Lista de obras - Martin R Webb - Dominio Público Uruguay

17 The stereochemical course of nucleoside triphosphatase reactions

artículo científico publicado el 1 de enero de 1982

A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase.

artículo científico publicado en 2015

A Secondary beta Deuterium Kinetic Isotope Effect in the Chorismate Synthase Reaction

artículo científico publicado en 2000

A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases

artículo científico publicado en 2011

A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein

scientific article published on 01 December 2006

A continuous enzyme-coupled assay for triphosphohydrolase activity of HIV-1 restriction factor SAMHD1

artículo científico publicado en 2014

A fluorescent sensor of the phosphorylation state of nucleoside diphosphate kinase and its use to monitor nucleoside diphosphate concentrations in real time

artículo científico publicado en 2001

A fluorescent, reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM.

artículo científico publicado en 2010

A kinetic analysis of the nucleotide-induced allosteric transitions of GroEL.

artículo científico publicado en 1999

A method for determining the positional isotope exchange in a nucleoside triphosphate: cyclization of nucleoside triphosphate by dicyclohexylcarbodiimide.

artículo científico publicado en 1980

A new method for the time-resolved measurement of phosphate release in permeabilized muscle fibers

artículo científico publicado en 1995

A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin

artículo científico publicado en 2004

A tale of the unexpected

artículo científico publicado en 2006

ATPase and shortening rates in frog fast skeletal myofibrils by time-resolved measurements of protein-bound and free Pi.

artículo científico publicado en 1998

ATPase cycle and DNA unwinding kinetics of RecG helicase

artículo científico publicado en 2012

Absence of a phosphorylated intermediate during ATP hydrolysis by Escherichia coli transcription termination protein rho.

artículo científico publicado en 1986

Activation of NADPH oxidase involves the dissociation of p21rac from its inhibitory GDP/GTP exchange protein (rhoGDI) followed by its translocation to the plasma membrane.

artículo científico publicado en 1994

Analysis of chiral inorganic [16O, 17O, 18O]thiophosphate and the stereochemistry of the 3-phosphoglycerate kinase reaction

artículo científico publicado en 1980

Analysis of the ATPase mechanism of myosin subfragment 1 from insect fibrillar flight muscle in the presence and absence of actin, using phosphate-water oxygen exchange measurements

artículo científico publicado en 1987

Bipolar DNA translocation contributes to highly processive DNA unwinding by RecBCD enzyme

artículo científico publicado en 2005

Changes in the ATPase activity of insect fibrillar flight muscle during calcium and strain activation probed by phosphate-water oxygen exchange

artículo científico publicado en 1987

Changes in the ATPase activity of insect fibrillar flight muscle during sinusoidal length oscillation probed by phosphate-water oxygen exchange

artículo científico publicado en 1988

Characterization of the GTPase reaction of elongation factor Tu. Determination of the stereochemical course in the presence of antibiotic X5108 ⬇️

artículo científico publicado el 10 de mayo de 1982

Cross-bridge kinetics in asynchronous insect flight muscle

artículo científico publicado en 1988

Crossbridge cycle in smooth muscle: kinetics assessed with flash photolysis and fluorescent probes.

artículo científico publicado en 2004

Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate

artículo científico publicado en 1998

Deconvoluting AMP-activated protein kinase (AMPK) adenine nucleotide binding and sensing

artículo científico

Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: measurement of step size and translocation speed

artículo científico publicado en 2000

Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity

artículo científico publicado en 2011

Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

artículo científico publicado en 2015

Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase

artículo científico publicado en 2017

Direct measurement of single-stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine

artículo científico publicado en 2002

EPR studies of the Mn(II) complex with elongation factor Tu and GDP Identification of oxygen ligands to Mn(II) by observation of 17O superhyperfine coupling

scientific article published on 01 November 1981

Electron paramagnetic resonance studies of MN(II) complexes with myosin subfragment 1 and oxygen 17-labeled ligands

artículo científico publicado en 1982

Evidence for a novel, strongly bound acto-S1 complex carrying ADP and phosphate stabilized in the G680V mutant of Dictyostelium myosin II.

artículo científico publicado en 2002

Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate ⬇️

artículo científico publicado el 28 de febrero de 1984

Evidence that phosphate release is the rate-limiting step on the overall ATPase of psoas myofibrils prevented from shortening by chemical cross-linking.

artículo científico publicado en 2002

Fluorescence detection of GDP in real time with the reagentless biosensor rhodamine-ParM.

artículo científico publicado en 2011

Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force

artículo científico publicado en 2010

Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres.

artículo científico publicado en 2007

Fluorescence tools to measure helicase activity in real time.

artículo científico publicado en 2010

Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology ⬇️

artículo científico publicado el 1 de enero de 2011

Fluorescent SSB as a reagentless biosensor for single-stranded DNA.

artículo científico publicado en 2012

Fluorescent biosensors to investigate helicase activity

artículo científico publicado en 2010

Fluorescent biosensors: design and application to motor proteins

artículo científico

Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin

artículo científico publicado en 2001

Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity

artículo científico publicado en 2008

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA.

artículo científico publicado en 2018

Helicase unwinding: active or merely perfect?

artículo científico publicado en 2012

Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site

artículo científico publicado en 1990

Interaction of GTPase activating proteins (GAPs) with p21ras measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21ras.

artículo científico publicado en 1993

Kinetic analysis of the hydrolysis of GTP by p21N-ras. The basal GTPase mechanism.

artículo científico publicado en 1988

Kinetic mechanism of initiation by RepD as a part of asymmetric, rolling circle plasmid unwinding.

artículo científico publicado en 2012

Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue

artículo científico publicado en 2006

Kinetics of ADP dissociation from the trail and lead heads of actomyosin V following the power stroke

artículo científico publicado en 2007

Kinetics of ATP release and Pi binding during the ATPase cycle of lethocerus flight muscle fibres, using phosphate-water oxygen exchange

artículo científico publicado en 1991

Kinetics of Nucleoside Triphosphate Cleavage and Phosphate Release Steps by Associated Rabbit Skeletal Actomyosin, Measured Using a Novel Fluorescent Probe for Phosphate †

artículo científico publicado en 1997

Kinetics of inorganic phosphate release during the interaction of p21ras with the GTPase-activating proteins, p120-GAP and neurofibromin.

artículo científico publicado en 1995

Kinetics of oxygen-18 exchange between inorganic phosphate and water catalyzed by myosin subfragment 1, using the 18O shift in 31P NMR ⬇️

artículo científico publicado el 10 de mayo de 1978

Ligand-dependent dynamics of the active-site lid in bacterial dimethylarginine dimethylaminohydrolase.

scientific article published on 07 February 2014

Measurement of the reversibility of ATP binding to myosin in calcium-activated skinned fibers from rabbit skeletal muscle. Oxygen exchange between water and ATP released to the solution

scientific article published on 01 May 1989

Mechanism of GTP hydrolysis by p21N-ras catalyzed by GAP: studies with a fluorescent GTP analogue

artículo científico publicado en 1993

Mechanism of adenosine 5'-triphosphate cleavage by myosin: studies with oxygen-18-labeled adenosine 5'-triphosphate

artículo científico publicado en 1980

Mechanism of inorganic phosphate interaction with phosphate binding protein from Escherichia coli.

artículo científico publicado en 1998

Mechanism of interaction between single-stranded DNA binding protein and DNA

artículo científico publicado en 2010

Mechanism of translocation and kinetics of DNA unwinding by the helicase RecG.

artículo científico publicado en 2005

Mechanistic basis of 5'-3' translocation in SF1B helicases

artículo científico publicado en 2009

Millisecond-scale biochemical response to change in strain

artículo científico publicado en 2011

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD.

artículo científico publicado en 2013

Mycobacterial ABC transport system: structure of the primary phosphate receptor

artículo científico publicado en 2003

Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.

artículo científico publicado en 2004

Oxygen exchange between Pi in the medium and water during ATP hydrolysis mediated by skinned fibers from rabbit skeletal muscle. Evidence for Pi binding to a force-generating state.

artículo científico publicado en 1986

Oxygen exchange between phosphate and water accompanies calcium-regulated ATPase activity of skinned fibers from rabbit skeletal muscle

scientific article published on 01 March 1985

Pathway of processive ATP hydrolysis by kinesin.

artículo científico publicado en 1995

Phosphate release during microtubule assembly: what stabilizes growing microtubules?

artículo científico publicado en 1999

Phosphorus-31 nuclear magnetic resonance of dihydroxyacetone phosphate in the presence of triosephosphate isomerase. The question of nonproductive binding of the substrate hydrate

artículo científico publicado en 1977

Pre-Steady-State Kinetics of the Microtubule • Kinesin ATPase

Probing nucleotide dissociation from myosin in vitro using microgram quantities of myosin.

artículo científico publicado en 2003

Purine nucleoside phosphorylase: its use in a spectroscopic assay for inorganic phosphate and for removing inorganic phosphate with the aid of phosphodeoxyribomutase

artículo científico publicado en 1998

Raman difference studies of GDP and GTP binding to c-Harvey ras.

artículo científico publicado en 1998

Real-time visual detection of Pi released by flagellar dynein ATPase

artículo científico publicado en 1999

Role of magnesium in nucleotide exchange on the small G protein rac investigated using novel fluorescent Guanine nucleotide analogues.

artículo científico publicado en 2002

Smooth muscle myosin: regulation and properties.

artículo científico publicado en 2004

Structural prediction of a rhodamine-based biosensor and comparison with biophysical data.

artículo científico publicado en 2012

Switch 1 mutation S217A converts myosin V into a low duty ratio motor

artículo científico publicado en 2008

The ATPase cycle of PcrA helicase and its coupling to translocation on DNA.

artículo científico publicado en 2009

The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain

artículo científico publicado en 2010

The crystal structure of human rac1, a member of the rho-family complexed with a GTP analogue

artículo científico publicado en 1997

The efficiency of contraction in rabbit skeletal muscle fibres, determined from the rate of release of inorganic phosphate

artículo científico publicado en 1999

The existence of an electrophilic component in the reaction catalysed by triose phosphate isomerase.

artículo científico publicado en 1974

The hydrolysis of ATP that accompanies actin polymerization is essentially irreversible

scientific article published on 01 August 1988

The interaction between rac1 and its guanine nucleotide dissociation inhibitor (GDI), monitored by a single fluorescent coumarin attached to GDI

artículo científico publicado en 1999

The mechanism of ATP hydrolysis catalyzed by myosin and actomyosin, using rapid reaction techniques to study oxygen exchange

scientific article published on 01 November 1981

The mechanism of Ras GTPase activation by neurofibromin.

artículo científico publicado en 2003

The mechanism of guanosine nucleotide hydrolysis by p21 c-Ha-ras. The stereochemical course of the GTPase reaction

scientific article published on 01 April 1989

The mechanism of smooth muscle caldesmon-tropomyosin inhibition of the elementary steps of the actomyosin ATPase

artículo científico publicado en 2006

The orientation and accessibility of substrates on the active site of triosephosphate isomerase

scientific article published on 01 October 1975

The role of nucleoside triphosphate hydrolysis in transducing systems: p21ras and muscle

artículo científico publicado el 29 de abril de 1992

The stereochemical course of phospho transfer catalyzed by adenylosuccinate synthetase. A reaction pathway via a phosphorylated intermediate with net inversion

scientific article published on 01 March 1984

The stereochemical course of phosphoric residue transfer catalyzed by beef heart mitochondrial ATPase.

artículo científico publicado en 1980

The stereochemical course of phosphoric residue transfer catalyzed by sarcoplasmic reticulum ATPase ⬇️

artículo científico publicado el 25 de mayo de 1981

The stereochemical course of phosphoric residue transfer during the myosin ATPase reaction.

artículo científico publicado en 1980

The stereochemical course of the ribosome-dependent GTPase reaction of elongation factor G from Escherichia coli ⬇️

artículo científico publicado el 10 de agosto de 1981

Time resolved measurements show that phosphate release is the rate limiting step on myofibrillar ATPases.

artículo científico publicado en 1995

Time-resolved measurements of phosphate release by cycling cross-bridges in portal vein smooth muscle

artículo científico publicado en 1998

Transient kinetics of aminoglycoside phosphotransferase(3′)‐IIIa reveals a potential drug target in the antibiotic resistance mechanism ⬇️

artículo científico publicado el 26 de octubre de 2012

Uncoupling DNA translocation and helicase activity in PcrA: direct evidence for an active mechanism.

artículo científico publicado en 2000

Vibrational study of phosphate modes in GDP and GTP and their interaction with magnesium in aqueous solution

artículo científico publicado en 1998

Visualizing helicases unwinding DNA at the single molecule level

artículo científico publicado en 2010

Lista de obras de Martin R Webb

17 The stereochemical course of nucleoside triphosphatase reactions

A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase.

A Secondary beta Deuterium Kinetic Isotope Effect in the Chorismate Synthase Reaction

A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases

A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein

A continuous enzyme-coupled assay for triphosphohydrolase activity of HIV-1 restriction factor SAMHD1

A fluorescent sensor of the phosphorylation state of nucleoside diphosphate kinase and its use to monitor nucleoside diphosphate concentrations in real time

A fluorescent, reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM.

A kinetic analysis of the nucleotide-induced allosteric transitions of GroEL.

A method for determining the positional isotope exchange in a nucleoside triphosphate: cyclization of nucleoside triphosphate by dicyclohexylcarbodiimide.

A new method for the time-resolved measurement of phosphate release in permeabilized muscle fibers

A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin

A tale of the unexpected

ATPase and shortening rates in frog fast skeletal myofibrils by time-resolved measurements of protein-bound and free Pi.

ATPase cycle and DNA unwinding kinetics of RecG helicase

Absence of a phosphorylated intermediate during ATP hydrolysis by Escherichia coli transcription termination protein rho.

Activation of NADPH oxidase involves the dissociation of p21rac from its inhibitory GDP/GTP exchange protein (rhoGDI) followed by its translocation to the plasma membrane.

Analysis of chiral inorganic [16O, 17O, 18O]thiophosphate and the stereochemistry of the 3-phosphoglycerate kinase reaction

Analysis of the ATPase mechanism of myosin subfragment 1 from insect fibrillar flight muscle in the presence and absence of actin, using phosphate-water oxygen exchange measurements

Bipolar DNA translocation contributes to highly processive DNA unwinding by RecBCD enzyme

Changes in the ATPase activity of insect fibrillar flight muscle during calcium and strain activation probed by phosphate-water oxygen exchange

Changes in the ATPase activity of insect fibrillar flight muscle during sinusoidal length oscillation probed by phosphate-water oxygen exchange

Characterization of the GTPase reaction of elongation factor Tu. Determination of the stereochemical course in the presence of antibiotic X5108 ⬇️

Cross-bridge kinetics in asynchronous insect flight muscle

Crossbridge cycle in smooth muscle: kinetics assessed with flash photolysis and fluorescent probes.

Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate

Deconvoluting AMP-activated protein kinase (AMPK) adenine nucleotide binding and sensing

Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: measurement of step size and translocation speed

Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity

Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase

Direct measurement of single-stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine

EPR studies of the Mn(II) complex with elongation factor Tu and GDP Identification of oxygen ligands to Mn(II) by observation of 17O superhyperfine coupling

Electron paramagnetic resonance studies of MN(II) complexes with myosin subfragment 1 and oxygen 17-labeled ligands

Evidence for a novel, strongly bound acto-S1 complex carrying ADP and phosphate stabilized in the G680V mutant of Dictyostelium myosin II.

Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate ⬇️

Evidence that phosphate release is the rate-limiting step on the overall ATPase of psoas myofibrils prevented from shortening by chemical cross-linking.

Fluorescence detection of GDP in real time with the reagentless biosensor rhodamine-ParM.

Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force

Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres.

Fluorescence tools to measure helicase activity in real time.

Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology ⬇️

Fluorescent SSB as a reagentless biosensor for single-stranded DNA.

Fluorescent biosensors to investigate helicase activity

Fluorescent biosensors: design and application to motor proteins

Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin

Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA.

Helicase unwinding: active or merely perfect?

Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site

Interaction of GTPase activating proteins (GAPs) with p21ras measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21ras.

Kinetic analysis of the hydrolysis of GTP by p21N-ras. The basal GTPase mechanism.

Kinetic mechanism of initiation by RepD as a part of asymmetric, rolling circle plasmid unwinding.

Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue

Kinetics of ADP dissociation from the trail and lead heads of actomyosin V following the power stroke

Kinetics of ATP release and Pi binding during the ATPase cycle of lethocerus flight muscle fibres, using phosphate-water oxygen exchange

Kinetics of Nucleoside Triphosphate Cleavage and Phosphate Release Steps by Associated Rabbit Skeletal Actomyosin, Measured Using a Novel Fluorescent Probe for Phosphate †

Kinetics of inorganic phosphate release during the interaction of p21ras with the GTPase-activating proteins, p120-GAP and neurofibromin.

Kinetics of oxygen-18 exchange between inorganic phosphate and water catalyzed by myosin subfragment 1, using the 18O shift in 31P NMR ⬇️

Ligand-dependent dynamics of the active-site lid in bacterial dimethylarginine dimethylaminohydrolase.

Measurement of the reversibility of ATP binding to myosin in calcium-activated skinned fibers from rabbit skeletal muscle. Oxygen exchange between water and ATP released to the solution

Mechanism of GTP hydrolysis by p21N-ras catalyzed by GAP: studies with a fluorescent GTP analogue

Mechanism of adenosine 5'-triphosphate cleavage by myosin: studies with oxygen-18-labeled adenosine 5'-triphosphate

Mechanism of inorganic phosphate interaction with phosphate binding protein from Escherichia coli.

Mechanism of interaction between single-stranded DNA binding protein and DNA

Mechanism of translocation and kinetics of DNA unwinding by the helicase RecG.

Mechanistic basis of 5'-3' translocation in SF1B helicases

Millisecond-scale biochemical response to change in strain

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD.

Mycobacterial ABC transport system: structure of the primary phosphate receptor

Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.

Oxygen exchange between Pi in the medium and water during ATP hydrolysis mediated by skinned fibers from rabbit skeletal muscle. Evidence for Pi binding to a force-generating state.

Oxygen exchange between phosphate and water accompanies calcium-regulated ATPase activity of skinned fibers from rabbit skeletal muscle

Pathway of processive ATP hydrolysis by kinesin.

Phosphate release during microtubule assembly: what stabilizes growing microtubules?

Phosphorus-31 nuclear magnetic resonance of dihydroxyacetone phosphate in the presence of triosephosphate isomerase. The question of nonproductive binding of the substrate hydrate

Pre-Steady-State Kinetics of the Microtubule • Kinesin ATPase

Probing nucleotide dissociation from myosin in vitro using microgram quantities of myosin.

Purine nucleoside phosphorylase: its use in a spectroscopic assay for inorganic phosphate and for removing inorganic phosphate with the aid of phosphodeoxyribomutase