Lista de obras - Tim Dafforn - Dominio Público Uruguay

6-mer peptide selectively anneals to a pathogenic serpin conformation and blocks polymerization. Implications for the prevention of Z alpha(1)-antitrypsin-related cirrhosis

artículo científico publicado en 2001

A Kinetic Mechanism for the Polymerization of α1-Antitrypsin

scientific article published in Journal of Biological Chemistry

A cluster of familial Creutzfeldt-Jakob disease mutations recapitulate conserved residues in Doppel: a case of molecular mimicry?

artículo científico publicado en 2002

A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex.

artículo científico publicado en 2004

A general method for relieving substrate inhibition in lactate dehydrogenases

artículo científico publicado en 1999

A method for detergent-free isolation of membrane proteins in their local lipid environment. ⬇️

artículo científico publicado en 2016

A novel model and molecular therapy for Z alpha-1 antitrypsin deficiency.

artículo científico publicado en 2011

A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

scientific article published on 01 December 2018

A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain

artículo científico publicado en 1999

An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles.

artículo científico publicado en 2018

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

artículo científico publicado en 2019

Antithrombins Wibble and Wobble (T85M/K): archetypal conformational diseases with in vivo latent-transition, thrombosis, and heparin activation.

artículo científico publicado en 1998

Assembly pathway of a designed alpha-helical protein fiber.

artículo científico publicado en 2010

Automated High-Throughput Capillary Circular Dichroism and Intrinsic Fluorescence Spectroscopy for Rapid Determination of Protein Structure

artículo científico publicado en 2019

Breaking the 200 nm limit for routine flow linear dichroism measurements using UV synchrotron radiation.

artículo científico publicado en 2008

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum.

artículo científico publicado en 2016

Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila

artículo científico publicado en 2002

Characterizing the assembly of the Sup35 yeast prion fragment, GNNQQNY: structural changes accompany a fiber-to-crystal switch.

artículo científico publicado en 2010

Correlation of the enzyme activities of Bacillus stearothermophilus lactate dehydrogenase on three substrates with the results of molecular dynamics/energy minimization conformational searching

scientific article published on 01 October 1997

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.

artículo científico publicado en 2010

De novo design of Ln(III) coiled coils for imaging applications.

artículo científico publicado en 2014

Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroism.

artículo científico publicado en 2011

Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs

artículo científico publicado en 2014

Detergent-free purification of ABC (ATP-binding-cassette) transporters.

artículo científico publicado en 2014

Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle

artículo científico publicado en 2015

Drosophila necrotic mutations mirror disease-associated variants of human serpins

artículo científico publicado en 2003

Dye Aggregate-Mediated Self-Assembly of Bacteriophage Bioconjugates

artículo científico publicado en 2018

Encapsulated membrane proteins: A simplified system for molecular simulation.

artículo científico publicado en 2016

Expression, purification and crystallization of the cell-division protein YgfE from Escherichia coli.

artículo científico publicado en 2005

Flow linear dichroism of some prototypical proteins.

artículo científico publicado en 2009

Fluorescence detected linear dichroism spectroscopy: A selective and sensitive probe for fluorophores in flow-oriented systems.

artículo científico publicado en 2018

Folding and Membrane Insertion of the Pore-Forming Peptide Gramicidin Occur as a Concerted Process

scientific article published on 07 August 2008

FtsZ fiber bundling is triggered by a conformational change in bound GTP.

artículo científico publicado en 2004

FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP.

artículo científico publicado en 2007

Functional and biophysical analysis of the C-terminus of the CGRP-receptor; a family B GPCR.

artículo científico publicado en 2008

Functional domains of the human epididymal protease inhibitor, eppin

artículo científico publicado en 2008

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential.

artículo científico publicado en 2016

Golgi protein FAPP2 tubulates membranes

artículo científico publicado en 2009

Heteropolymerization of S, I, and Z alpha1-antitrypsin and liver cirrhosis.

artículo científico publicado en 1999

High-resolution, three-dimensional modeling of human leukocyte antigen class I structure and surface electrostatic potential reveals the molecular basis for alloantibody binding epitopes

artículo científico publicado en 2011

Identification and characterization of trimethylamine N-oxide (TMAO) demethylase and TMAO permease in Methylocella silvestris BL2.

artículo científico publicado en 2014

Identification of a 4-mer Peptide Inhibitor that Effectively Blocks the Polymerization of Pathogenic Z α1-Antitrypsin

artículo científico publicado en 2006

Identification of the primary collagen-binding surface on human glycoprotein VI by site-directed mutagenesis and by a blocking phage antibody

artículo científico publicado en 2003

Inhibiting polymerization: new therapeutic strategies for Z alpha1-antitrypsin-related emphysema

artículo científico publicado en 2004

Inhibitory activity of the Drosophila melanogaster serpin Necrotic is dependent on lysine residues in the D-helix

artículo científico publicado en 2006

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

artículo científico publicado en 2003

Kinetic study of the thermal denaturation of a hyperthermostable extracellular α-amylase from Pyrococcus furiosus.

artículo científico publicado en 2013

Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

scientific article published on 01 May 2019

Linear dichroism for the detection of single base pair mutationsElectronic supplementary information (ESI) available: Figures S1–S4. See http://www.rsc.org/suppdata/cc/b1/b107830p/

artículo científico publicado en 2001

Linear dichroism of biomolecules: which way is up?

artículo científico publicado en 2004

Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold

scientific article published on 20 August 2018

Location of auxilin within a clathrin cage.

artículo científico publicado en 2004

Looking at long molecules in solution: what happens when they are subjected to Couette flow?

artículo científico publicado en 2006

Membrane protein extraction and purification using styrene-maleic acid (SMA) copolymer: effect of variations in polymer structure.

artículo científico publicado en 2016

Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.

artículo científico publicado en 2016

Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer

artículo científico publicado en 2009

Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism

artículo científico publicado en 2004

Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis.

artículo científico publicado en 2006

Molecular studies of anti-HLA-A2 using light-chain shuffling: a structural model for HLA antibody binding.

artículo científico publicado en 2004

Multifunctional graphene oxide-bacteriophage based porous three-dimensional micro-nanocomposites

scientific article published on 04 July 2019

Murine serpin 2A is a redox-sensitive intracellular protein.

artículo científico publicado en 2003

Mutation of M13 Bacteriophage Major Coat Protein for Increased Conjugation to Exogenous Compounds

scientific article published on 01 June 2018

Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ

artículo científico publicado en 2019

Natively unfolded domains in endocytosis: hooks, lines and linkers.

artículo científico publicado en 2004

Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex.

artículo científico publicado en 2008

Nucleation of alpha 1-antichymotrypsin polymerization

artículo científico publicado en 2003

Physical characterization of serpin conformations

artículo científico publicado en 2004

Predicting HLA class I alloantigen immunogenicity from the number and physiochemical properties of amino acid polymorphisms

artículo científico publicado en 2009

Protochlorophyllide oxidoreductase: a homology model examined by site-directed mutagenesis

artículo científico publicado en 2001

Purification of membrane proteins free from conventional detergents: SMA, new polymers, new opportunities and new insights.

artículo científico publicado en 2018

Rapid injection linear dichroism for studying the kinetics of biological processes.

artículo científico publicado en 2012

Restriction Enzyme Kinetics Monitored by UV Linear Dichroism†

artículo científico publicado en 2006

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis

artículo científico publicado en 2019

Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition.

artículo científico publicado en 2009

So how do you know you have a macromolecular complex?

artículo científico publicado en 2006

Structural Implications of Platelet Endothelial Cell Adhesion Molecule-1 Polymorphisms

artículo científico publicado en 2008

Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

artículo científico publicado en 2014

Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53

artículo científico publicado en 2005

Structural basis of substrate specificity in malate dehydrogenases: crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, alpha-ketomalonate and tetrahydoNAD

artículo científico publicado en 1999

Structural characterization of recombinant human CD81 produced in Pichia pastoris

artículo científico publicado en 2007

Structures of the dI2dIII1 complex of proton-translocating transhydrogenase with bound, inactive analogues of NADH and NADPH reveal active site geometries

artículo científico publicado en 2007

Substitution of tyrosine 146 in the dI component of proton-translocating transhydrogenase leads to reversible dissociation of the active dimer into inactive monomers

artículo científico publicado en 2007

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a.

artículo científico publicado en 2014

Surfactant-free purification of membrane proteins with intact native membrane environment

artículo científico publicado en 2011

Synchrotron radiation linear dichroism spectroscopy of the antibiotic peptide gramicidin in lipid membranes.

artículo científico publicado en 2009

Targeting a surface cavity of alpha 1-antitrypsin to prevent conformational disease

artículo científico publicado en 2003

Tetramerization of ZapA is required for FtsZ bundling.

artículo científico publicado en 2013

The N‐terminal segment of antithrombin acts as a steric gate for the binding of heparin ⬇️

artículo científico publicado el 1 de marzo de 1998

The desmoglein-specific cytoplasmic region is intrinsically disordered in solution and interacts with multiple desmosomal protein partners

artículo científico publicado en 2008

The influence of poly(styrene-co-maleic acid) copolymer structure on the properties and self-assembly of SMALP nanodiscs

artículo científico publicado en 2017

The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis

artículo científico publicado en 2001

The pH sensitivity of murine heat shock protein 47 (HSP47) binding to collagen is affected by mutations in the breach histidine cluster.

artículo científico publicado en 2012

The synergistic action of melittin and phospholipase A2 with lipid membranes: development of linear dichroism for membrane-insertion kinetics.

artículo científico publicado en 2010

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. ⬇️

artículo científico publicado en 2014

Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure. ⬇️

artículo científico publicado en 2017

Validation of new microvolume Couette flow linear dichroism cells

artículo científico publicado en 2005

α-1-antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease

artículo científico publicado en 2015

Lista de obras de Tim Dafforn

6-mer peptide selectively anneals to a pathogenic serpin conformation and blocks polymerization. Implications for the prevention of Z alpha(1)-antitrypsin-related cirrhosis

A Kinetic Mechanism for the Polymerization of α1-Antitrypsin

A cluster of familial Creutzfeldt-Jakob disease mutations recapitulate conserved residues in Doppel: a case of molecular mimicry?

A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex.

A general method for relieving substrate inhibition in lactate dehydrogenases

A method for detergent-free isolation of membrane proteins in their local lipid environment. ⬇️

A novel model and molecular therapy for Z alpha-1 antitrypsin deficiency.

A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain

An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles.

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

Antithrombins Wibble and Wobble (T85M/K): archetypal conformational diseases with in vivo latent-transition, thrombosis, and heparin activation.

Assembly pathway of a designed alpha-helical protein fiber.

Automated High-Throughput Capillary Circular Dichroism and Intrinsic Fluorescence Spectroscopy for Rapid Determination of Protein Structure

Breaking the 200 nm limit for routine flow linear dichroism measurements using UV synchrotron radiation.

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum.

Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila

Characterizing the assembly of the Sup35 yeast prion fragment, GNNQQNY: structural changes accompany a fiber-to-crystal switch.

Correlation of the enzyme activities of Bacillus stearothermophilus lactate dehydrogenase on three substrates with the results of molecular dynamics/energy minimization conformational searching

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.

De novo design of Ln(III) coiled coils for imaging applications.

Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroism.

Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs

Detergent-free purification of ABC (ATP-binding-cassette) transporters.

Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle

Drosophila necrotic mutations mirror disease-associated variants of human serpins

Dye Aggregate-Mediated Self-Assembly of Bacteriophage Bioconjugates

Encapsulated membrane proteins: A simplified system for molecular simulation.

Expression, purification and crystallization of the cell-division protein YgfE from Escherichia coli.

Flow linear dichroism of some prototypical proteins.

Fluorescence detected linear dichroism spectroscopy: A selective and sensitive probe for fluorophores in flow-oriented systems.

Folding and Membrane Insertion of the Pore-Forming Peptide Gramicidin Occur as a Concerted Process

FtsZ fiber bundling is triggered by a conformational change in bound GTP.

FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP.

Functional and biophysical analysis of the C-terminus of the CGRP-receptor; a family B GPCR.

Functional domains of the human epididymal protease inhibitor, eppin

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential.

Golgi protein FAPP2 tubulates membranes

Heteropolymerization of S, I, and Z alpha1-antitrypsin and liver cirrhosis.

High-resolution, three-dimensional modeling of human leukocyte antigen class I structure and surface electrostatic potential reveals the molecular basis for alloantibody binding epitopes

Identification and characterization of trimethylamine N-oxide (TMAO) demethylase and TMAO permease in Methylocella silvestris BL2.

Identification of a 4-mer Peptide Inhibitor that Effectively Blocks the Polymerization of Pathogenic Z α1-Antitrypsin

Identification of the primary collagen-binding surface on human glycoprotein VI by site-directed mutagenesis and by a blocking phage antibody

Inhibiting polymerization: new therapeutic strategies for Z alpha1-antitrypsin-related emphysema

Inhibitory activity of the Drosophila melanogaster serpin Necrotic is dependent on lysine residues in the D-helix

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

Kinetic study of the thermal denaturation of a hyperthermostable extracellular α-amylase from Pyrococcus furiosus.

Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

Linear dichroism for the detection of single base pair mutationsElectronic supplementary information (ESI) available: Figures S1–S4. See http://www.rsc.org/suppdata/cc/b1/b107830p/

Linear dichroism of biomolecules: which way is up?

Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold

Location of auxilin within a clathrin cage.

Looking at long molecules in solution: what happens when they are subjected to Couette flow?

Membrane protein extraction and purification using styrene-maleic acid (SMA) copolymer: effect of variations in polymer structure.

Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.

Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer

Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism

Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis.

Molecular studies of anti-HLA-A2 using light-chain shuffling: a structural model for HLA antibody binding.

Multifunctional graphene oxide-bacteriophage based porous three-dimensional micro-nanocomposites

Murine serpin 2A is a redox-sensitive intracellular protein.

Mutation of M13 Bacteriophage Major Coat Protein for Increased Conjugation to Exogenous Compounds

Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ

Natively unfolded domains in endocytosis: hooks, lines and linkers.

Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex.

Nucleation of alpha 1-antichymotrypsin polymerization

Physical characterization of serpin conformations

Predicting HLA class I alloantigen immunogenicity from the number and physiochemical properties of amino acid polymorphisms

Protochlorophyllide oxidoreductase: a homology model examined by site-directed mutagenesis

Purification of membrane proteins free from conventional detergents: SMA, new polymers, new opportunities and new insights.

Rapid injection linear dichroism for studying the kinetics of biological processes.

Restriction Enzyme Kinetics Monitored by UV Linear Dichroism†

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis

Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition.

So how do you know you have a macromolecular complex?

Structural Implications of Platelet Endothelial Cell Adhesion Molecule-1 Polymorphisms

Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53

Structural basis of substrate specificity in malate dehydrogenases: crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, alpha-ketomalonate and tetrahydoNAD