Lista de obras - Jim Huggett - Dominio Público Uruguay

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

artículo científico publicado en 2013

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

artículo científico publicado en 2014

Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

artículo científico

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

artículo científico publicado en 2016

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

artículo científico publicado en 2012

Assessing the accuracy of quantitative molecular microbial profiling

artículo científico publicado en 2014

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine

scientific article published on 14 June 2018

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

scientific article published on 03 April 2019

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

artículo científico publicado en 2014

Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.

artículo científico publicado en 2014

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

artículo científico publicado en 2012

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

artículo científico publicado en 2014

Considerations for digital PCR as an accurate molecular diagnostic tool.

artículo científico publicado en 2014

Crohn's disease and MAP.

artículo científico publicado en 2004

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

artículo científico publicado en 2015

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

artículo científico publicado en 2018

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis

artículo científico publicado en 2010

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

artículo científico publicado en 2008

Digital PCR as a novel technology and its potential implications for molecular diagnostics

artículo científico publicado en 2013

Digital PCR, a technique for the future

artículo científico publicado en 2016

Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study

scientific article published on 12 February 2020

Evaluation of digital PCR for absolute DNA quantification

artículo científico publicado en 2011

Evaluation of digital PCR for absolute RNA quantification

artículo científico publicado en 2013

Expression of a novel cytokine, IL-4delta2, in HIV and HIV-tuberculosis co-infection.

artículo científico publicado en 2005

Expression of apoptosis-related genes in an Ethiopian cohort study correlates with tuberculosis clinical status

artículo científico publicado en 2010

Fundamentals of multiplexing with digital PCR

artículo científico publicado en 2016

Future diagnosis of sepsis – Authors' reply

artículo científico publicado en 2010

G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon

artículo científico publicado en 2017

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

artículo científico publicado en 2015

How to make Mathematics Biology's next and better microscope.

artículo científico publicado en 2014

Implications of storing urinary DNA from different populations for molecular analyses

artículo científico publicado en 2009

Improving the standardization of mRNA measurement by RT-qPCR.

artículo científico publicado en 2018

In vivo and in vitro studies of a novel cytokine, interleukin 4delta2, in pulmonary tuberculosis

artículo científico publicado en 2005

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

artículo científico publicado en 2017

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs

scientific article published on 24 June 2019

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

artículo científico publicado en 2017

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

artículo científico publicado en 2016

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

artículo científico publicado en 2016

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons

artículo científico publicado en 2015

Low sensitivity of a urine LAM-ELISA in the diagnosis of pulmonary tuberculosis

artículo científico publicado en 2009

Lung remodeling in pulmonary tuberculosis

artículo científico publicado en 2005

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

artículo científico publicado en 2010

Making standards for quantitative real-time pneumococcal PCR.

scientific article published on 04 December 2014

Methods for applying accurate digital PCR analysis on low copy DNA samples

artículo científico publicado en 2013

Minimum information necessary for quantitative real-time PCR experiments.

artículo científico publicado en 2014

Myobacterium tuberculosis induces selective up-regulation of TLRs in the mononuclear leukocytes of patients with active pulmonary tuberculosis

artículo científico publicado en 2006

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.

artículo científico publicado en 2016

Nucleic acid detection and quantification in the developing world

artículo científico publicado en 2009

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells

artículo científico publicado en 2013

Pneumocystis jirovecii in pleural infection: a nucleic acid amplification study

artículo científico publicado en 2010

Primer sequence disclosure: a clarification of the MIQE guidelines.

artículo científico publicado en 2011

Problems of developing molecular diagnostic tests for opportunistic pathogens: the example of Pneumocystis jirovecii.

artículo científico publicado en 2006

Progress in metagenomics requires a balanced appraisal of the available technologies.

artículo científico publicado en 2013

Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis

artículo científico publicado en 2012

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

artículo científico publicado en 2014

Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis

artículo científico publicado en 2019

RNA reference materials with defined viral RNA loads of SARS-CoV-2-A useful tool towards a better PCR assay harmonization

artículo científico publicado en 2022

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia

artículo científico publicado en 2016

Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study

artículo científico publicado en 2011

Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods

scientific article published on August 2009

Real-time RT-PCR normalisation; strategies and considerations.

artículo científico publicado en 2005

Reflections on the white plague

artículo científico publicado en 2009

Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing

artículo científico publicado en 2020

Reproducibility of biomedical research - The importance of editorial vigilance

artículo científico publicado en 2017

Response to the Letter from Garcia-Montojo and colleagues concerning our paper entitled, Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotroph

scientific article published on 03 July 2019

Seasonal variation in mortality of Pneumocystis jirovecii pneumonia in HIV-infected patients

article

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL.

artículo científico publicado en 2016

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

artículo científico publicado en 2014

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

artículo científico publicado en 2009

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

artículo científico publicado en 2013

The need for transparency and good practices in the qPCR literature.

artículo científico publicado en 2013

The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain.

artículo científico publicado en 2000

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis

artículo científico publicado en 2007

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis

artículo científico publicado en 2016

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

artículo científico publicado en 2014

Trials and tribulations of an African-led research and capacity development programme: the case for EDCTP investments

artículo científico publicado en 2010

Tuberculosis: amplification-based clinical diagnostic techniques

artículo científico publicado en 2003

Type 2 cytokines in respiratory syncytial virus bronchiolitis

artículo científico publicado en 2004

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

artículo científico publicado en 2009

Utility of the antigen-specific interferon-gamma assay for the management of tuberculosis

artículo científico publicado en 2005

Variation in gamma interferon responses to different infecting strains of Mycobacterium tuberculosis in acid-fast bacillus smear-positive patients and household contacts in Antananarivo, Madagascar

artículo científico publicado en 2010

qPCR primer design revisited

artículo científico publicado en 2017

qPCR, dPCR, NGS - A journey.

artículo científico publicado en 2015

Lista de obras de Jim Huggett

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

Assessing the accuracy of quantitative molecular microbial profiling

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine

Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river

Clinical features, microbiology and surgical outcomes of infective endocarditis: a 13-year study from a UK tertiary cardiothoracic referral centre.

Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Considerations for digital PCR as an accurate molecular diagnostic tool.

Crohn's disease and MAP.

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

Digital PCR as a novel technology and its potential implications for molecular diagnostics

Digital PCR, a technique for the future

Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study

Evaluation of digital PCR for absolute DNA quantification

Evaluation of digital PCR for absolute RNA quantification

Expression of a novel cytokine, IL-4delta2, in HIV and HIV-tuberculosis co-infection.

Expression of apoptosis-related genes in an Ethiopian cohort study correlates with tuberculosis clinical status

Fundamentals of multiplexing with digital PCR

Future diagnosis of sepsis – Authors' reply

G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

How to make Mathematics Biology's next and better microscope.

Implications of storing urinary DNA from different populations for molecular analyses

Improving the standardization of mRNA measurement by RT-qPCR.

In vivo and in vitro studies of a novel cytokine, interleukin 4delta2, in pulmonary tuberculosis

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction.

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons

Low sensitivity of a urine LAM-ELISA in the diagnosis of pulmonary tuberculosis

Lung remodeling in pulmonary tuberculosis

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Making standards for quantitative real-time pneumococcal PCR.

Methods for applying accurate digital PCR analysis on low copy DNA samples

Minimum information necessary for quantitative real-time PCR experiments.

Myobacterium tuberculosis induces selective up-regulation of TLRs in the mononuclear leukocytes of patients with active pulmonary tuberculosis

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic Myeloid Leukemia Patients.

Nucleic acid detection and quantification in the developing world

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac₂SGL complexes from Mycobacterium tuberculosis-infected cells

Pneumocystis jirovecii in pleural infection: a nucleic acid amplification study

Primer sequence disclosure: a clarification of the MIQE guidelines.

Problems of developing molecular diagnostic tests for opportunistic pathogens: the example of Pneumocystis jirovecii.

Progress in metagenomics requires a balanced appraisal of the available technologies.

Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis

RNA reference materials with defined viral RNA loads of SARS-CoV-2-A useful tool towards a better PCR assay harmonization

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia

Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study

Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods

Real-time RT-PCR normalisation; strategies and considerations.

Reflections on the white plague

Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing

Reproducibility of biomedical research - The importance of editorial vigilance

Response to the Letter from Garcia-Montojo and colleagues concerning our paper entitled, Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotroph

Seasonal variation in mortality of Pneumocystis jirovecii pneumonia in HIV-infected patients

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL.

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

The need for transparency and good practices in the qPCR literature.

The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain.

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

Trials and tribulations of an African-led research and capacity development programme: the case for EDCTP investments

Tuberculosis: amplification-based clinical diagnostic techniques

Type 2 cytokines in respiratory syncytial virus bronchiolitis

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.