Lista de obras - Boyke Bunk - Dominio Público Uruguay

A Clostridioides difficile bacteriophage genome encodes functional binary toxin-associated genes

artículo científico publicado en 2017

A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity

artículo científico publicado en 2020

A publicly accessible database for Clostridioides difficile genome sequences supports tracing of transmission chains and epidemics

artículo científico publicado en 2020

A short story about a big magic bug.

artículo científico publicado en 2010

A transferable plasticity region in Campylobacter coli allows isolates of an otherwise non-glycolytic food-borne pathogen to catabolize glucose

artículo científico publicado en 2015

BacDive – The Bacterial Diversity Metadatabase in 2016

research article

BacDive – the Bacterial Diversity Metadatabase

research article

Caldisalinibacter kiritimatiensisgen. nov., sp. nov., a Moderately Thermohalophilic Thiosulfate-Reducing Bacterium from a Hypersaline Microbial Mat

article

Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy).

artículo científico publicado en 2015

Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae

artículo científico publicado en 2017

Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions.

artículo científico publicado en 2010

Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum

artículo científico publicado en 2016

Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria

artículo científico publicado en 2016

Chromera velia, endosymbioses and the rhodoplex hypothesis--plastid evolution in cryptophytes, alveolates, stramenopiles, and haptophytes (CASH lineages)

artículo científico publicado en 2014

Chromosomal Locations of mcr-1 and bla CTX-M-15 in Fluoroquinolone-Resistant Escherichia coli ST410.

artículo científico publicado en 2016

Cobaviruses – a new globally distributed phage group infecting Rhodobacteraceae in marine ecosystems

artículo científico publicado en 2019

Comparative Genomics Reveal a Flagellar System, a Type VI Secretion System and Plant Growth-Promoting Gene Clusters Unique to the Endophytic Bacterium

artículo científico publicado en 2018

Comparative Transcriptomic Profiling of Yersinia enterocolitica O:3 and O:8 Reveals Major Expression Differences of Fitness- and Virulence-Relevant Genes Indicating Ecological Separation

article

Comparative genome and phenotypic analysis of three Clostridioides difficile strains isolated from a single patient provide insight into multiple infection of C. difficile

artículo científico publicado en 2018

Comparative genomic analysis of eight novel haloalkaliphilic bacteriophages from Lake Elmenteita, Kenya

article

Complete Genome Sequence of Highly Adherent Pseudomonas aeruginosa Small-Colony Variant SCV20265

artículo científico publicado en 2014

Complete Genome Sequence of Kyrpidia sp. Strain EA-1, a Thermophilic Knallgas Bacterium, Isolated from the Azores

artículo científico publicado en 2018

Complete Genome Sequence of a CTX-M-15-Producing Klebsiella pneumoniae Outbreak Strain from Multilocus Sequence Type 514.

artículo científico publicado en 2015

Complete Genome Sequence of blaCTX-M-27-Encoding Escherichia coli Strain H105 of Sequence Type 131 Lineage C1/H30R.

artículo científico publicado en 2017

Complete Genome Sequence of the Barley Pathogen Xanthomonas translucens pv. translucens DSM 18974T (ATCC 19319T).

artículo científico publicado en 2016

Complete Genome Sequence of the Clostridium difficile Type Strain DSM 1296T.

artículo científico publicado en 2015

Complete Genome Sequence of the Novel Temperate Clostridium difficile Phage phiCDIF1296T.

artículo científico publicado en 2015

Complete Genome Sequence of the Silicimonas algicola Type Strain, a Representative of the Marine Group Isolated from the Cell Surface of the Marine Diatom Thalassiosira delicatula

Complete Genome Sequence of the Urethral Catheter Isolate Myroides sp. A21.

artículo científico publicado en 2015

Complete Genome Sequences of Three Multidrug-Resistant Clinical Isolates of Streptococcus pneumoniae Serotype 19A with Different Susceptibilities to the Myxobacterial Metabolite Carolacton

artículo científico publicado en 2017

Complete Genome and Plasmid Sequences of Staphylococcus aureus EDCC 5055 (DSM 28763), Used To Study Implant-Associated Infections

artículo científico publicado en 2017

Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246.

artículo científico publicado en 2017

Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat

artículo científico publicado en 2015

Complete genome sequence of the bioleaching bacterium Leptospirillum sp. group II strain CF-1.

artículo científico publicado en 2016

Construction and characterization of nitrate and nitrite respiring Pseudomonas putida KT2440 strains for anoxic biotechnical applications

artículo científico publicado en 2012

Convergent Loss of ABC Transporter Genes From Clostridioides difficile Genomes Is Associated With Impaired Tyrosine Uptake and p-Cresol Production.

artículo científico publicado en 2018

Designing the MIRRI information system

Detection of translocatable units in a blaCTX-M-15 extended-spectrum β-lactamase-producing ST131 Escherichia coli isolate using a hybrid sequencing approach

artículo científico publicado en 2016

Divergent evolution in the genomes of closely related lacertids, Lacerta viridis and L. bilineata, and implications for speciation

Diversity of prokaryotic community at a shallow marine hydrothermal site elucidated by Illumina sequencing technology

artículo científico publicado en 2014

Enhanced whole exome sequencing by higher DNA insert lengths.

artículo científico publicado en 2016

Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection

artículo científico publicado en 2018

First Complete Genome Sequence of a Subdivision 6 Acidobacterium Strain

artículo científico publicado en 2016

First Report of Kosakonia radicincitans Bacteraemia from Europe (Austria) - Identification and Whole-Genome Sequencing of Strain DSM 107547

artículo científico publicado en 2020

First genome sequences of Achromobacter phages reveal new members of the N4 family

artículo científico publicado en 2014

Fuerstia marisgermanicae gen. nov., sp. nov., an Unusual Member of the Phylum Planctomycetes from the German Wadden Sea.

artículo científico publicado en 2016

GeneReporter--sequence-based document retrieval and annotation

artículo científico publicado en 2011

Genome Analysis of the Carbapenem- and Colistin-Resistant Escherichia coli Isolate NRZ14408 Reveals Horizontal Gene Transfer Pathways towards Panresistance and Enhanced Virulence

artículo científico publicado en 2017

Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis

artículo científico publicado en 2016

Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630.

artículo científico publicado en 2015

Genome Sequence of Escherichia coli E28, a Multidrug-Resistant Strain Isolated from a Chicken Carcass, and Its Spontaneously Inducible Prophage

artículo científico publicado en 2017

Genome Sequence of Prosthecochloris sp. Strain CIB 2401 of the Phylum Chlorobi

artículo científico publicado en 2016

Genome Sequence of the Small-Colony Variant Pseudomonas aeruginosa MH27, Isolated from a Chronic Urethral Catheter Infection

artículo científico publicado en 2014

Genome and Methylome Variation in Helicobacter pylori With a cag Pathogenicity Island During Early Stages of Human Infection

artículo científico publicado en 2017

Genome biology of a novel lineage of planctomycetes widespread in anoxic aquatic environments

artículo científico publicado en 2018

Genome sequence of the mud-dwelling archaeon Methanoplanus limicola type strain (DSM 2279(T)), reclassification of Methanoplanus petrolearius as Methanolacinia petrolearia and emended descriptions of the genera Methanoplanus and Methanolacinia

artículo científico publicado en 2014

Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.

artículo científico publicado en 2011

Genome-guided design of a defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium

artículo científico publicado en 2016

Genomic and transcriptomic characterization of Pseudomonas aeruginosa small colony variants derived from a chronic infection model

artículo científico publicado en 2019

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

artículo científico publicado en 2016

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

artículo científico publicado en 2017

Global outbreak of severe Mycobacterium chimaera disease after cardiac surgery: a molecular epidemiological study

artículo científico

High metabolic versatility of different toxigenic and non-toxigenic Clostridioides difficile isolates

artículo científico publicado en 2017

High-Quality Whole-Genome Sequences of the Oligo-Mouse-Microbiota Bacterial Community

artículo científico publicado en 2017

Host Specificity for Bacterial, Archaeal and Fungal Communities Determined for High- and Low-Microbial Abundance Sponge Species in Two Genera

artículo científico publicado en 2017

Identification and targeted cultivation of abundant novel freshwater sphingomonads and analysis of their population substructure

artículo científico publicado en 2011

Identification of a Pseudomonas aeruginosa PAO1 DNA Methyltransferase, Its Targets, and Physiological Roles.

artículo científico publicado en 2017

Impact of rare codons and the functional coproduction of rate-limiting tRNAs on recombinant protein production in Bacillus megaterium.

artículo científico publicado en 2015

Inactivation of farR Causes High Rhodomyrtone Resistance and Increased Pathogenicity in Staphylococcus aureus.

artículo científico publicado en 2019

Isolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya

article

Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia

artículo científico publicado en 2018

Long-read DNA metabarcoding of ribosomal RNA in the analysis of fungi from aquatic environments

artículo científico publicado en 2018

Manual curation and reannotation of the genomes of Clostridium difficile 630Δerm and C. difficile 630.

artículo científico publicado en 2017

MetaQuant: a tool for the automatic quantification of GC/MS-based metabolome data

artículo científico publicado en 2006

Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium

artículo científico publicado en 2009

Molecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testing

artículo científico publicado en 2021

Monitoring microevolution of OXA-48-producing Klebsiella pneumoniae ST147 in a hospital setting by SMRT sequencing

artículo científico publicado en 2017

Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation

scientific article published in 2022

Optimization of antibody fragment production in Bacillus megaterium: the role of metal ions on protein secretion.

artículo científico publicado en 2010

OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica.

artículo científico publicado en 2015

PRODORIC (release 2009): a database and tool platform for the analysis of gene regulation in prokaryotes

artículo científico publicado en 2008

Packaging of Dinoroseobacter shibae DNA into Gene Transfer Agent Particles Is Not Random

artículo científico publicado en 2018

Paenibacillus larvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

artículo científico publicado en 2015

Production of the Bioactive Compounds Violacein and Indolmycin Is Conditional in a maeA Mutant of Pseudoalteromonas luteoviolacea S4054 Lacking the Malic Enzyme

artículo científico publicado en 2016

ROSY--a flexible and universal database and bioinformatics tool platform for Roseobacter related species

artículo científico publicado en 2008

Rapid and Consistent Evolution of Colistin Resistance in Extensively Drug-Resistant Pseudomonas aeruginosa during Morbidostat Culture

artículo científico publicado en 2017

Rapid and consistent evolution of colistin resistance in Pseudomonas aeruginosa during morbidostat culture

article

SYSTOMONAS--an integrated database for systems biology analysis of Pseudomonas

artículo científico publicado en 2007

Sequencing and characterization of Pseudomonas aeruginosa phage JG004.

artículo científico publicado en 2011

Synchronous termination of replication of the two chromosomes is an evolutionary selected feature in Vibrionaceae

artículo científico publicado en 2018

Systems biology of recombinant protein production in Bacillus megaterium

artículo científico publicado en 2010

Systems biology of recombinant protein production using Bacillus megaterium

artículo científico publicado en 2011

The Biofilm Inhibitor Carolacton Enters Gram-Negative Cells: Studies Using a TolC-Deficient Strain of Escherichia coli

artículo científico publicado en 2017

The Composite 259-kb Plasmid of Martelella mediterranea DSM 17316T-A Natural Replicon with Functional RepABC Modules from Rhodobacteraceae and Rhizobiaceae

artículo científico publicado en 2017

The Global Catalogue of Microorganisms 10K type strain sequencing project: closing the genomic gaps for the validly published prokaryotic and fungi species

artículo científico publicado en 2018

The Global Genome Biodiversity Network (GGBN) Data Standard specification

artículo científico publicado en 2016

The Mycobacterium avium ssp. paratuberculosis specific mptD gene is required for maintenance of the metabolic homeostasis necessary for full virulence in mouse infections

artículo científico publicado en 2014

The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea.

artículo científico publicado en 2009

The complex methylome of the human gastric pathogen Helicobacter pylori

artículo científico publicado en 2013

The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host

artículo científico publicado en 2022

Think pink: photosynthesis, plasmids and the Roseobacter clade

artículo científico publicado en 2012

Thiorhodovibrio frisius and Trv. litoralis spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities

artículo científico publicado en 2023

Trajectories and Drivers of Genome Evolution in Surface-Associated Marine Phaeobacter

artículo científico publicado en 2017

Transcriptional adaptations during long-term persistence of Staphylococcus aureus in the airways of a cystic fibrosis patient

artículo científico publicado en 2014

Transcriptional response of the photoheterotrophic marine bacterium Dinoroseobacter shibae to changing light regimes

artículo científico publicado en 2011

Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria

artículo científico publicado en 2017

Trophic networks improve the performance of microbial anodes treating wastewater

scientific article published on 27 September 2019

Two new Rhizobiales species isolated from root nodules of common sainfoin ( Onobrychis viciifolia ) show different plant colonization strategies

artículo científico publicado en 2022

Vibrio anguillarum Is Genetically and Phenotypically Unaffected by Long-Term Continuous Exposure to the Antibacterial Compound Tropodithietic Acid

artículo científico publicado en 2016

Vibrio syngnathi sp. nov., a fish pathogen, isolated from the Kiel Fjord

scientific article published in 2023

Whole-genome comparison of high and low virulent Staphylococcus aureus isolates inducing implant-associated bone infections

artículo científico publicado en 2018

Whole-genome sequencing of a large collection of Myroides odoratimimus and Myroides odoratus isolates and antimicrobial susceptibility studies

artículo científico publicado en 2018

Lista de obras de Boyke Bunk

A Clostridioides difficile bacteriophage genome encodes functional binary toxin-associated genes

A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity

A publicly accessible database for Clostridioides difficile genome sequences supports tracing of transmission chains and epidemics

A short story about a big magic bug.

A transferable plasticity region in Campylobacter coli allows isolates of an otherwise non-glycolytic food-borne pathogen to catabolize glucose

BacDive – The Bacterial Diversity Metadatabase in 2016

BacDive – the Bacterial Diversity Metadatabase

Caldisalinibacter kiritimatiensisgen. nov., sp. nov., a Moderately Thermohalophilic Thiosulfate-Reducing Bacterium from a Hypersaline Microbial Mat

Changes in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy).

Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae

Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions.

Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum

Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria

Chromera velia, endosymbioses and the rhodoplex hypothesis--plastid evolution in cryptophytes, alveolates, stramenopiles, and haptophytes (CASH lineages)

Chromosomal Locations of mcr-1 and bla CTX-M-15 in Fluoroquinolone-Resistant Escherichia coli ST410.

Cobaviruses – a new globally distributed phage group infecting Rhodobacteraceae in marine ecosystems

Comparative Genomics Reveal a Flagellar System, a Type VI Secretion System and Plant Growth-Promoting Gene Clusters Unique to the Endophytic Bacterium

Comparative Transcriptomic Profiling of Yersinia enterocolitica O:3 and O:8 Reveals Major Expression Differences of Fitness- and Virulence-Relevant Genes Indicating Ecological Separation

Comparative genome and phenotypic analysis of three Clostridioides difficile strains isolated from a single patient provide insight into multiple infection of C. difficile

Comparative genomic analysis of eight novel haloalkaliphilic bacteriophages from Lake Elmenteita, Kenya

Complete Genome Sequence of Highly Adherent Pseudomonas aeruginosa Small-Colony Variant SCV20265

Complete Genome Sequence of Kyrpidia sp. Strain EA-1, a Thermophilic Knallgas Bacterium, Isolated from the Azores

Complete Genome Sequence of a CTX-M-15-Producing Klebsiella pneumoniae Outbreak Strain from Multilocus Sequence Type 514.

Complete Genome Sequence of blaCTX-M-27-Encoding Escherichia coli Strain H105 of Sequence Type 131 Lineage C1/H30R.

Complete Genome Sequence of the Barley Pathogen Xanthomonas translucens pv. translucens DSM 18974T (ATCC 19319T).

Complete Genome Sequence of the Clostridium difficile Type Strain DSM 1296T.

Complete Genome Sequence of the Novel Temperate Clostridium difficile Phage phiCDIF1296T.

Complete Genome Sequence of the Silicimonas algicola Type Strain, a Representative of the Marine Group Isolated from the Cell Surface of the Marine Diatom Thalassiosira delicatula

Complete Genome Sequence of the Urethral Catheter Isolate Myroides sp. A21.

Complete Genome Sequences of Three Multidrug-Resistant Clinical Isolates of Streptococcus pneumoniae Serotype 19A with Different Susceptibilities to the Myxobacterial Metabolite Carolacton

Complete Genome and Plasmid Sequences of Staphylococcus aureus EDCC 5055 (DSM 28763), Used To Study Implant-Associated Infections

Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246.

Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat

Complete genome sequence of the bioleaching bacterium Leptospirillum sp. group II strain CF-1.

Construction and characterization of nitrate and nitrite respiring Pseudomonas putida KT2440 strains for anoxic biotechnical applications

Convergent Loss of ABC Transporter Genes From Clostridioides difficile Genomes Is Associated With Impaired Tyrosine Uptake and p-Cresol Production.

Designing the MIRRI information system

Detection of translocatable units in a blaCTX-M-15 extended-spectrum β-lactamase-producing ST131 Escherichia coli isolate using a hybrid sequencing approach

Divergent evolution in the genomes of closely related lacertids, Lacerta viridis and L. bilineata, and implications for speciation

Diversity of prokaryotic community at a shallow marine hydrothermal site elucidated by Illumina sequencing technology

Enhanced whole exome sequencing by higher DNA insert lengths.

Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection

First Complete Genome Sequence of a Subdivision 6 Acidobacterium Strain

First Report of Kosakonia radicincitans Bacteraemia from Europe (Austria) - Identification and Whole-Genome Sequencing of Strain DSM 107547

First genome sequences of Achromobacter phages reveal new members of the N4 family

Fuerstia marisgermanicae gen. nov., sp. nov., an Unusual Member of the Phylum Planctomycetes from the German Wadden Sea.

GeneReporter--sequence-based document retrieval and annotation

Genome Analysis of the Carbapenem- and Colistin-Resistant Escherichia coli Isolate NRZ14408 Reveals Horizontal Gene Transfer Pathways towards Panresistance and Enhanced Virulence

Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis

Genome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630.

Genome Sequence of Escherichia coli E28, a Multidrug-Resistant Strain Isolated from a Chicken Carcass, and Its Spontaneously Inducible Prophage

Genome Sequence of Prosthecochloris sp. Strain CIB 2401 of the Phylum Chlorobi

Genome Sequence of the Small-Colony Variant Pseudomonas aeruginosa MH27, Isolated from a Chronic Urethral Catheter Infection

Genome and Methylome Variation in Helicobacter pylori With a cag Pathogenicity Island During Early Stages of Human Infection

Genome biology of a novel lineage of planctomycetes widespread in anoxic aquatic environments

Genome sequence of the mud-dwelling archaeon Methanoplanus limicola type strain (DSM 2279(T)), reclassification of Methanoplanus petrolearius as Methanolacinia petrolearia and emended descriptions of the genera Methanoplanus and Methanolacinia

Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.

Genome-guided design of a defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium

Genomic and transcriptomic characterization of Pseudomonas aeruginosa small colony variants derived from a chronic infection model

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

Global occurrence and heterogeneity of the Roseobacter-clade species Ruegeria mobilis

Global outbreak of severe Mycobacterium chimaera disease after cardiac surgery: a molecular epidemiological study

High metabolic versatility of different toxigenic and non-toxigenic Clostridioides difficile isolates

High-Quality Whole-Genome Sequences of the Oligo-Mouse-Microbiota Bacterial Community

Host Specificity for Bacterial, Archaeal and Fungal Communities Determined for High- and Low-Microbial Abundance Sponge Species in Two Genera

Identification and targeted cultivation of abundant novel freshwater sphingomonads and analysis of their population substructure

Identification of a Pseudomonas aeruginosa PAO1 DNA Methyltransferase, Its Targets, and Physiological Roles.

Impact of rare codons and the functional coproduction of rate-limiting tRNAs on recombinant protein production in Bacillus megaterium.

Inactivation of farR Causes High Rhodomyrtone Resistance and Increased Pathogenicity in Staphylococcus aureus.

Isolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya

Kyrpidia spormannii sp. nov., a thermophilic, hydrogen-oxidizing, facultative autotroph, isolated from hydrothermal systems at São Miguel Island, and emended description of the genus Kyrpidia

Long-read DNA metabarcoding of ribosomal RNA in the analysis of fungi from aquatic environments

Manual curation and reannotation of the genomes of Clostridium difficile 630Δerm and C. difficile 630.

MetaQuant: a tool for the automatic quantification of GC/MS-based metabolome data

Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium

Molecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testing

Monitoring microevolution of OXA-48-producing Klebsiella pneumoniae ST147 in a hospital setting by SMRT sequencing

Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation

Optimization of antibody fragment production in Bacillus megaterium: the role of metal ions on protein secretion.