Lista de obras - Helmut Schwab - Dominio Público Uruguay

(S)-Hydroxynitrile Lyase from Hevea brasiliensis

article

A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase

artículo científico publicado en 2008

Alternative pig liver esterase (APLE) - cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst

artículo científico publicado en 2007

An efficient plasmid vector for expression cloning of large numbers of PCR fragments in Escherichia coli.

artículo científico publicado en 2007

Analysis of the multimer resolution system encoded by the parCBA operon of broad-host-range plasmid RP4.

artículo científico publicado en 1994

Biocatalytic Friedel-Crafts alkylation using non-natural cofactors

artículo científico publicado en 2009

Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase

artículo científico publicado en 2013

C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli

artículo científico publicado en 2010

Cell surface expression of bacterial esterase A by Saccharomyces cerevisiae and its enhancement by constitutive activation of the cellular unfolded protein response.

artículo científico publicado en 2006

Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis.

artículo científico publicado en 2013

Characterization of two bacterial hydroxynitrile lyases with high similarity to cupin superfamily proteins

artículo científico publicado en 2012

Cloning and characterization of the gene for the thermostable xylanase XynA from Thermomyces lanuginosus.

artículo científico publicado en 1996

Cloning and sequence analysis of Mucor circinelloides glyceraldehyde-3-phosphate dehydrogenase gene.

artículo científico publicado en 2002

Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis

artículo científico publicado en 1994

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

artículo científico publicado en 2015

Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis.

artículo científico publicado en 2003

Crystal Structure of an (R)-Selective ω-Transaminase from Aspergillus terreus

artículo científico publicado en 2014

Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis.

artículo científico publicado en 1996

Crystallization and preliminary X-ray diffraction studies of the Pseudomonas marginata esterase EstB

artículo científico publicado en 1997

Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis

artículo científico publicado en 2012

Detection of a new enzyme for stereoselective hydrolysis of linalyl acetate using simple plate assays for the characterization of cloned esterases from Burkholderia gladioli

artículo científico publicado en 1998

Discovery of a novel (R)-selective bacterial hydroxynitrile lyase from Acidobacterium capsulatum

artículo científico publicado en 2014

Efficient secretion of bacillus subtilis levanase by saccharomyces cerevisiae

artículo científico publicado en 1995

Endophytic Pseudomonas spp. populations of pathogen-infected potato plants analysed by 16S rDNA- and 16S rRNA-based denaturating gradient gel electrophoresis

article

Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents

artículo científico publicado en 2010

Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida

Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis.

artículo científico publicado en 2004

Esterase EstE from Xanthomonas vesicatoria ( Xv_EstE) is an outer membrane protein capable of hydrolyzing long-chain polar esters.

artículo científico publicado en 2003

Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members

artículo científico publicado en 2020

Expression of Bacillus subtilis levanase gene in Lactobacillus plantarum and Lactobacillus casei

artículo científico

Expression of the Bacillus subtilis levanase gene in Escherichia coli and Saccharomyces cerevisiae.

artículo científico publicado en 1991

Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli

artículo científico publicado en 2011

Fusion of binding domains to Thermobifida cellulosilytica cutinase to tune sorption characteristics and enhancing PET hydrolysis.

artículo científico publicado en 2013

Heterologous expression and characterization of choline oxidase from the soil bacterium Arthrobacter nicotianae

artículo científico publicado en 2008

High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.

artículo científico publicado en 1997

High-quality genome sequence of Pichia pastoris CBS7435

High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

artículo científico publicado en 2014

Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis

artículo científico publicado en 2011

Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations

artículo científico publicado en 2014

Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4'-oxocyclohexyl)acetonitrile as the model.

artículo científico publicado en 2008

Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage

artículo científico publicado en 2012

Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed and designed evolution

artículo científico publicado en 2006

Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis

artículo científico publicado en 1996

Mechanistic study of CMP-Neu5Ac hydrolysis by α2,3-sialyltransferase from Pasteurella dagmatis

artículo científico publicado en 2014

Metagenome analyses reveal the influence of the inoculant Lactobacillus buchneri CD034 on the microbial community involved in grass ensiling

artículo científico publicado en 2013

Metagenomic analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) operon of an uncultured bacterial endophyte colonizing Solanum tuberosum L.

artículo científico publicado en 2011

Micro-colony array based high throughput platform for enzyme library screening

artículo científico publicado en 2006

Molecular characterization and functional analysis in Aspergillus nidulans of the 5'-region of the Penicillium chrysogenum isopenicillin N synthetase gene

artículo científico publicado el 1 de enero de 1991

Molecular cloning and homology modeling of protocatechuate 3,4-dioxygenase from Pseudomonas marginata.

artículo científico publicado en 1996

Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis. Functional expression in Escherichia coli and Saccharomyces cerevisiae and identification of an active site residue.

artículo científico publicado en 1996

Molecular cloning, sequencing and expression in Escherichia coli of the poly(3-hydroxyalkanoate) synthesis genes from Alcaligenes latus DSM1124.

artículo científico publicado en 1998

Nitrile hydrolysis activity of Rhodococcus erythropolis NCIMB 11540 whole cells

artículo científico publicado en 2006

Nucleotide sequence of a cloned 2.5 kb PstI--EcoRI Bacillus subtilis DNA fragment coding for levanase

artículo científico publicado en 1987

Observation of a short, strong hydrogen bond in the active site of hydroxynitrile lyase from Hevea brasiliensis explains a large pKa shift of the catalytic base induced by the reaction intermediate

artículo científico publicado en 2003

Occurrence of deletion plasmids at high rates after conjugative transfer of the plasmids RP4 and RK2 from Escherichia coli to Alcaligenes eutrophus H16.

artículo científico publicado en 1983

Over-expression of ICE2 stabilizes cytochrome P450 reductase in Saccharomyces cerevisiae and Pichia pastoris.

artículo científico publicado en 2015

Partitioning of broad-host-range plasmid RP4 is a complex system involving site-specific recombination.

artículo científico publicado en 1990

Plasmid RK2 ParB protein: purification and nuclease properties.

artículo científico publicado en 1999

Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase

artículo científico publicado en 1997

Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris

artículo científico publicado en 2014

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.

artículo científico

Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli ⬇️

artículo científico publicado el 1 de mayo de 1995

Random strand transfer recombination (RSTR) for homology-independent nucleic acid recombination

artículo científico publicado en 2006

Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering

artículo científico publicado en 2013

Recent advances in rational approaches for enzyme engineering

artículo científico publicado en 2012

Reprint of "versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16"

artículo científico publicado en 2014

Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp. atroseptica

artículo científico publicado en 2002

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

artículo científico publicado en 2015

Special issue: enzyme technology and biocatalysis

artículo científico publicado en 2006

Stability and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution and structural interpretation of muteins

artículo científico publicado en 2006

Stability of r-microbes: stabilization of plasmid vectors by the partitioning function of broad-host-range plasmid RP4.

artículo científico

Structure of the xylanase from Penicillium simplicissimum

artículo científico publicado en 1998

Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis

artículo científico publicado en 2013

The ParB protein encoded by the RP4 par region is a Ca(2+)-dependent nuclease linearizing circular DNA substrates

artículo científico publicado en 1997

The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity

scientific journal article

The defense-related rice gene Pir7b encodes an alpha/beta hydrolase fold protein exhibiting esterase activity towards naphthol AS-esters

artículo científico publicado en 1998

The divergent promoters mediating transcription of the par locus of plasmid RP4 are subject to autoregulation.

artículo científico publicado en 1992

Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene

artículo científico publicado en 1988

Versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16.

artículo científico publicado en 2014

Versatile plasmid-based expression systems for Gram-negative bacteria--General essentials exemplified with the bacterium Ralstonia eutropha H16.

artículo científico

Lista de obras de Helmut Schwab

(S)-Hydroxynitrile Lyase from Hevea brasiliensis

A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase

Alternative pig liver esterase (APLE) - cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst

An efficient plasmid vector for expression cloning of large numbers of PCR fragments in Escherichia coli.

Analysis of the multimer resolution system encoded by the parCBA operon of broad-host-range plasmid RP4.

Biocatalytic Friedel-Crafts alkylation using non-natural cofactors

Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase

C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli

Cell surface expression of bacterial esterase A by Saccharomyces cerevisiae and its enhancement by constitutive activation of the cellular unfolded protein response.

Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis.

Characterization of two bacterial hydroxynitrile lyases with high similarity to cupin superfamily proteins

Cloning and characterization of the gene for the thermostable xylanase XynA from Thermomyces lanuginosus.

Cloning and sequence analysis of Mucor circinelloides glyceraldehyde-3-phosphate dehydrogenase gene.

Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis.

Crystal Structure of an (R)-Selective ω-Transaminase from Aspergillus terreus

Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis.

Crystallization and preliminary X-ray diffraction studies of the Pseudomonas marginata esterase EstB

Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis

Detection of a new enzyme for stereoselective hydrolysis of linalyl acetate using simple plate assays for the characterization of cloned esterases from Burkholderia gladioli

Discovery of a novel (R)-selective bacterial hydroxynitrile lyase from Acidobacterium capsulatum

Efficient secretion of bacillus subtilis levanase by saccharomyces cerevisiae

Endophytic Pseudomonas spp. populations of pathogen-infected potato plants analysed by 16S rDNA- and 16S rRNA-based denaturating gradient gel electrophoresis

Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents

Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida

Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis.

Esterase EstE from Xanthomonas vesicatoria ( Xv_EstE) is an outer membrane protein capable of hydrolyzing long-chain polar esters.

Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members

Expression of Bacillus subtilis levanase gene in Lactobacillus plantarum and Lactobacillus casei

Expression of the Bacillus subtilis levanase gene in Escherichia coli and Saccharomyces cerevisiae.

Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli

Fusion of binding domains to Thermobifida cellulosilytica cutinase to tune sorption characteristics and enhancing PET hydrolysis.

Heterologous expression and characterization of choline oxidase from the soil bacterium Arthrobacter nicotianae

High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.

High-quality genome sequence of Pichia pastoris CBS7435

High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis

Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations

Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4'-oxocyclohexyl)acetonitrile as the model.

Insights into the completely annotated genome of Lactobacillus buchneri CD034, a strain isolated from stable grass silage

Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed and designed evolution

Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis

Mechanistic study of CMP-Neu5Ac hydrolysis by α2,3-sialyltransferase from Pasteurella dagmatis

Metagenome analyses reveal the influence of the inoculant Lactobacillus buchneri CD034 on the microbial community involved in grass ensiling

Metagenomic analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) operon of an uncultured bacterial endophyte colonizing Solanum tuberosum L.

Micro-colony array based high throughput platform for enzyme library screening

Molecular characterization and functional analysis in Aspergillus nidulans of the 5'-region of the Penicillium chrysogenum isopenicillin N synthetase gene

Molecular cloning and homology modeling of protocatechuate 3,4-dioxygenase from Pseudomonas marginata.

Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis. Functional expression in Escherichia coli and Saccharomyces cerevisiae and identification of an active site residue.

Molecular cloning, sequencing and expression in Escherichia coli of the poly(3-hydroxyalkanoate) synthesis genes from Alcaligenes latus DSM1124.

Nitrile hydrolysis activity of Rhodococcus erythropolis NCIMB 11540 whole cells

Nucleotide sequence of a cloned 2.5 kb PstI--EcoRI Bacillus subtilis DNA fragment coding for levanase

Observation of a short, strong hydrogen bond in the active site of hydroxynitrile lyase from Hevea brasiliensis explains a large pKa shift of the catalytic base induced by the reaction intermediate

Occurrence of deletion plasmids at high rates after conjugative transfer of the plasmids RP4 and RK2 from Escherichia coli to Alcaligenes eutrophus H16.

Over-expression of ICE2 stabilizes cytochrome P450 reductase in Saccharomyces cerevisiae and Pichia pastoris.

Partitioning of broad-host-range plasmid RP4 is a complex system involving site-specific recombination.

Plasmid RK2 ParB protein: purification and nuclease properties.

Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase

Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.

Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli ⬇️

Random strand transfer recombination (RSTR) for homology-independent nucleic acid recombination

Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering

Recent advances in rational approaches for enzyme engineering

Reprint of "versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16"

Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp. atroseptica

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Special issue: enzyme technology and biocatalysis

Stability and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution and structural interpretation of muteins

Stability of r-microbes: stabilization of plasmid vectors by the partitioning function of broad-host-range plasmid RP4.

Structure of the xylanase from Penicillium simplicissimum

Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis

The ParB protein encoded by the RP4 par region is a Ca(2+)-dependent nuclease linearizing circular DNA substrates

The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity

The defense-related rice gene Pir7b encodes an alpha/beta hydrolase fold protein exhibiting esterase activity towards naphthol AS-esters

The divergent promoters mediating transcription of the par locus of plasmid RP4 are subject to autoregulation.

Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene

Versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16.