Lista de obras - Knud J Jensen - Dominio Público Uruguay

3- Instead of 4-helix formation in a de novo designed protein in solution revealed by small-angle X-ray scattering

artículo científico publicado en 2008

A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases.

artículo científico publicado en 2017

A cyclic peptidic serine protease inhibitor: increasing affinity by increasing peptide flexibility

artículo científico publicado en 2014

A de Novo-Designed Monomeric, Compact Three-Helix-Bundle Protein on a Carbohydrate Template

artículo científico publicado en 2015

A high-throughput O-glycopeptide discovery platform for seromic profiling.

artículo científico publicado en 2010

A new, efficient glycosylation method for oligosaccharide synthesis under neutral conditions: preparation and use of new DISAL donors

artículo científico publicado en 2001

Additional disulfide bonds in insulin: Prediction, recombinant expression, receptor binding affinity, and stability.

artículo científico publicado en 2015

An intermolecular binding mechanism involving multiple LysM domains mediates carbohydrate recognition by an endopeptidase.

artículo científico publicado en 2015

Antimicrobial peptide shows enhanced activity and reduced toxicity upon grafting to chitosan polymers.

artículo científico publicado en 2015

Antisense Oligonucleotides Internally Labeled with Peptides Show Improved Target Recognition and Stability to Enzymatic Degradation

artículo científico publicado en 2016

Automated 'X-Y' robot for peptide synthesis with microwave heating: application to difficult peptide sequences and protein domains

artículo científico publicado en 2010

Backbone amide linker strategy: protocols for the synthesis of C-terminal peptide aldehydes

artículo científico publicado en 2013

COMU: scope and limitations of the latest innovation in peptide acyl transfer reagents.

artículo científico

Carbohydrates in peptide and protein design.

artículo científico publicado en 2005

Carbopeptides: carbohydrates as potential templates for de novo design of protein models

scientific article published on 01 July 2000

Carbopeptides: chemoselective ligation of peptide aldehydes to an aminooxy-functionalized D-galactose template

artículo científico publicado en 2000

Carboproteins: a 4-alpha-helix bundle protein model assembled on a D-galactopyranoside template

artículo científico publicado en 2001

Characterization of the viral O-glycopeptidome: a novel tool of relevance for vaccine design and serodiagnosis

artículo científico publicado en 2012

Chemical Strategies for Half-Life Extension of Biopharmaceuticals: Lipidation and Its Alternatives

artículo científico publicado en 2018

Chemical synthesis and receptor binding of catfish somatostatin: a disulfide-bridged beta-D-Galp-(1-->3)-alpha-D-GalpNAc O-glycopeptide

artículo científico publicado en 2000

Chemically synthesized 58-mer LysM domain binds lipochitin oligosaccharide

artículo científico

Chemoselective Reactions for the Synthesis of Glycoconjugates from Unprotected Carbohydrates

artículo científico publicado en 2017

Chemoselective capture of glycans for analysis on gold nanoparticles: carbohydrate oxime tautomers provide functional recognition by proteins.

artículo científico publicado en 2009

Construction of Insulin 18-mer Nanoassemblies Driven by Coordination to Iron(II) and Zinc(II) Ions at Distinct Sites

artículo científico publicado en 2016

Controlled self-assembly of re-engineered insulin by Fe(II)

artículo científico publicado en 2011

DISAL Glycosyl Donors for the Synthesis of a Linear Hexasaccharide under Mild Conditions

artículo científico publicado en 2003

Dimeric peptides with three different linkers self-assemble with phospholipids to form peptide nanodiscs that stabilize membrane proteins.

artículo científico publicado en 2016

Direct chemoselective synthesis of glyconanoparticles from unprotected reducing glycans and glycopeptide aldehydes

artículo científico publicado en 2009

Effect of Residual Water and Microwave Heating on the Half‐Life of the Reagents and Reactive Intermediates in Peptide Synthesis ⬇️

artículo científico publicado el 13 de junio de 2012

Efficient synthesis of glycosylated phenazine natural products and analogs with DISAL (methyl 3,5-dinitrosalicylate) glycosyl donors

artículo científico publicado en 2003

End-to-end assembly of gold nanorods via oligopeptide linking and surfactant control

artículo científico publicado en 2012

Expression, receptor binding, and biophysical characterization of guinea pig insulin desB30: a monomeric insulin variant.

artículo científico publicado en 2015

Farnesylated peptides in model membranes: a biophysical investigation.

artículo científico publicado en 2003

Fmoc Solid-Phase Synthesis of Peptide Thioesters by Masking as Trithioortho Esters

artículo científico publicado en 2003

Fmoc solid-phase synthesis of C-terminal peptide thioesters by formation of a backbone pyroglutamyl imide moiety

artículo científico publicado en 2009

Folding Topology of a Short Coiled-Coil Peptide Structure Templated by an Oligonucleotide Triplex

artículo científico publicado en 2017

Fractionation, solid-phase immobilization and chemical degradation of long pectin oligogalacturonides. Initial steps towards sequencing of oligosaccharides.

artículo científico publicado en 2005

GUB06-046, a novel secretin/glucagon-like peptide 1 co-agonist, decreases food intake, improves glycemic control, and preserves beta cell mass in diabetic mice.

artículo científico publicado en 2017

General solid-phase phosphopeptide proteomis with affinity pull-down.

artículo científico publicado en 2009

Glyco-scan: varying glycosylation in the sequence of the peptide hormone PYY3-36 and its effect on receptor selectivity

scientific article published on 01 February 2010

Glycoconjugate Oxime Formation Catalyzed at Neutral pH: Mechanistic Insights and Applications of 1,4-Diaminobenzene as Superior Catalyst for Complex Carbohydrates

artículo científico publicado en 2018

Half-Life Extending Modifications of Peptide YY3-36 Direct Receptor-Mediated Internalization

artículo científico publicado en 2019

Half-Life Extension of Biopharmaceuticals using Chemical Methods: Alternatives to PEGylation.

artículo científico publicado en 2016

Hierarchical Self-Assembly of Designed 2 × 2-α-Helix Bundle Proteins on Au(111) Surfaces

artículo científico publicado en 2006

Identification of anchor points for chemical modification of a small cysteine-rich protein by using a cysteine scan.

artículo científico publicado en 2011

Immobilization of pectin fragments on solid supports: novel coupling by thiazolidine formation

artículo científico publicado en 2002

Impact of chain length on antibacterial activity and hemocompatibility of quaternary N-alkyl and n,n-dialkyl chitosan derivatives.

artículo científico publicado en 2015

Improved PET imaging of uPAR expression using new (64)Cu-labeled cross-bridged peptide ligands: comparative in vitro and in vivo studies

artículo científico publicado en 2013

Improved characterization of nod factors and genetically based variation in LysM Receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.

artículo científico publicado en 2010

Improving membrane binding as a design strategy for amphipathic peptide hormones: 2-helix variants of PYY3-36

artículo científico publicado en 2012

Instruments for automated peptide synthesis.

artículo científico publicado en 2013

Integrin Targeting and Toxicological Assessment of Peptide-Conjugated Liposome Delivery Systems to Activated Endothelial Cells

artículo científico publicado en 2016

Interconversion of active and inactive conformations of urokinase-type plasminogen activator

artículo científico publicado en 2012

Intramolecular glycosylation under neutral conditions for synthesis of 1,4-linked disaccharides

artículo científico publicado en 2001

Kinetic analysis of inhibition of glucoamylase and active site mutants via chemoselective oxime immobilization of acarbose on SPR chip surfaces

artículo científico

Large-Scale Biophysical Evaluation of Protein PEGylation Effects: In Vitro Properties of 61 Protein Entities

artículo científico publicado en 2016

Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding.

artículo científico publicado en 2012

Leonidas Zervas award lecture: abiotic ligands for new quaternary architectures of peptides and proteins.

artículo científico

Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

artículo científico publicado en 2020

Linear Multiepitope (Glyco)peptides for Type-Specific Serology of Herpes Simplex Virus (HSV) Infections.

artículo científico publicado en 2017

Linkers, resins, and general procedures for solid-phase peptide synthesis

artículo científico publicado en 2013

Lipochitin oligosaccharides immobilized through oximes in glycan microarrays bind LysM proteins

artículo científico

Membrane Curvature and Lipid Composition Synergize To Regulate N-Ras Anchor Recruitment

artículo científico publicado en 2017

Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases

artículo científico publicado en 2015

Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells.

artículo científico publicado en 2017

Metal Ion Controlled Self-Assembly of a Chemically Reengineered Protein Drug Studied by Small-Angle X-ray Scattering

scientific article published on 13 August 2012

Microwave heating in solid-phase peptide synthesis ⬇️

artículo científico publicado el 20 de octubre de 2011

Microwave-assisted solid-phase peptide synthesis using the biotage Syro Wave™.

artículo científico publicado en 2013

Modifying the conserved C-terminal tyrosine of the peptide hormone PYY3-36 to improve Y2 receptor selectivity

artículo científico publicado en 2009

Monolayer assemblies of a de novo designed 4-alpha-helix bundle carboprotein and its sulfur anchor fragment on Au(111) surfaces addressed by voltammetry and in situ scanning tunneling microscopy

artículo científico publicado en 2003

Monolayers of a de novo designed 4-alpha-helix bundle carboprotein and partial structures on Au(111)-surfaces.

artículo científico publicado en 2002

Monosaccharide templates for de novo designed 4-alpha-helix bundle proteins: template effects in carboproteins.

artículo científico publicado en 2003

Neoglycolipids for Prolonging the Effects of Peptides: Self-Assembling Glucagon-like Peptide 1 Analogues with Albumin Binding Properties and Potent in Vivo Efficacy.

artículo científico publicado en 2016

New dendrimer-peptide host-guest complexes: towards dendrimers as peptide carriers.

artículo científico publicado en 2002

Novel Covalently Linked Insulin Dimer Engineered to Investigate the Function of Insulin Dimerization

artículo científico publicado en 2012

Nucleophilic catalysis of carbohydrate oxime formation by anilines.

artículo científico publicado en 2010

Peptide Half-Life Extension: Divalent, Small-Molecule Albumin Interactions Direct the Systemic Properties of Glucagon-Like Peptide 1 (GLP-1) Analogues.

artículo científico publicado en 2017

Peptide hormone isoforms: N-terminally branched PYY3-36 isoforms give improved lipid and fat-cell metabolism in diet-induced obese mice

scientific article published on 01 November 2010

Peptide release, side-chain deprotection, work-up, and isolation.

artículo científico publicado en 2013

Peptide-Stabilized, Fluorescent Silver Nanoclusters: Solid-Phase Synthesis and Screening

artículo científico publicado en 2016

Peptide-oligonucleotide conjugates as nanoscale building blocks for assembly of an artificial three-helix protein mimic.

artículo científico publicado en 2016

Perfluoroalkyl Chains Direct Novel Self-Assembly of Insulin

scientific article published on 22 December 2011

Polyamine-functionalized 2'-amino-LNA in oligonucleotides: Facile synthesis of novel monomers and high affinity binding towards ssDNA and dsDNA

artículo científico publicado en 2020

Predictive mutagenesis of ligation-independent cloning (LIC) vectors for protein expression and site-specific chemical conjugation.

artículo científico publicado en 2011

Preparation of glycoconjugates from unprotected carbohydrates for protein-binding studies

artículo científico publicado en 2017

Probing protein phosphatase substrate binding: affinity pull-down of ILKAP phosphatase 2C with phosphopeptides

artículo científico publicado en 2012

Random Glycopeptide Bead Libraries for Seromic Biomarker Discovery ⬇️

artículo científico publicado el 2 de noviembre de 2010

Receptor-mediated exopolysaccharide perception controls bacterial infection.

artículo científico publicado en 2015

Reconsidering glycosylations at high temperature: precise microwave heating

artículo científico publicado en 2005

Reconstructing the Origins of the Somatostatin and Allatostatin-C Signaling Systems Using the Accelerated Evolution of Biodiverse Cone Snail Toxins

artículo científico publicado en 2022

Regioselective fluorescent labeling of N,N,N-trimethyl chitosan via oxime formation.

artículo científico publicado en 2012

Role of the peri-effect in synthesis and reactivity of highly substituted naphthaldehydes: a novel backbone amide linker for solid-phase synthesis.

artículo científico publicado en 2004

Selection of High-Affinity Peptidic Serine Protease Inhibitors with Increased Binding Entropy from a Back-Flip Library of Peptide-Protease Fusions

artículo científico publicado en 2015

Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

scientific article published in Nature Communications

Self-assembling peptides form nanodiscs that stabilize membrane proteins.

artículo científico publicado en 2014

Self-assembly of DNA-peptide supermolecules: Coiled-coil peptide structures templated by D-DNA and L-DNA triplexes exhibit chirality-independent but orientation-dependent stabilizing cooperativity

artículo científico publicado en 2020

Self-assembly of designed coiled coil peptides studied by small-angle X-ray scattering and analytical ultracentrifugation

artículo científico publicado en 2013

Site-selective three-component reaction for dual-functionalization of peptides

artículo científico publicado en 2013

Small-molecule affinity ligands for protein purification: combined computational enrichment and automated in-line screening of an optically encoded library

artículo científico publicado en 2010

Softening of POPC membranes by magainin.

artículo científico publicado en 2008

Solid-Phase Synthesis with Tris(alkoxy)benzyl Backbone Amide Linkage (BAL)[≠]

Solid-phase chemical tools for glycobiology.

artículo científico publicado en 2006

Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases

artículo científico publicado en 2002

Solid-phase peptide synthesis: an introduction

artículo científico publicado en 2013

Solid-phase synthesis of new saphenamycin analogues with antimicrobial activity

artículo científico publicado en 2002

Solid-phase synthesis of phosphopeptides

artículo científico publicado en 2013

Solid-supported enzymatic synthesis of pectic oligogalacturonides and their analysis by MALDI-TOF mass spectrometry.

artículo científico publicado en 2003

Synchrotron radiation circular dichroism spectroscopy applied to metmyoglobin and a 4-alpha-helix bundle carboprotein

artículo científico publicado en 2005

Synergy of Two Highly Specific Biomolecular Recognition Events: Aligning an AT-Hook Peptide in DNA Minor Grooves via Covalent Conjugation to 2'-Amino-LNA.

artículo científico publicado en 2018

Synthesis and evaluation of novel lipidated neuromedin U analogs with increased stability and effects on food intake

artículo científico publicado en 2014

Synthesis of C-terminal peptide thioesters using Fmoc-based solid-phase peptide chemistry

artículo científico publicado en 2013

Synthesis of N-methylated peptides: on-resin methylation and microwave-assisted couplings

artículo científico publicado en 2013

Synthesis of benzaldehyde-functionalized glycans: a novel approach towards glyco-SAMs as a tool for surface plasmon resonance studies.

artículo científico publicado en 2010

Synthesis of functionalized de novo designed 8–16 kDa model proteins towards metal ion-binding and esterase activity

article

THAL, a sterically unhindered linker for the solid-phase synthesis of acid-sensitive protected peptide acids

artículo científico publicado en 2008

The binding mechanism of a peptidic cyclic serine protease inhibitor

artículo científico publicado en 2011

The ortho backbone amide linker (o-BAL) is an easily prepared and highly acid-labile handle for solid-phase synthesis

artículo científico publicado en 2002

The road to the first, fully active and more stable human insulin variant with an additional disulfide bond.

artículo científico

Thiophene backbone amide linkers, a new class of easily prepared and highly acid-labile linkers for solid-phase synthesis

artículo científico publicado en 2006

Total synthesis of desB30 insulin analogues by biomimetic folding of single-chain precursors

artículo científico publicado en 2008

Two dialkoxynaphthalene aldehydes as backbone amide linkers for solid-phase synthesis.

artículo científico publicado en 2004

Ultramild protein-mediated click chemistry creates efficient oligonucleotide probes for targeting and detecting nucleic acids

artículo científico

Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs

artículo científico publicado en 2019

Lista de obras de Knud J Jensen

3- Instead of 4-helix formation in a de novo designed protein in solution revealed by small-angle X-ray scattering

A brain-targeting lipidated peptide for neutralizing RNA-mediated toxicity in Polyglutamine Diseases.

A cyclic peptidic serine protease inhibitor: increasing affinity by increasing peptide flexibility

A de Novo-Designed Monomeric, Compact Three-Helix-Bundle Protein on a Carbohydrate Template

A high-throughput O-glycopeptide discovery platform for seromic profiling.

A new, efficient glycosylation method for oligosaccharide synthesis under neutral conditions: preparation and use of new DISAL donors

Additional disulfide bonds in insulin: Prediction, recombinant expression, receptor binding affinity, and stability.

An intermolecular binding mechanism involving multiple LysM domains mediates carbohydrate recognition by an endopeptidase.

Antimicrobial peptide shows enhanced activity and reduced toxicity upon grafting to chitosan polymers.

Antisense Oligonucleotides Internally Labeled with Peptides Show Improved Target Recognition and Stability to Enzymatic Degradation

Automated 'X-Y' robot for peptide synthesis with microwave heating: application to difficult peptide sequences and protein domains

Backbone amide linker strategy: protocols for the synthesis of C-terminal peptide aldehydes

COMU: scope and limitations of the latest innovation in peptide acyl transfer reagents.

Carbohydrates in peptide and protein design.

Carbopeptides: carbohydrates as potential templates for de novo design of protein models

Carbopeptides: chemoselective ligation of peptide aldehydes to an aminooxy-functionalized D-galactose template

Carboproteins: a 4-alpha-helix bundle protein model assembled on a D-galactopyranoside template

Characterization of the viral O-glycopeptidome: a novel tool of relevance for vaccine design and serodiagnosis

Chemical Strategies for Half-Life Extension of Biopharmaceuticals: Lipidation and Its Alternatives

Chemical synthesis and receptor binding of catfish somatostatin: a disulfide-bridged beta-D-Galp-(1-->3)-alpha-D-GalpNAc O-glycopeptide

Chemically synthesized 58-mer LysM domain binds lipochitin oligosaccharide

Chemoselective Reactions for the Synthesis of Glycoconjugates from Unprotected Carbohydrates

Chemoselective capture of glycans for analysis on gold nanoparticles: carbohydrate oxime tautomers provide functional recognition by proteins.

Construction of Insulin 18-mer Nanoassemblies Driven by Coordination to Iron(II) and Zinc(II) Ions at Distinct Sites

Controlled self-assembly of re-engineered insulin by Fe(II)

DISAL Glycosyl Donors for the Synthesis of a Linear Hexasaccharide under Mild Conditions

Dimeric peptides with three different linkers self-assemble with phospholipids to form peptide nanodiscs that stabilize membrane proteins.

Direct chemoselective synthesis of glyconanoparticles from unprotected reducing glycans and glycopeptide aldehydes

Effect of Residual Water and Microwave Heating on the Half‐Life of the Reagents and Reactive Intermediates in Peptide Synthesis ⬇️

Efficient synthesis of glycosylated phenazine natural products and analogs with DISAL (methyl 3,5-dinitrosalicylate) glycosyl donors

End-to-end assembly of gold nanorods via oligopeptide linking and surfactant control

Expression, receptor binding, and biophysical characterization of guinea pig insulin desB30: a monomeric insulin variant.

Farnesylated peptides in model membranes: a biophysical investigation.

Fmoc Solid-Phase Synthesis of Peptide Thioesters by Masking as Trithioortho Esters

Fmoc solid-phase synthesis of C-terminal peptide thioesters by formation of a backbone pyroglutamyl imide moiety

Folding Topology of a Short Coiled-Coil Peptide Structure Templated by an Oligonucleotide Triplex

Fractionation, solid-phase immobilization and chemical degradation of long pectin oligogalacturonides. Initial steps towards sequencing of oligosaccharides.

GUB06-046, a novel secretin/glucagon-like peptide 1 co-agonist, decreases food intake, improves glycemic control, and preserves beta cell mass in diabetic mice.

General solid-phase phosphopeptide proteomis with affinity pull-down.

Glyco-scan: varying glycosylation in the sequence of the peptide hormone PYY3-36 and its effect on receptor selectivity

Glycoconjugate Oxime Formation Catalyzed at Neutral pH: Mechanistic Insights and Applications of 1,4-Diaminobenzene as Superior Catalyst for Complex Carbohydrates

Half-Life Extending Modifications of Peptide YY3-36 Direct Receptor-Mediated Internalization

Half-Life Extension of Biopharmaceuticals using Chemical Methods: Alternatives to PEGylation.

Hierarchical Self-Assembly of Designed 2 × 2-α-Helix Bundle Proteins on Au(111) Surfaces

Identification of anchor points for chemical modification of a small cysteine-rich protein by using a cysteine scan.

Immobilization of pectin fragments on solid supports: novel coupling by thiazolidine formation

Impact of chain length on antibacterial activity and hemocompatibility of quaternary N-alkyl and n,n-dialkyl chitosan derivatives.

Improved PET imaging of uPAR expression using new (64)Cu-labeled cross-bridged peptide ligands: comparative in vitro and in vivo studies

Improved characterization of nod factors and genetically based variation in LysM Receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.

Improving membrane binding as a design strategy for amphipathic peptide hormones: 2-helix variants of PYY3-36

Instruments for automated peptide synthesis.

Integrin Targeting and Toxicological Assessment of Peptide-Conjugated Liposome Delivery Systems to Activated Endothelial Cells

Interconversion of active and inactive conformations of urokinase-type plasminogen activator

Intramolecular glycosylation under neutral conditions for synthesis of 1,4-linked disaccharides

Kinetic analysis of inhibition of glucoamylase and active site mutants via chemoselective oxime immobilization of acarbose on SPR chip surfaces

Large-Scale Biophysical Evaluation of Protein PEGylation Effects: In Vitro Properties of 61 Protein Entities

Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding.

Leonidas Zervas award lecture: abiotic ligands for new quaternary architectures of peptides and proteins.

Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Linear Multiepitope (Glyco)peptides for Type-Specific Serology of Herpes Simplex Virus (HSV) Infections.

Linkers, resins, and general procedures for solid-phase peptide synthesis

Lipochitin oligosaccharides immobilized through oximes in glycan microarrays bind LysM proteins

Membrane Curvature and Lipid Composition Synergize To Regulate N-Ras Anchor Recruitment

Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases

Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells.

Metal Ion Controlled Self-Assembly of a Chemically Reengineered Protein Drug Studied by Small-Angle X-ray Scattering

Microwave heating in solid-phase peptide synthesis ⬇️

Microwave-assisted solid-phase peptide synthesis using the biotage Syro Wave™.

Modifying the conserved C-terminal tyrosine of the peptide hormone PYY3-36 to improve Y2 receptor selectivity

Monolayer assemblies of a de novo designed 4-alpha-helix bundle carboprotein and its sulfur anchor fragment on Au(111) surfaces addressed by voltammetry and in situ scanning tunneling microscopy

Monolayers of a de novo designed 4-alpha-helix bundle carboprotein and partial structures on Au(111)-surfaces.

Monosaccharide templates for de novo designed 4-alpha-helix bundle proteins: template effects in carboproteins.

Neoglycolipids for Prolonging the Effects of Peptides: Self-Assembling Glucagon-like Peptide 1 Analogues with Albumin Binding Properties and Potent in Vivo Efficacy.

New dendrimer-peptide host-guest complexes: towards dendrimers as peptide carriers.

Novel Covalently Linked Insulin Dimer Engineered to Investigate the Function of Insulin Dimerization

Nucleophilic catalysis of carbohydrate oxime formation by anilines.

Peptide Half-Life Extension: Divalent, Small-Molecule Albumin Interactions Direct the Systemic Properties of Glucagon-Like Peptide 1 (GLP-1) Analogues.

Peptide hormone isoforms: N-terminally branched PYY3-36 isoforms give improved lipid and fat-cell metabolism in diet-induced obese mice

Peptide release, side-chain deprotection, work-up, and isolation.