científico publicado en 2003

Biochemical characterization of a polysialyltransferase from Mannheimia haemolytica A2 and comparison to other bacterial polysialyltransferases.

artículo científico publicado en 2013

Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis.

artículo científico publicado en 2000

Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases

artículo científico publicado en 2012

Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.

artículo científico publicado en 1996

Characterization of a recombinant Neisseria meningitidis alpha-2,3-sialyltransferase and its acceptor specificity

artículo científico publicado en 1997

Characterization of five β-glycoside hydrolases from Cellulomonas fimi ATCC 484.

artículo científico publicado en 2014

Characterization of the alpha-1,2-N-acetylglucosaminyltransferase of Neisseria gonorrhoeae, a key control point in lipooligosaccharide biosynthesis.

artículo científico publicado en 2004

Characterization of the alpha-2,8-polysialyltransferase from Neisseria meningitidis with synthetic acceptors, and the development of a self-priming polysialyltransferase fusion enzyme

artículo científico publicado en 2007

Chemical and chemoenzymatic synthesis of S-linked ganglioside analogues and their protein conjugates for use as immunogens

artículo científico publicado en 2006

Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces

artículo científico publicado en 2008

Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.

artículo científico publicado en 2005

Cloning of the lipooligosaccharide alpha-2,3-sialyltransferase from the bacterial pathogens Neisseria meningitidis and Neisseria gonorrhoeae.

artículo científico publicado en 1996

Comparison of α2,6-sialyltransferases for sialylation of therapeutic proteins

scientific article published on 01 September 2019

Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida.

artículo científico publicado en 2008

Construction of a hybrid β-hexosaminidase subunit capable of forming stable homodimers that hydrolyze GM2 ganglioside in vivo

artículo científico publicado en 2016

Development of an on-line preconcentration method for the analysis of pathogenic lipopolysaccharides using capillary electrophoresis-electrospray mass spectrometry. Application to small colony isolates

artículo científico publicado en 1998

Directed evolution of bacterial polysialyltransferases

artículo científico publicado en 2019

Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens

artículo científico publicado en 2012

Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.

artículo científico publicado en 1996

Enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid

artículo científico publicado en 2010

Evidence for acquisition of the lipooligosaccharide biosynthesis locus in Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, by horizontal exchange

artículo científico publicado en 2004

Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6

scientific article published on 01 June 2000

FlaA1, a new bifunctional UDP-GlcNAc C6 Dehydratase/ C4 reductase from Helicobacter pylori

artículo científico publicado en 2000

Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases.

artículo científico publicado en 2010

Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways.

artículo científico publicado en 2005

Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis

scientific article published on 01 August 1996

Genetics and molecular specificity of sialylation of Histophilus somni lipooligosaccharide (LOS) and the effect of LOS sialylation on Toll-like receptor-4 signaling ⬇️

artículo científico publicado el 5 de marzo de 2011

Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli

scientific article published on 26 March 2019

Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases

scientific article published on 01 December 1999

High-throughput screening methodology for the directed evolution of glycosyltransferases

artículo científico publicado en 2006

Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase.

artículo científico publicado en 2005

Identification and characterization of a lipopolysaccharide α,2,3-sialyltransferase from the human pathogen Helicobacter bizzozeronii

artículo científico publicado en 2012

Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid

artículo científico publicado en 2006

Identification of a lipopolysaccharide alpha-2,3-sialyltransferase from Haemophilus influenzae.

artículo científico publicado en 2001

In cellulo examination of a beta-alpha hybrid construct of beta-hexosaminidase A subunits, reported to interact with the GM2 activator protein and hydrolyze GM2 ganglioside

artículo científico publicado en 2013

Incorporation of N-Acetylneuraminic Acid into Haemophilus somnus Lipooligosaccharide (LOS): Enhancement of Resistance to Serum and Reduction of LOS Antibody Binding.

artículo científico publicado en 2002

Incorporation of N-acetylneuraminic acid into Haemophilus somnus lipooligosaccharide (LOS): enhancement of resistance to serum and reduction of LOS antibody binding.

artículo científico publicado en 2002

Inhibition of galactosyltransferases by a novel class of donor analogues

artículo científico publicado en 2012

Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue

artículo científico publicado en 2004

Large-scale in vivo synthesis of the carbohydrate moieties of gangliosides GM1 and GM2 by metabolically engineered Escherichia coli

artículo científico publicado en 2003

Mammalian sialyltransferases allow efficient <i>Escherichia coli</i>-based production of mucin-type O-glycoproteins but can also transfer Kdo

artículo científico publicado en 2021

Mechanism of stabilization of Bacillus circulans xylanase upon the introduction of disulfide bonds

artículo científico publicado en 2006

Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6.

artículo científico publicado en 2009

Mechanistic studies of a retaining alpha-galactosyltransferase from Neisseria meningitidis.

artículo científico publicado en 2002

NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base

artículo científico publicado en 2009

Novel biosynthetic functions of lipopolysaccharide rfaJ homologs from Helicobacter pylori

artículo científico publicado en 2005

One step closer to a sweet conclusion.

artículo científico publicado en 2002

Overexpression of the Bacillus subtilis and circulans xylanases in Escherichia coli.

artículo científico publicado en 1993

Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison

artículo científico publicado en 2000

Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase

artículo científico publicado en 1997

Preparative-scale chemoenzymatic synthesis of large carbohydrate assemblies using alpha (1-->4)-galactosyltransferase/UDP-4'-Gal-epimerase fusion protein

artículo científico publicado en 2003

Proteomic Analysis of the Secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482.

artículo científico publicado en 2016

Recognition of protein-linked glycans as a determinant of peptidase activity

artículo científico publicado en 2017

Recognition of sialylated poly-N-acetyllactosamine chains on N- and O-linked glycans by human and avian influenza A virus hemagglutinins.

artículo científico publicado en 2012

Review of phosphocholine substituents on bacterial pathogen glycans: synthesis, structures and interactions with host proteins.

artículo científico

Sialyltransferases with enhanced legionaminic acid transferase activity for the preparation of analogs of sialoglycoconjugates

scientific article published on 03 April 2015

Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes

artículo científico publicado en 2011

Small molecules containing hetero-bicyclic ring systems compete with UDP-Glc for binding to WaaG glycosyltransferase

artículo científico publicado en 2012

Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms

artículo científico publicado en 2007

Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog

artículo científico publicado en 2004

Structural and Kinetic Analysis of Substrate Binding to the Sialyltransferase Cst-II from Campylobacter jejuni

artículo científico publicado en 2011

Structural and Kinetic Characterizations of the Polysialic Acid O-Acetyltransferase OatWY from Neisseria meningitidis ⬇️

artículo científico publicado en 2009

Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori

artículo científico publicado en 2006

Structure and Mechanism of the Lipooligosaccharide Sialyltransferase from Neisseria meningitidis

artículo científico publicado en 2011

Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation

artículo científico publicado en 2015

Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I.

artículo científico publicado en 2013

The genetic bases for the variation in the lipo-oligosaccharide of the mucosal pathogen, Campylobacter jejuni. Biosynthesis of sialylated ganglioside mimics in the core oligosaccharide

artículo científico publicado en 2001

The living factory: in vivo production of N-acetyllactosamine containing carbohydrates in E. coli

scientific article published on 01 March 1999

The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion

artículo científico publicado en 1998

Transition state analysis of Vibrio cholerae sialidase-catalyzed hydrolyses of natural substrate analogues

artículo científico publicado en 2012

Two GC-rich DNA elements ofChlamydomonas reinhardtii with complex arrangements of directly repeated sequence motifs ⬇️

artículo científico publicado el 1 de enero de 1992

Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis

artículo científico publicado en 2006

Variants of the beta 1,3-galactosyltransferase CgtB from the bacterium Campylobacter jejuni have distinct acceptor specificities

artículo científico publicado en 2007

X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid

artículo científico publicado en 2017

Lista de obras de Warren Wakarchuk

A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans

A Campylobacter jejuni gene associated with immune-mediated neuropathy

A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis.

A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase

A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis

A sensitive fluorescence-based assay for monitoring GM2 ganglioside hydrolysis in live patient cells and their lysates

A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni

Allylmalonamide as a bivalent linker: synthesis of biantennary GM3-saccharide--keyhole limpet hemocyanin glycoconjugate and the immune response in mice

Alternative donor substrates for inverting and retaining glycosyltransferases

Amino acid sequence and thermostability of xylanase A from Schizophyllum commune

Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses

Analysis of bacterial glycolipids by capillary electrophoresis-electrospray mass spectrometry: Haemophilus influenzae and Neisseria meningitidis lipopolysaccharides

Biochemical characterization of a polysialyltransferase from Mannheimia haemolytica A2 and comparison to other bacterial polysialyltransferases.

Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis.

Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases

Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.

Characterization of a recombinant Neisseria meningitidis alpha-2,3-sialyltransferase and its acceptor specificity

Characterization of five β-glycoside hydrolases from Cellulomonas fimi ATCC 484.

Characterization of the alpha-1,2-N-acetylglucosaminyltransferase of Neisseria gonorrhoeae, a key control point in lipooligosaccharide biosynthesis.

Characterization of the alpha-2,8-polysialyltransferase from Neisseria meningitidis with synthetic acceptors, and the development of a self-priming polysialyltransferase fusion enzyme

Chemical and chemoenzymatic synthesis of S-linked ganglioside analogues and their protein conjugates for use as immunogens

Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces

Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.

Cloning of the lipooligosaccharide alpha-2,3-sialyltransferase from the bacterial pathogens Neisseria meningitidis and Neisseria gonorrhoeae.

Comparison of α2,6-sialyltransferases for sialylation of therapeutic proteins

Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida.

Construction of a hybrid β-hexosaminidase subunit capable of forming stable homodimers that hydrolyze GM2 ganglioside in vivo

Development of an on-line preconcentration method for the analysis of pathogenic lipopolysaccharides using capillary electrophoresis-electrospray mass spectrometry. Application to small colony isolates

Directed evolution of bacterial polysialyltransferases

Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens

Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.

Enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid

Evidence for acquisition of the lipooligosaccharide biosynthesis locus in Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, by horizontal exchange

Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6

FlaA1, a new bifunctional UDP-GlcNAc C6 Dehydratase/ C4 reductase from Helicobacter pylori

Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases.

Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways.

Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis

Genetics and molecular specificity of sialylation of Histophilus somni lipooligosaccharide (LOS) and the effect of LOS sialylation on Toll-like receptor-4 signaling ⬇️

Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli

Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases

High-throughput screening methodology for the directed evolution of glycosyltransferases

Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase.

Identification and characterization of a lipopolysaccharide α,2,3-sialyltransferase from the human pathogen Helicobacter bizzozeronii

Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid

Identification of a lipopolysaccharide alpha-2,3-sialyltransferase from Haemophilus influenzae.

In cellulo examination of a beta-alpha hybrid construct of beta-hexosaminidase A subunits, reported to interact with the GM2 activator protein and hydrolyze GM2 ganglioside

Incorporation of N-Acetylneuraminic Acid into Haemophilus somnus Lipooligosaccharide (LOS): Enhancement of Resistance to Serum and Reduction of LOS Antibody Binding.

Incorporation of N-acetylneuraminic acid into Haemophilus somnus lipooligosaccharide (LOS): enhancement of resistance to serum and reduction of LOS antibody binding.

Inhibition of galactosyltransferases by a novel class of donor analogues

Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue

Large-scale in vivo synthesis of the carbohydrate moieties of gangliosides GM1 and GM2 by metabolically engineered Escherichia coli

Mammalian sialyltransferases allow efficient <i>Escherichia coli</i>-based production of mucin-type O-glycoproteins but can also transfer Kdo

Mechanism of stabilization of Bacillus circulans xylanase upon the introduction of disulfide bonds

Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6.

Mechanistic studies of a retaining alpha-galactosyltransferase from Neisseria meningitidis.

NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base

Novel biosynthetic functions of lipopolysaccharide rfaJ homologs from Helicobacter pylori

One step closer to a sweet conclusion.

Overexpression of the Bacillus subtilis and circulans xylanases in Escherichia coli.

Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison

Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase

Preparative-scale chemoenzymatic synthesis of large carbohydrate assemblies using alpha (1-->4)-galactosyltransferase/UDP-4'-Gal-epimerase fusion protein

Proteomic Analysis of the Secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482.

Recognition of protein-linked glycans as a determinant of peptidase activity

Recognition of sialylated poly-N-acetyllactosamine chains on N- and O-linked glycans by human and avian influenza A virus hemagglutinins.

Review of phosphocholine substituents on bacterial pathogen glycans: synthesis, structures and interactions with host proteins.

Sialyltransferases with enhanced legionaminic acid transferase activity for the preparation of analogs of sialoglycoconjugates

Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes

Small molecules containing hetero-bicyclic ring systems compete with UDP-Glc for binding to WaaG glycosyltransferase

Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms

Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog

Structural and Kinetic Analysis of Substrate Binding to the Sialyltransferase Cst-II from Campylobacter jejuni

Structural and Kinetic Characterizations of the Polysialic Acid O-Acetyltransferase OatWY from Neisseria meningitidis ⬇️

Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori

Structure and Mechanism of the Lipooligosaccharide Sialyltransferase from Neisseria meningitidis

Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation

Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I.