Lista de obras - Richard John Ward - Dominio Público Uruguay

A SequenceSpace analysis of Lys49 phopholipases A2: clues towards identification of residues involved in a novel mechanism of membrane damage and in myotoxicity

artículo científico publicado en 1998

A designed bifunctional laccase/β-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse

artículo científico publicado en 2012

A micelle nucleation model for the interaction of dodecyl sulphate with Lys49-phospholipases A(2).

artículo científico publicado en 2006

A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change.

artículo científico publicado en 2004

A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

artículo científico publicado en 2014

A pH-induced dissociation of the dimeric form of a lysine 49-phospholipase A2 abolishes Ca2+-independent membrane damaging activity

artículo científico publicado en 2001

A practical teaching course in directed protein evolution using the green fluorescent protein as a model.

artículo científico publicado en 2011

A predominant role for hydrogen bonding in the stability of the homodimer of bothropstoxin-I, A lysine 49-phospholipase A2.

artículo científico publicado en 2005

A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus

scientific article published on 01 October 1999

A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination

artículo científico publicado en 2016

Activation of Ca2+-independent membrane-damaging activity in Lys49-phospholipase A2 promoted by amphiphilic molecules

artículo científico publicado en 2004

Active site mutants of human secreted Group IIA Phospholipase A2 lacking hydrolytic activity retain their bactericidal effect

scientific article published on 06 October 2011

Amino acid sequence of a myotoxic Lys49-phospholipase A2 homologue from the venom of Cerrophidion (Bothrops) godmani

artículo científico publicado en 1998

An ultraviolet photoacoustic spectroscopy study of the interaction between Lys49-phospholipase A2 and amphiphilic molecules

artículo científico publicado en 2006

At the interface: crystal structures of phospholipases A2

artículo científico publicado en 1998

Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.

artículo científico publicado en 2015

Biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris

artículo científico publicado en 2014

Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni.

artículo científico publicado en 2010

Characterization of suramin binding sites on the human group IIA secreted phospholipase A2 by site-directed mutagenesis and molecular dynamics simulation

artículo científico publicado en 2012

Characterization of temperature dependent and substrate-binding cleft movements in Bacillus circulans family 11 xylanase: a molecular dynamics investigation.

artículo científico publicado en 2009

Chemical denaturation of a homodimeric lysine-49 phospholipase A2: a stable dimer interface and a native monomeric intermediate

artículo científico publicado en 2003

Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme

artículo científico publicado en 2014

Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis ⬇️

artículo científico publicado el 23 de julio de 2011

Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1)

artículo científico publicado en 2005

Crystal structure of piratoxin-I: a calcium-independent, myotoxic phospholipase A2-homologue from Bothrops pirajai venom

artículo científico publicado en 1998

Crystallization and preliminary X-ray crystallographic studies of the mesophilic xylanase A from Bacillus subtilis 1A1.

artículo científico publicado en 2005

Crystallization and preliminary diffraction data of bothropstoxin I isolated from the venom of Bothrops jararacussu ⬇️

artículo científico publicado el 1 de marzo de 1995

Crystallization of bothropstoxin II isolated from the venom of Bothrops jararacussu

scientific article published on 01 May 1996

Crystallization of piratoxin I, a myotoxic Lys49-phospholipase A2 homologue isolated from the venom of Bothrops pirajai.

artículo científico publicado en 1998

D-xylose detection in Escherichia coli by a xylose binding protein-dependent response

artículo científico publicado en 2013

Defective T-cell activation by Mycoplasma arthritidis mitogen is restored by interferon-gamma

artículo científico publicado en 1989

Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168

artículo científico publicado en 2012

Dissociation of enzymatic and pharmacological properties of piratoxins-I and -III, two myotoxic phospholipases A2 from Bothrops pirajai snake venom

artículo científico publicado en 2001

Effect of crotapotin on the biological activity of Asp49 and Lys49 phospholipases A(2) from Bothrops snake venoms

artículo científico publicado en 2004

Efficient constitutive expression of Bacillus subtilis xylanase A in Escherichia coli DH5alpha under the control of the Bacillus BsXA promoter

artículo científico publicado en 2006

Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.

artículo científico publicado en 2011

Engineering the pattern of protein glycosylation modulates the thermostability of a GH11 xylanase.

artículo científico publicado en 2013

Enhanced xyloglucan-specific endo-β-1,4-glucanase efficiency in an engineered CBM44-XegA chimera

artículo científico publicado en 2015

Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: potentiation of hemolytic activity by LipL32.

artículo científico publicado en 2005

Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major

artículo científico publicado en 2006

Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1,4-glucanase (GH12) from Aspergillus niveus

artículo científico publicado en 2012

GH53 Endo-Beta-1,4-Galactanase from a Newly Isolated Bacillus licheniformis CBMAI 1609 as an Enzymatic Cocktail Supplement for Biomass Saccharification

artículo científico publicado en 2016

Identification of a putative membrane-inserted segment in the alpha-toxin of Staphylococcus aureus

artículo científico publicado en 1994

Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis.

artículo científico publicado en 2015

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

artículo científico publicado en 2015

Insights on calcium-independent phospholipid membrane damage by Lys49-PLA2 using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu

artículo científico publicado en 2007

Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa.

artículo científico publicado en 2003

Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance.

artículo científico publicado en 2017

Lipid bilayer stabilization of the Na,K-ATPase reconstituted in DPPC/DPPE liposomes

scientific article published on 01 January 2006

Mapping of suramin binding sites on the group IIA human secreted phospholipase A2.

artículo científico publicado en 2009

Mapping of the structural determinants of artificial and biological membrane damaging activities of a Lys49 phospholipase A2 by scanning alanine mutagenesis

scientific article published on 09 February 2007

Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis.

artículo científico publicado en 2003

Method for forming two-dimensional paracrystals of biological filaments on lipid monolayers

artículo científico publicado en 1990

Molecular adaptability of nucleoside diphosphate kinase b from trypanosomatid parasites: stability, oligomerization and structural determinants of nucleotide binding

artículo científico publicado en 2011

Molecular modeling of the human eukaryotic translation initiation factor 5A (eIF5A) based on spectroscopic and computational analyses

artículo científico publicado en 2006

Ontogenetic changes in Phoneutria nigriventer (Araneae, Ctenidae) spider venom

artículo científico publicado en 2004

Permeabilization of E. coli K12 inner and outer membranes by bothropstoxin-I, A LYS49 phospholipase A2 from Bothrops jararacussu

artículo científico publicado en 2007

Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

artículo científico publicado en 2009

Properties of a purified thermostable glucoamylase from Aspergillus niveus.

artículo científico publicado en 2009

Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus

artículo científico publicado en 2009

Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii

artículo científico publicado en 2007

Purification and characterization of two xylanases from alkalophilic and thermophilic Bacillus licheniformis 77-2.

artículo científico publicado en 2006

Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin.

artículo científico publicado en 2002

Reduced pH induces an inactive non-native conformation of the monomeric bothropstoxin-I (Lys49-PLA2).

artículo científico publicado en 2009

Reflex changes in respiration and heart rate evoked by intravenous and left ventricular injection of 5-HT and capsaicin in anaesthetized rats: a comparison of mechanisms

artículo científico publicado en 1984

Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli

artículo científico publicado en 2009

Sequence of a cDNA encoding bothropstoxin I, a myotoxin from the venom of Bothrops jararacussu

artículo científico publicado en 1995

Shared structural determinants for the calcium-independent liposome membrane permeabilization and sarcolemma depolarization in Bothropstoxin-I, a LYS49-PLA(2) from the venom of Bothrops jararacussu.

artículo científico publicado en 2009

Spectroscopic characterization and structural modeling of prolamin from maize and pearl millet

artículo científico publicado en 2003

Structural and functional analysis of BmjMIP, a phospholipase A2 myotoxin inhibitor protein from Bothrops moojeni snake plasma

artículo científico publicado en 2003

Structural basis for glucose tolerance in GH1 β-glucosidases

artículo científico publicado en 2014

Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid

scientific article published on 01 February 2005

Structure of a Lys49-Phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)

scientific article published on 01 February 1999

Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom

artículo científico publicado en 1995

Subproteomic analysis of soluble proteins of the microsomal fraction from two Leishmania species

artículo científico publicado en 2006

Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening

artículo científico publicado en 2014

Tertiary structural changes of the alpha-hemolysin from Staphylococcus aureus on association with liposome membranes

scientific article published on 01 March 1998

The Staphylococcus aureus alpha-toxin channel complex and the effect of Ca2+ ions on its interaction with lipid layers

artículo científico publicado en 1992

The amino acid sequence of ribitol dehydrogenase-F, a mutant enzyme with improved xylitol dehydrogenase activity

artículo científico publicado en 1999

The association of Na,K-ATPase subunits studied by circular dichroism, surface tension and dilatational elasticity

scientific article published on 13 June 2008

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities

artículo científico publicado en 2012

The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata.

artículo científico publicado en 2004

The effect of resonance energy homotransfer on the intrinsic tryptophan fluorescence emission of the bothropstoxin-I dimer

artículo científico publicado en 2001

The interaction of bothropstoxin-I (Lys49-PLA(2)) with liposome membranes.

artículo científico publicado en 2009

Thermostabilization of Bacillus subtilis GH11 xylanase by surface charge engineering

artículo científico publicado en 2016

Thermostable variants of the recombinant xylanase A from Bacillus subtilis produced by directed evolution show reduced heat capacity changes

artículo científico publicado en 2008

Lista de obras de Richard John Ward

A SequenceSpace analysis of Lys49 phopholipases A2: clues towards identification of residues involved in a novel mechanism of membrane damage and in myotoxicity

A designed bifunctional laccase/β-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse

A micelle nucleation model for the interaction of dodecyl sulphate with Lys49-phospholipases A(2).

A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change.

A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

A pH-induced dissociation of the dimeric form of a lysine 49-phospholipase A2 abolishes Ca2+-independent membrane damaging activity

A practical teaching course in directed protein evolution using the green fluorescent protein as a model.

A predominant role for hydrogen bonding in the stability of the homodimer of bothropstoxin-I, A lysine 49-phospholipase A2.

A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus

A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination

Activation of Ca2+-independent membrane-damaging activity in Lys49-phospholipase A2 promoted by amphiphilic molecules

Active site mutants of human secreted Group IIA Phospholipase A2 lacking hydrolytic activity retain their bactericidal effect

Amino acid sequence of a myotoxic Lys49-phospholipase A2 homologue from the venom of Cerrophidion (Bothrops) godmani

An ultraviolet photoacoustic spectroscopy study of the interaction between Lys49-phospholipase A2 and amphiphilic molecules

At the interface: crystal structures of phospholipases A2

Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.

Biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris

Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni.

Characterization of suramin binding sites on the human group IIA secreted phospholipase A2 by site-directed mutagenesis and molecular dynamics simulation

Characterization of temperature dependent and substrate-binding cleft movements in Bacillus circulans family 11 xylanase: a molecular dynamics investigation.

Chemical denaturation of a homodimeric lysine-49 phospholipase A2: a stable dimer interface and a native monomeric intermediate

Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme

Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis ⬇️

Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1)

Crystal structure of piratoxin-I: a calcium-independent, myotoxic phospholipase A2-homologue from Bothrops pirajai venom

Crystallization and preliminary X-ray crystallographic studies of the mesophilic xylanase A from Bacillus subtilis 1A1.

Crystallization and preliminary diffraction data of bothropstoxin I isolated from the venom of Bothrops jararacussu ⬇️

Crystallization of bothropstoxin II isolated from the venom of Bothrops jararacussu

Crystallization of piratoxin I, a myotoxic Lys49-phospholipase A2 homologue isolated from the venom of Bothrops pirajai.

D-xylose detection in Escherichia coli by a xylose binding protein-dependent response

Defective T-cell activation by Mycoplasma arthritidis mitogen is restored by interferon-gamma

Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168

Dissociation of enzymatic and pharmacological properties of piratoxins-I and -III, two myotoxic phospholipases A2 from Bothrops pirajai snake venom

Effect of crotapotin on the biological activity of Asp49 and Lys49 phospholipases A(2) from Bothrops snake venoms

Efficient constitutive expression of Bacillus subtilis xylanase A in Escherichia coli DH5alpha under the control of the Bacillus BsXA promoter

Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.

Engineering the pattern of protein glycosylation modulates the thermostability of a GH11 xylanase.

Enhanced xyloglucan-specific endo-β-1,4-glucanase efficiency in an engineered CBM44-XegA chimera

Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: potentiation of hemolytic activity by LipL32.

Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major

Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1,4-glucanase (GH12) from Aspergillus niveus

GH53 Endo-Beta-1,4-Galactanase from a Newly Isolated Bacillus licheniformis CBMAI 1609 as an Enzymatic Cocktail Supplement for Biomass Saccharification

Identification of a putative membrane-inserted segment in the alpha-toxin of Staphylococcus aureus

Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis.

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

Insights on calcium-independent phospholipid membrane damage by Lys49-PLA2 using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu

Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa.

Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance.

Lipid bilayer stabilization of the Na,K-ATPase reconstituted in DPPC/DPPE liposomes

Mapping of suramin binding sites on the group IIA human secreted phospholipase A2.

Mapping of the structural determinants of artificial and biological membrane damaging activities of a Lys49 phospholipase A2 by scanning alanine mutagenesis

Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis.

Method for forming two-dimensional paracrystals of biological filaments on lipid monolayers

Molecular adaptability of nucleoside diphosphate kinase b from trypanosomatid parasites: stability, oligomerization and structural determinants of nucleotide binding

Molecular modeling of the human eukaryotic translation initiation factor 5A (eIF5A) based on spectroscopic and computational analyses

Ontogenetic changes in Phoneutria nigriventer (Araneae, Ctenidae) spider venom

Permeabilization of E. coli K12 inner and outer membranes by bothropstoxin-I, A LYS49 phospholipase A2 from Bothrops jararacussu

Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

Properties of a purified thermostable glucoamylase from Aspergillus niveus.

Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus

Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii

Purification and characterization of two xylanases from alkalophilic and thermophilic Bacillus licheniformis 77-2.

Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin.

Reduced pH induces an inactive non-native conformation of the monomeric bothropstoxin-I (Lys49-PLA2).

Reflex changes in respiration and heart rate evoked by intravenous and left ventricular injection of 5-HT and capsaicin in anaesthetized rats: a comparison of mechanisms

Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli

Sequence of a cDNA encoding bothropstoxin I, a myotoxin from the venom of Bothrops jararacussu

Shared structural determinants for the calcium-independent liposome membrane permeabilization and sarcolemma depolarization in Bothropstoxin-I, a LYS49-PLA(2) from the venom of Bothrops jararacussu.

Spectroscopic characterization and structural modeling of prolamin from maize and pearl millet

Structural and functional analysis of BmjMIP, a phospholipase A2 myotoxin inhibitor protein from Bothrops moojeni snake plasma

Structural basis for glucose tolerance in GH1 β-glucosidases

Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid

Structure of a Lys49-Phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)

Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom

Subproteomic analysis of soluble proteins of the microsomal fraction from two Leishmania species

Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening

Tertiary structural changes of the alpha-hemolysin from Staphylococcus aureus on association with liposome membranes

The Staphylococcus aureus alpha-toxin channel complex and the effect of Ca2+ ions on its interaction with lipid layers

The amino acid sequence of ribitol dehydrogenase-F, a mutant enzyme with improved xylitol dehydrogenase activity