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Lista de obras de Shigeaki Harayama

4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer ⬇️

artículo científico publicado el 5 de septiembre de 1992

A Novel Nuclear Export Inhibitor JBIR-02, a New Piericidin Discovered from Streptomyces sp. ML55 ⬇️

artículo científico publicado en 2007

A high-throughput and generic assay method for the determination of substrate specificities of thermophilic alpha-aminotransferases

artículo científico publicado en 2007

A novel phenanthrene dioxygenase from Nocardioides sp. Strain KP7: expression in Escherichia coli.

artículo científico publicado en 2000

A simple and rapid GC/MS method for the simultaneous determination of gaseous metabolites

artículo científico publicado en 2010

A simple procedure for transferring genes cloned in Escherichia coli vectors into other gram-negative bacteria: phenotypic analysis and mapping of TOL plasmid gene xylK.

artículo científico publicado en 1989

Acanthopleuribacter pedis gen. nov., sp. nov., a marine bacterium isolated from a chiton, and description of Acanthopleuribacteraceae fam. nov., Acanthopleuribacterales ord. nov., Holophagaceae fam. nov., Holophagales ord. nov. and Holophagae classi

artículo científico publicado en 2008

Activity and population structure of nitrifying bacteria in an activated-sludge reactor containing polymer beads

artículo científico publicado en 2003

Adhesive protein cDNA sequence of the mussel Mytilus coruscus and its evolutionary implications

artículo científico publicado en 1996

Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria

artículo científico publicado en 2010

Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation

artículo científico publicado en 2003

Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain).

artículo científico publicado en 2011

An AraC/XylS family member at a high level in a hierarchy of regulators for phenol-metabolizing enzymes in Comamonas testosteroni R5

artículo científico publicado en 2002

An effective family shuffling method using single-stranded DNA

artículo científico publicado en 2000

An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol-digesting activated-sludge process.

artículo científico publicado en 1999

Analysis of long-side-chain alkylaromatics in crude oil for evaluation of their fate in the environment

artículo científico publicado en 2001

Artificial evolution by DNA shuffling.

artículo científico publicado en 1998

Bacterial aromatic ring-cleavage enzymes are classified into two different gene families.

artículo científico publicado en 1989

Bacterial populations occuring in a trichloroethylene-contaminated aquifer during methane injection.

artículo científico publicado en 2001

Barnacle cement proteins. Importance of disulfide bonds in their insolubility

artículo científico publicado en 2000

Biochemical and Genetic Characterization of trans -2′-Carboxybenzalpyruvate Hydratase-Aldolase from a Phenanthrene-Degrading Nocardioides Strain ⬇️

artículo científico publicado el 1 de febrero de 1998

Biochemical and Molecular Characterization of 1-Hydroxy-2-naphthoate Dioxygenase from Nocardioides sp. KP7 ⬇️

artículo científico publicado el 3 de abril de 1998

Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7 ⬇️

artículo científico publicado el 1 de octubre de 1997

Biochemical characterization of a Ca(2+)-dependent lectin from the hemolymph of a photosymbiotic marine bivalve, Tridacna derasa (Röding)

scientific article published on 01 May 1995

Biodegradation of n-alkylcycloalkanes and n-alkylbenzenes via new pathways in Alcanivorax sp. strain MBIC 4326.

artículo científico publicado en 2001

Biofilm-based photobioreactor absorbing water and nutrients by capillary action

artículo científico publicado en 2016

Biotransformation of phenanthrene and 1-methoxynaphthalene with Streptomyces lividans cells expressing a marine bacterial phenanthrene dioxygenase gene cluster.

artículo científico publicado en 2001

Characteristics of transposon insertion mutants of mandelic acid-utilizing Pseudomonas putida strain A10L

artículo científico publicado en 1996

Characterization of a high-affinity phenol hydroxylase from Comamonas testosteroni R5 by gene cloning, and expression in Pseudomonas aeruginosa PAO1c

artículo científico publicado en 1999

Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols

artículo científico publicado en 2005

Characterization of genes for enzymes involved in the phenanthrene degradation in Nocardioides sp. KP7.

artículo científico publicado en 1999

Characterization of light-harvesting mutants of Rhodopseudomonas sphaeroides. I. Measurement of the efficiency of energy transfer from light-harvesting complexes to the reaction center

scientific article published on 01 January 1985

Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning.

artículo científico publicado en 1983

Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.

artículo científico publicado en 2004

Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp.

artículo científico publicado en 1998

Cloning of trg, a gene for a sensory transducer in Escherichia coli.

artículo científico publicado en 1982

Cloning, sequencing and sites of expression of genes for the hydroxyarginine-containing adhesive-plaque protein of the mussel Mytilus galloprovincialis

artículo científico publicado en 1996

Cloning, sequencing, and expression of the Pseudomonas testosteroni gene encoding 3-oxosteroid delta 1-dehydrogenase ⬇️

artículo científico publicado el 1 de noviembre de 1991

Codon usage patterns suggest independent evolution of two catabolic operons on toluene-degradative plasmid TOL pWW0 of Pseudomonas putida

artículo científico publicado en 1994

Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution

artículo científico publicado en 1993

Complete genome sequence of the soil actinomycete Kocuria rhizophila

artículo científico publicado en 2008

Conservative Structure of the Plaque Matrix Protein of Mussels in the Genus Mytilus

scientific article published on 01 July 2000

Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System

artículo científico publicado en 2016

Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methylcatechol.

artículo científico publicado en 2004

Construction of the gyrB Database for the Identification and Classification of Bacteria

artículo científico publicado en 1998

Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism.

artículo científico publicado en 1994

Corrosion of iron by iodide-oxidizing bacteria isolated from brine in an iodine production facility

artículo científico publicado en 2014

Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage lambda vehicles

artículo científico publicado en 1981

Crystal ball

artículo científico publicado en 2000

DNA sequence determination of the TOL plasmid (pWW0) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism ⬇️

artículo científico publicado el 1 de octubre de 1991

DNA shuffling and family shuffling for in vitro gene evolution

article

Development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading Alcanivorax strains

artículo científico publicado en 2001

Divergent evolution of chloroplast-type ferredoxins

scientific article published on 01 July 1991

Diversity in kinetics of trichloroethylene-degrading activities exhibited by phenol-degrading bacteria

artículo científico publicado en 2001

Does Tn10 transpose via the cointegrate molecule?

artículo científico publicado en 1984

Effect of motility and chemotaxis on the invasion of Salmonella typhimurium into HeLa cells

artículo científico publicado en 1990

Effects of chloramphenicol on photosynthesis, protein profiles and transketolase activity under extremely high CO2 concentration in an extremely-high-CO2-tolerant green microalga, Chlorococcum littorale

artículo científico publicado en 2004

Energy is required for maturation of exported proteins in Escherichia coli

scientific article published on 01 May 1981

Enhanced mineralization of benzo[a]pyrene in the presence of nonaqueous phase liquids

artículo científico publicado en 2001

Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.

artículo científico publicado en 2009

Evolutionary relationships between catabolic pathways for aromatics: conservation of gene order and nucleotide sequences of catechol oxidation genes of pWW0 and NAH7 plasmids

artículo científico publicado en 1987

Extracellular polysaccharides of Rhodococcus rhodochrous S-2 stimulate the degradation of aromatic components in crude oil by indigenous marine bacteria.

artículo científico publicado en 2002

Functional and evolutionary relationships among diverse oxygenases

artículo científico publicado el 1 de enero de 1992

Functional and structural analyses of trichloroethylene-degrading bacterial communities under different phenol-feeding conditions: laboratory experiments

artículo científico publicado en 2002

Genetic transformation of Pseudochoricystis ellipsoidea, an aliphatic hydrocarbon-producing green alga

artículo científico publicado en 2012

High frequency mobilization of the chromosome of Escherichia coli by a mutant of plasmid RP4 temperature-sensitive for maintenance.

artículo científico publicado en 1980

ICB database: the gyrB database for identification and classification of bacteria.

artículo científico publicado en 2001

Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.

artículo científico publicado en 2009

Identification and characterization of o-xylene-degrading Rhodococcus spp. which were dominant species in the remediation of o-xylene-contaminated soils

artículo científico publicado en 2006

Identification by the phage-display technique of peptides that bind to H7 flagellin of Escherichia coli.

artículo científico publicado en 2003

Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacte

artículo científico publicado en 2009

Induction kinetics of RNA and proteins in exponentially growing organisms

artículo científico publicado en 1991

Influence of extracellular polysaccharides (EPS) produced by two different green unicellular algae on membrane filtration in an algae-based biofuel production process

artículo científico publicado en 2014

Interspecific variations in adhesive protein sequences of Mytilus edulis, M. galloprovincialis, and M. trossulus

artículo científico publicado en 1995

Intersubunit interaction and catalytic activity of catechol 2,3-dioxygenases.

artículo científico publicado en 2003

Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness

artículo científico publicado en 2000

Iron corrosion activity of anaerobic hydrogen-consuming microorganisms isolated from oil facilities

artículo científico publicado en 2010

Iron corrosion induced by nonhydrogenotrophic nitrate-reducing Prolixibacter sp. strain MIC1-1.

artículo científico publicado en 2014

Isolation and characterization of thermophilic bacilli degrading cinnamic, 4-coumaric, and ferulic acids.

artículo científico publicado en 2003

Isolation and phylogenetic characterization of bacteria capable of inducing differentiation in the green alga Monostroma oxyspermum

artículo científico publicado en 2003

Kinetic studies on benzyl alcohol dehydrogenase encoded by TOL plasmid pWWO. A member of the zinc-containing long chain alcohol dehydrogenase family.

artículo científico publicado en 1993

Krokinobacter gen. nov., with three novel species, in the family Flavobacteriaceae.

artículo científico publicado en 2006

Lacticigenium naphtae gen. nov., sp. nov., a halotolerant and motile lactic acid bacterium isolated from crude oil.

artículo científico publicado en 2009

Major Carotenoid Isolated fromParacoccus schoiniaNBRC 100637TIs Adonixanthin Diglucoside

artículo científico publicado en 2006

Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments

artículo científico publicado en 2010

Method for selective isolation of Mycobacteria using olive oil emulsified with SDS

artículo científico publicado en 2007

Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb.

scientific article published on 01 January 2010

Molecular Detection, Isolation, and Physiological Characterization of Functionally Dominant Phenol-Degrading Bacteria in Activated Sludge ⬇️

artículo científico publicado el 1 de noviembre de 1998

Molecular breeding of 2,3-dihydroxybiphenyl 1,2-dioxygenase for enhanced resistance to 3-chlorocatechol

artículo científico publicado en 2004

Molecular characterization and substrate preference of a polycyclic aromatic hydrocarbon dioxygenase from Cycloclasticus sp. strain A5

artículo científico publicado en 2003

Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12 ⬇️

artículo científico publicado el 1 de mayo de 1984

Molecular detection of marine bacterial populations on beaches contaminated by the Nakhodka tanker oil-spill accident.

artículo científico publicado en 2001

Molecular mechanisms of genetic adaptation to xenobiotic compounds ⬇️

artículo científico publicado el 1 de diciembre de 1992

Mutants in transmission of chemotactic signals from two independent receptors of E. coli ⬇️

artículo científico publicado el 1 de marzo de 1979

Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan

artículo científico publicado en 2007

Novel family shuffling methods for the in vitro evolution of enzymes

artículo científico publicado en 1999

Nucleotide and deduced amino acid sequence of the RpoN sigma-factor of Pseudomonas putida.

artículo científico publicado en 1989

Oleibacter marinus gen. nov., sp. nov., a bacterium that degrades petroleum aliphatic hydrocarbons in a tropical marine environment

artículo científico publicado en 2010

Overexpression of the genes from thermophiles in Escherichia coli by high-temperature cultivation.

artículo científico publicado en 2006

Oxygenation reactions of various tricyclic fused aromatic compounds using Escherichia coli and Streptomyces lividans transformants carrying several arene dioxygenase genes.

artículo científico publicado en 2001

PCR isolation of catechol 2,3-dioxygenase gene fragments from environmental samples and their assembly into functional genes.

artículo científico publicado en 1998

Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay.

artículo científico publicado en 2008

Paraferrimonas sedimenticola gen. nov., sp. nov., a marine bacterium of the family Ferrimonadaceae

artículo científico publicado en 2007

Paraoerskovia marina gen. nov., sp. nov., an actinobacterium isolated from marine sediment.

artículo científico publicado en 2009

Petroleum biodegradation in marine environments.

artículo científico publicado en 1999

PhcS represses gratuitous expression of phenol-metabolizing enzymes in Comamonas testosteroni R5.

artículo científico publicado en 2001

Photo-oxidation of biodegraded crude oil and toxicity of the photo-oxidized products.

artículo científico publicado en 2001

Phototactic response of aerobically cultivated Rhodospirillum rubrum

scientific article published on 01 May 1976

Phototaxis and membrane potential in the photosynthetic bacterium Rhodospirillum rubrum ⬇️

artículo científico publicado el 1 de julio de 1977

Phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of Phycisphaeraceae fam. nov., Phycisphaerales ord. nov. and Phycisphaerae classis nov. in the phylum Planctomycetes

artículo científico publicado en 2009

Phylogenetic Analysis of Acinetobacter Strains Based on the Nucleotide Sequences of gyrB Genes and on the Amino Acid Sequences of Their Products

artículo científico publicado en 1996

Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym

artículo científico publicado en 1999

Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes

artículo científico publicado en 1998

Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization

artículo científico publicado en 1999

Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanos...

artículo científico publicado en 2002

Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes.

artículo científico publicado en 2000

Physicochemical characters of a tyrosinase inhibitor produced by Streptomyces roseolilacinus NBRC 12815.

artículo científico publicado en 2009

Population Dynamics of Phenol-Degrading Bacteria in Activated Sludge Determined by gyrB -Targeted Quantitative PCR ⬇️

artículo científico publicado el 1 de abril de 1998

Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate dioxygenases

artículo científico publicado en 1991

Predominant growth of Alcanivorax strains in oil-contaminated and nutrient-supplemented sea water

artículo científico publicado en 2002

Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system

artículo científico publicado en 1991

Proton transfer in benzyl alcohol dehydrogenase during catalysis: alternate proton-relay routes.

artículo científico publicado en 1998

Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts).

artículo científico publicado en 2006

Purification and characterisation of the NADH:acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWW0 of Pseudomonas putida mt-2

artículo científico publicado el 1 de octubre de 1992

Purification and characterization of Acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in Escherichia coli.

artículo científico publicado en 1995

Reclamation of an activated-sludge microbial consortium by selective biostimulation

artículo científico publicado en 2000

Relation of Chemotactic Response to the Amount of Receptor: Evidence for Different Efficiencies of Signal Transduction ⬇️

artículo científico publicado el 1 de junio de 1979

Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous

artículo científico publicado en 2000

Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids.

artículo científico publicado en 2007

Rhodanobacter sp. strain BPC1 in a benzo[a]pyrene-mineralizing bacterial consortium

artículo científico publicado en 2002

Sandarakinotalea sediminis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.

artículo científico publicado en 2006

Sediminicola luteus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae.

artículo científico publicado en 2006

Sediminitomix flava gen. nov., sp. nov., of the phylum Bacteroidetes, isolated from marine sediment.

artículo científico publicado en 2007

Sensory transduction in bacterial chemotaxis.

artículo científico publicado en 1983

Sequential enrichment of microbial populations exhibiting enhanced biodegradation of crude oil.

artículo científico publicado en 1995

Serinibacter salmoneus gen. nov., sp. nov., an actinobacterium isolated from the intestinal tract of a fish, and emended descriptions of the families Beutenbergiaceae and Bogoriellaceae

artículo científico publicado en 2009

Significantly enhanced stability of glucose dehydrogenase by directed evolution.

artículo científico publicado en 2003

Single-stranded DNA family shuffling

artículo científico publicado en 2004

Stable augmentation of activated sludge with foreign catabolic genes harboured by an indigenous dominant bacterium

artículo científico publicado en 2002

Starvation improves survival of bacteria introduced into activated sludge.

artículo científico publicado en 2000

Structural analysis of an acidic, fatty acid ester-bonded extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.

artículo científico publicado en 2007

Structural analysis of an extracellular polysaccharide produced by Rhodococcus rhodochrous strain S-2

artículo científico publicado en 2006

Structural analysis of mucoidan, an acidic extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.

artículo científico publicado en 2007

Structure of the Trg protein: Homologies with and differences from other sensory transducers of Escherichia coli ⬇️

artículo científico publicado el 1 de junio de 1984

Structure of the ring cleavage product of 1-hydroxy-2-naphthoate, an intermediate of the phenanthrene-degradative pathway of Nocardioides sp. strain KP7

artículo científico publicado en 1999

Studies on the export of the maltose-binding protein and the LamB protein

artículo científico publicado en 1982

Substrate specificity differences between two catechol 2,3-dioxygenases encoded by the TOL and NAH plasmids from Pseudomonas putida.

artículo científico publicado en 1995

Substrate specificity of catechol 2,3-dioxygenase encoded by TOL plasmid pWW0 of Pseudomonas putida and its relationship to cell growth

artículo científico publicado en 1994

Substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by TOL plasmid pWW0. Metabolic and mechanistic implications.

artículo científico publicado en 1992

Synthesis of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) with thermostabilized low-specific L-threonine aldolase from Streptomyces coelicolor A3(2).

artículo científico publicado en 2007

Taxonomic distribution of Streptomyces species capable of producing bioactive compounds among strains preserved at NITE/NBRC.

artículo científico publicado en 2008

The E. coli K-12 chromosome flanked by two IS10 sequences transposes

scientific article published on 01 January 1984

The TOL plasmid pWW0 xylN gene product from Pseudomonas putida is involved in m-xylene uptake.

artículo científico publicado en 2001

The bacterivorous soil flagellate Heteromita globosa reduces bacterial clogging under denitrifying conditions in sand-filled aquifer columns.

artículo científico publicado en 2002

The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA.

artículo científico publicado en 2000

The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes.

artículo científico publicado en 1990

The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments

artículo científico publicado en 2010

The soil flagellate Heteromita globosa accelerates bacterial degradation of alkylbenzenes through grazing and acetate excretion in batch culture

artículo científico publicado en 2005

Tn1 insertion mutagenesis in Escherichia coli K-12 using a temperature-sensitive mutant of plasmid RP4

scientific article published on 01 January 1981

Tn501 insertion mutagenesis in Pseudomonas aeruginosa PAO

scientific article published on 01 January 1984

Transposon mutagenesis analysis of meta-cleavage pathway operon genes of the TOL plasmid of Pseudomonas putida mt-2 ⬇️

artículo científico publicado el 1 de octubre de 1984

Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis ⬇️

artículo científico publicado el 20 de marzo de 1979

Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives

artículo científico publicado en 2008

Understanding the diversity in catabolic potential of microorganisms for the development of bioremediation strategies.

artículo científico publicado en 2002

[SWISS-PROT: the curated protein sequence database on Internet]

scientific article published on 01 January 2001

cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily

artículo científico publicado en 1992

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