Lista de obras - Stephen Douthwaite - Dominio Público Uruguay

A "bulged" double helix in a RNA-protein contact site.

artículo científico publicado en 1981

A novel Erm monomethyltransferase in antibiotic-resistant isolates of Mannheimia haemolytica and Pasteurella multocida

artículo científico publicado en 2011

A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25 ⬇️

artículo científico publicado el 11 de junio de 1979

A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA

scientific journal article

Activity of the ketolide telithromycin is refractory to Erm monomethylation of bacterial rRNA.

artículo científico publicado en 2002

Allele-specific priming in the mapping of rRNA

scientific article published on 01 January 1998

Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase

artículo científico publicado en 2007

Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK.

artículo científico publicado en 2013

Basis for prokaryotic specificity of action of aminoglycoside antibiotics

artículo científico publicado en 1999

Binding sites of ribosomal proteins on prokaryotic 5S RNAs: a study with ribonucleases

scientific article published on 01 May 1982

Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni

artículo científico publicado en 2017

Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans

artículo científico publicado en 2017

Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs

artículo científico publicado en 2006

Capreomycin susceptibility is increased by TlyA-directed 2'-O-methylation on both ribosomal subunits

artículo científico publicado en 2012

Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli

artículo científico publicado en 1990

Combinations of Macrolide Resistance Determinants in Field Isolates of Mannheimia haemolytica and Pasteurella multocida ⬇️

artículo científico publicado el 27 de junio de 2011

Comparison of Escherichia coli tRNAPhe in the Free State, in the Ternary Complex and in the Ribosomal A and P Sites by Chemical Probing

scientific article published on 01 March 1983

Complete Genome Sequence of Escherichia coli AS19, an Antibiotic-Sensitive Variant of E. coli Strain B REL606.

artículo científico publicado en 2018

Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs.

artículo científico publicado en 1995

Core sequence in the RNA motif recognized by the ErmE methyltransferase revealed by relaxing the fidelity of the enzyme for its target.

artículo científico publicado en 1999

Crystal structure of the Escherichia coli 23S rRNA:m5C methyltransferase RlmI (YccW) reveals evolutionary links between RNA modification enzymes

artículo científico publicado en 2008

Defining the structural requirements for a helix in 23 S ribosomal RNA that confers erythromycin resistance.

artículo científico publicado en 1989

Design of cAMP-CRP-activated promoters in Escherichia coli.

artículo científico publicado en 1991

Designer drugs for discerning bugs ⬇️

artículo científico publicado el 27 de septiembre de 2010

Does unpaired adenosine-66 from helix II of Escherichia coli 5S RNA bind to protein L18?

artículo científico publicado en 1985

Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase

artículo científico publicado en 1994

Effect of mutations in the A site of 16 S rRNA on aminoglycoside antibiotic-ribosome interaction.

artículo científico publicado en 1999

ErmE methyltransferase recognition elements in RNA substrates 1 1Edited by D. E. Draper

artículo científico publicado en 1998

ErmE methyltransferase recognizes features of the primary and secondary structure in a motif within domain V of 23 S rRNA.

artículo científico publicado en 1999

Erythromycin binding is reduced in ribosomes with conformational alterations in the 23 S rRNA peptidyl transferase loop

artículo científico publicado en 1993

Functional interactions within 23S rRNA involving the peptidyltransferase center.

artículo científico publicado en 1992

Helcococcus kunzii methyltransferase Erm(47) responsible for MLSB resistance is induced by diverse ribosome-targeting antibiotics

artículo científico publicado en 2020

Higher order structure in the 3′-minor domain of small subunit ribosomal RNAs from a gram negative bacterium, a gram positive bacterium and a eukaryote

scientific article published on 01 September 1983

Higher-order structure in the 3′-terminal domain VI of the 23 S ribosomal RNAs from Escherichia coli and Bacillus stearothermophilus

artículo científico publicado en 1984

Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants

artículo científico publicado en 2007

Identifying modifications in RNA by MALDI mass spectrometry

artículo científico publicado en 2007

Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry

artículo científico publicado en 2003

Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger

artículo científico publicado en 2005

Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs

artículo científico publicado en 2013

Inhibition of protein synthesis on the ribosome by tildipirosin compared with other veterinary macrolides

artículo científico publicado en 2012

Integrative and Conjugative Elements (ICEs) in Pasteurellaceae Species and Their Detection by Multiplex PCR.

artículo científico publicado en 2018

Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I.

artículo científico publicado en 2008

Interconversion of active and inactive 30 S ribosomal subunits is accompanied by a conformational change in the decoding region of 16 S rRNA.

artículo científico publicado en 1986

Isolation of temperature-sensitive mutants of 16 S rRNA in Escherichia coli.

artículo científico publicado en 1989

Ketolide antimicrobial activity persists after disruption of interactions with domain II of 23S rRNA.

artículo científico publicado en 2004

Ketolide resistance in Streptococcus pyogenes correlates with the degree of rRNA dimethylation by Erm.

artículo científico publicado en 2005

LNAzymes: incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage.

artículo científico publicado en 2002

Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets

artículo científico publicado en 2006

Macrolide resistance by ribosomal mutation in clinical isolates of Streptococcus pneumoniae from the PROTEKT 1999-2000 study

artículo científico publicado en 2003

Macrolide resistance conferred by base substitutions in 23S rRNA.

artículo científico publicado en 2001

Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida

scientific article published on 25 September 2014

Macrolide-ketolide inhibition of MLS-resistant ribosomes is improved by alternative drug interaction with domain II of 23S rRNA

artículo científico publicado en 2000

Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria

artículo científico publicado en 2002

Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase

artículo científico publicado en 2004

Methyltransferase Erm(37) slips on rRNA to confer atypical resistance in Mycobacterium tuberculosis

artículo científico publicado en 2005

Minimal substrate features for Erm methyltransferases defined by using a combinatorial oligonucleotide library.

artículo científico publicado en 2011

Molecular mechanisms by which rRNA mutations confer resistance to clindamycin

artículo científico publicado en 2005

Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida ⬇️

artículo científico publicado el 7 de mayo de 2012

Mutations in conserved helix 69 of 23S rRNA of Thermus thermophilus that affect capreomycin resistance but not posttranscriptional modifications

artículo científico publicado en 2008

Mutations in domain II of 23 S rRNA facilitate translation of a 23 S rRNA-encoded pentapeptide conferring erythromycin resistance

artículo científico publicado en 1996

Mycobacterium smegmatis Erm(38) is a reluctant dimethyltransferase

artículo científico publicado en 2005

Negative in vitro selection identifies the rRNA recognition motif for ErmE methyltransferase

artículo científico publicado en 1999

Overexpression of the mitochondrial methyltransferase TFB1M in the mouse does not impact mitoribosomal methylation status or hearing

artículo científico publicado en 2015

Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action.

artículo científico publicado en 2000

Pseudoknot in domain II of 23 S rRNA is essential for ribosome function

artículo científico publicado en 1995

Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II).

artículo científico publicado en 2007

Reductive Evolution and Diversification of C5-Uracil Methylation in the Nucleic Acids of Mollicutes

scientific article published on 10 April 2020

Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides

artículo científico publicado en 2015

Resistance to the macrolide antibiotic tylosin is conferred by single methylations at 23S rRNA nucleotides G748 and A2058 acting in synergy

artículo científico publicado en 2002

Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre.

artículo científico publicado en 1993

Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases

scientific article published on 01 December 1981

Specific structural probing of plasmid-coded ribosomal RNAs from Escherichia coli

artículo científico publicado en 1991

Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases.

artículo científico publicado en 2010

Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII.

artículo científico publicado en 2003

Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA

artículo científico publicado en 2012

Structure-activity relationships of ketolides vs. macrolides

artículo científico publicado en 2001

Telithromycin resistance in Streptococcus pneumoniae is conferred by a deletion in the leader sequence of erm(B) that increases rRNA methylation

artículo científico publicado en 2007

The activity of rRNA resistance methyltransferases assessed by MALDI mass spectrometry

artículo científico publicado en 2008

The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site

artículo científico publicado en 2009

The antibiotics micrococcin and thiostrepton interact directly with 23S rRNA nucleotides 1067A and 1095A.

artículo científico publicado en 1994

The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose

artículo científico publicado en 2003

The bacterial ribosome as a target for antibiotics

artículo científico publicado en 2005

The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in Mycoplasma capricolum 23S rRNA.

artículo científico publicado en 2014

The ketolides: a critical review

artículo científico publicado en 2002

The macrolide binding site on the bacterial ribosome

artículo científico publicado en 2002

The macrolide-ketolide antibiotic binding site is formed by structures in domains II and V of 23S ribosomal RNA.

artículo científico publicado en 1999

The ribosomal S4-RNA fragment melts cooperatively when complexed with protein S4 ⬇️

artículo científico publicado el 1 de abril de 1979

The structural basis of macrolide-ribosome binding assessed using mutagenesis of 23S rRNA positions 2058 and 2059.

artículo científico publicado en 2004

The structure of the RNA m5C methyltransferase YebU from Escherichia coli reveals a C-terminal RNA-recruiting PUA domain.

artículo científico publicado en 2006

The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA. ⬇️

artículo científico publicado en 2000

The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748.

artículo científico publicado en 2004

Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon

artículo científico publicado en 1994

Visualizing the 16-membered ring macrolides tildipirosin and tilmicosin bound to their ribosomal site.

artículo científico publicado en 2012

YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA.

artículo científico publicado en 2008

YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962.

artículo científico publicado en 2008

YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407.

artículo científico publicado en 2006

YgdE is the 2'-O-ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA.

artículo científico publicado en 2009

YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors

artículo científico publicado en 2010

Lista de obras de Stephen Douthwaite

A "bulged" double helix in a RNA-protein contact site.

A novel Erm monomethyltransferase in antibiotic-resistant isolates of Mannheimia haemolytica and Pasteurella multocida

A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25 ⬇️

A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA

Activity of the ketolide telithromycin is refractory to Erm monomethylation of bacterial rRNA.

Allele-specific priming in the mapping of rRNA

Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase

Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK.

Basis for prokaryotic specificity of action of aminoglycoside antibiotics

Binding sites of ribosomal proteins on prokaryotic 5S RNAs: a study with ribonucleases

Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni

Bypassing rRNA methylation by RsmA/Dim1during ribosome maturation in the hyperthermophilic archaeon Nanoarchaeum equitans

Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs

Capreomycin susceptibility is increased by TlyA-directed 2'-O-methylation on both ribosomal subunits

Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli

Combinations of Macrolide Resistance Determinants in Field Isolates of Mannheimia haemolytica and Pasteurella multocida ⬇️

Comparison of Escherichia coli tRNAPhe in the Free State, in the Ternary Complex and in the Ribosomal A and P Sites by Chemical Probing

Complete Genome Sequence of Escherichia coli AS19, an Antibiotic-Sensitive Variant of E. coli Strain B REL606.

Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs.

Core sequence in the RNA motif recognized by the ErmE methyltransferase revealed by relaxing the fidelity of the enzyme for its target.

Crystal structure of the Escherichia coli 23S rRNA:m5C methyltransferase RlmI (YccW) reveals evolutionary links between RNA modification enzymes

Defining the structural requirements for a helix in 23 S ribosomal RNA that confers erythromycin resistance.

Design of cAMP-CRP-activated promoters in Escherichia coli.

Designer drugs for discerning bugs ⬇️

Does unpaired adenosine-66 from helix II of Escherichia coli 5S RNA bind to protein L18?

Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase

Effect of mutations in the A site of 16 S rRNA on aminoglycoside antibiotic-ribosome interaction.

ErmE methyltransferase recognition elements in RNA substrates 1 1Edited by D. E. Draper

ErmE methyltransferase recognizes features of the primary and secondary structure in a motif within domain V of 23 S rRNA.

Erythromycin binding is reduced in ribosomes with conformational alterations in the 23 S rRNA peptidyl transferase loop

Functional interactions within 23S rRNA involving the peptidyltransferase center.

Helcococcus kunzii methyltransferase Erm(47) responsible for MLSB resistance is induced by diverse ribosome-targeting antibiotics

Higher order structure in the 3′-minor domain of small subunit ribosomal RNAs from a gram negative bacterium, a gram positive bacterium and a eukaryote

Higher-order structure in the 3′-terminal domain VI of the 23 S ribosomal RNAs from Escherichia coli and Bacillus stearothermophilus

Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants

Identifying modifications in RNA by MALDI mass spectrometry

Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry

Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger

Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs

Inhibition of protein synthesis on the ribosome by tildipirosin compared with other veterinary macrolides

Integrative and Conjugative Elements (ICEs) in Pasteurellaceae Species and Their Detection by Multiplex PCR.

Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I.

Interconversion of active and inactive 30 S ribosomal subunits is accompanied by a conformational change in the decoding region of 16 S rRNA.

Isolation of temperature-sensitive mutants of 16 S rRNA in Escherichia coli.

Ketolide antimicrobial activity persists after disruption of interactions with domain II of 23S rRNA.

Ketolide resistance in Streptococcus pyogenes correlates with the degree of rRNA dimethylation by Erm.

LNAzymes: incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage.

Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets

Macrolide resistance by ribosomal mutation in clinical isolates of Streptococcus pneumoniae from the PROTEKT 1999-2000 study

Macrolide resistance conferred by base substitutions in 23S rRNA.

Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida

Macrolide-ketolide inhibition of MLS-resistant ribosomes is improved by alternative drug interaction with domain II of 23S rRNA

Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria

Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase

Methyltransferase Erm(37) slips on rRNA to confer atypical resistance in Mycobacterium tuberculosis

Minimal substrate features for Erm methyltransferases defined by using a combinatorial oligonucleotide library.

Molecular mechanisms by which rRNA mutations confer resistance to clindamycin

Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida ⬇️

Mutations in conserved helix 69 of 23S rRNA of Thermus thermophilus that affect capreomycin resistance but not posttranscriptional modifications

Mutations in domain II of 23 S rRNA facilitate translation of a 23 S rRNA-encoded pentapeptide conferring erythromycin resistance

Mycobacterium smegmatis Erm(38) is a reluctant dimethyltransferase

Negative in vitro selection identifies the rRNA recognition motif for ErmE methyltransferase

Overexpression of the mitochondrial methyltransferase TFB1M in the mouse does not impact mitoribosomal methylation status or hearing

Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action.

Pseudoknot in domain II of 23 S rRNA is essential for ribosome function

Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II).

Reductive Evolution and Diversification of C5-Uracil Methylation in the Nucleic Acids of Mollicutes

Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides

Resistance to the macrolide antibiotic tylosin is conferred by single methylations at 23S rRNA nucleotides G748 and A2058 acting in synergy

Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre.

Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases

Specific structural probing of plasmid-coded ribosomal RNAs from Escherichia coli

Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases.

Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII.

Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA

Structure-activity relationships of ketolides vs. macrolides

Telithromycin resistance in Streptococcus pneumoniae is conferred by a deletion in the leader sequence of erm(B) that increases rRNA methylation

The activity of rRNA resistance methyltransferases assessed by MALDI mass spectrometry

The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site