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Lista de obras de Wolfgang Hillen

A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10.

artículo científico publicado en 1985

A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.

artículo científico publicado en 1994

A gene regulation system with four distinct expression levels

artículo científico publicado en 2006

A new strategy for the analysis of tetracycline residues in foodstuffs by a surface plasmon resonance biosensor

artículo científico publicado en 2006

A novel method to analyze nucleocytoplasmic transport in vivo by using short peptide tags

artículo científico publicado en 2013

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

artículo científico publicado en 2002

A novel tetracycline-controlled transactivator-transrepressor system enables external control of oncolytic adenovirus replication

artículo científico publicado en 2003

A peptide triggers allostery in tet repressor by binding to a unique site

artículo científico publicado en 2005

A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR

artículo científico publicado en 2008

A short autonomous repression motif is located within the N-terminal domain of CTCF.

artículo científico publicado en 2004

A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo

artículo científico publicado en 2004

A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.

artículo científico publicado en 1988

Acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion in Escherichia coli

artículo científico publicado en 1986

Activity reversal of Tet repressor caused by single amino acid exchanges

artículo científico publicado en 2004

Adjusting transgene expression levels in lymphocytes with a set of inducible promoters

artículo científico publicado en 2010

Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.

artículo científico publicado en 1998

Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor ⬇️

artículo científico publicado el 1 de diciembre de 1991

An RNA aptamer that induces transcription

artículo científico publicado en 2009

An accurate method for determining the helical repeat of DNA in solution reveals differences to the crystal structures of two B-DNA decamers

artículo científico publicado en 1992

An exclusive α/β code directs allostery in TetR-peptide complexes

artículo científico publicado en 2012

An optimized conditional suicide switch using doxycycline-dependent expression of human tBid

artículo científico publicado en 2005

Analysis of tet operator-TET repressor complexes by thermal denaturation studies.

artículo científico publicado en 1982

Anhydrotetracycline-peptide conjugates as representatives for ligand-based transactivating systems

artículo científico publicado en 2010

Backbone dynamics of Tet repressor alpha8intersectionalpha9 loop.

artículo científico publicado en 2000

Carbon source control of the phosphorylation state of the Bacillus subtilis carbon-flux regulator Crh in vivo.

artículo científico publicado en 2011

CcpA forms complexes with CodY and RpoA in Bacillus subtilis

scientific journal article

CcpA mutants with differential activities in Bacillus subtilis

artículo científico publicado en 2007

Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction

scientific article published on 01 August 1995

Click-chemistry-derived tetracycline-amino acid conjugates exhibiting exceptional potency and exclusive recognition of the reverse tet repressor

artículo científico publicado en 2010

Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA.

artículo científico publicado en 2004

Complementation of a Delta ccpA mutant of Lactobacillus casei with CcpA mutants affected in the DNA- and cofactor-binding domains.

artículo científico publicado en 2004

Complex formation between malate dehydrogenase and isocitrate dehydrogenase from Bacillus subtilis is regulated by tricarboxylic acid cycle metabolites

scientific journal article

Computer simulations and experimental studies of gel mobility patterns for weak and strong non-cooperative protein binding to two targets on the same DNA: application to binding of tet repressor variants to multiple and single tet operator sites

artículo científico publicado en 1991

Conditional cell suicide using dox‐dependent caspase‐2 expression ⬇️

artículo científico publicado el 1 de abril de 2003

Conditional gene expression by controlling translation with tetracycline-binding aptamers

artículo científico publicado en 2003

Conformational changes induced in the Tet repressor protein TetR(B) upon operator or anhydrotetracycline binding as revealed by time-resolved fluorescence spectroscopy on single tryptophan mutants.

artículo científico publicado en 2000

Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation

artículo científico publicado en 1998

Conformational changes of the Tet repressor induced by tetracycline trapping

artículo científico publicado en 1998

Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene

artículo científico publicado en 1987

Contacts between Tet repressor and tet operator revealed by new recognition specificities of single amino acid replacement mutants

artículo científico publicado el 20 de agosto de 1992

Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions.

artículo científico publicado en 1983

Control of expression of the Tn10-encoded tetracycline resistance operon II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region ⬇️

artículo científico publicado el 15 de enero de 1984

Correlation of thermodynamic and genetic properties in the Tn10 encoded TET gene control region.

artículo científico publicado en 1982

Coupling physiology and gene regulation in bacteria: the phosphotransferase sugar uptake system delivers the signals

artículo científico publicado en 1998

Cross communication between components of carbon catabolite repression of Lactobacillus casei and Bacillus megaterium.

artículo científico publicado en 2002

Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae

artículo científico publicado en 2003

Crystal structure of the tet repressor in complex with a novel tetracycline, 9-(N,N-dimethylglycylamido)- 6-demethyl-6-deoxy-tetracycline

artículo científico publicado en 1999

Crystallization and preliminary X-ray analyses of catabolite control protein A, free and in complex with its DNA-binding site

artículo científico publicado en 2000

Crystallization of and preliminary X-ray diffraction data for TET-repressor and the TET-repressor-tetracycline complex.

artículo científico publicado en 1984

Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae

artículo científico publicado en 2002

Deletion mutagenesis of Tn 10 Tet repressor--localization of regions important for dimerization and inducibility in vivo.

artículo científico publicado en 1995

Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor ⬇️

artículo científico publicado el 15 de enero de 1998

Determination of the equilibrium association constant between tet repressor and tetracycline at limiting Mg2+ concentrations: A generally applicable method for effector-dependent high-affinity complexes ⬇️

artículo científico publicado el 1 de diciembre de 1991

Development of a transactivator in hepatoma cells that allows expression of phase I, phase II, and chemical defense genes.

artículo científico publicado en 2005

Diarylpropane-1,3-dione derivatives as TetR-inducing tetracycline mimetics: Synthesis and biological investigations

artículo científico publicado en 2009

Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.

artículo científico

Domain motions accompanying Tet repressor induction defined by changes of interspin distances at selectively labeled sites

artículo científico publicado en 1999

Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo

artículo científico publicado en 2003

Dynamics of repressor-operator recognition: the Tn10-encoded tetracycline resistance control.

artículo científico publicado en 1988

Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site

artículo científico publicado en 2007

Electrophoretic analysis of protein-induced DNA bending and twist changes.

artículo científico publicado en 1993

Engineered Tet repressor mutants with single tryptophan residues as fluorescent probes. Solvent accessibilities of DNA and inducer binding sites and interaction with tetracycline

artículo científico

Engineered Tet repressors with recognition specificity for the tetO-4C5G operator variant

artículo científico

Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity

artículo científico publicado en 2000

Expression of alkane hydroxylase from Acinetobacter sp. Strain ADP1 is induced by a broad range of n-alkanes and requires the transcriptional activator AlkR.

artículo científico publicado en 1998

Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.

artículo científico publicado en 1997

Fast large-scale purification of tetracycline repressor variants from overproducing Escherichia coli strains

artículo científico publicado en 1996

Functional roles of amino acid residues involved in forming the alpha-helix-turn-alpha-helix operator DNA binding motif of Tet repressor from Tn10

artículo científico publicado el 1 de octubre de 1992

Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis

artículo científico publicado en 2008

Gene regulation by tetracyclines

artículo científico publicado en 2004

Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes

artículo científico publicado en 2003

Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase

artículo científico publicado en 2006

Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes

artículo científico publicado en 1999

Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250.

artículo científico publicado en 1995

HPr kinase/phosphatase of Bacillus subtilis: expression of the gene and effects of mutations on enzyme activity, growth and carbon catabolite repression

artículo científico publicado en 2002

Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants

artículo científico publicado en 1985

How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor

artículo científico publicado en 2007

How does Mg(2+) affect the binding of anhydrotetracycline in the TetR protein?

artículo científico publicado en 2004

Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1 ⬇️

artículo científico publicado el 1 de julio de 1997

Identification of a stability determinant on the edge of the Tet repressor four-helix bundle dimerization motif

artículo científico publicado en 2001

Improved single-chain transactivators of the Tet-On gene expression system

artículo científico publicado en 2007

In vivo activation of tetracycline repressor by Cre/lox-mediated gene assembly

artículo científico publicado en 2009

In vivo gene silencing identifies the Mycobacterium tuberculosis proteasome as essential for the bacteria to persist in mice

scientific journal article

Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants

artículo científico publicado en 2004

Inducible DNA-loop formation blocks transcriptional activation by an SV40 enhancer

artículo científico publicado en 2005

Induction of single chain tetracycline repressor requires the binding of two inducers

artículo científico publicado en 2006

Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes

artículo científico publicado en 2005

Interference of components of the phosphoenolpyruvate phosphotransferase system with the central virulence gene regulator PrfA of Listeria monocytogenes

artículo científico publicado en 2006

Intragenic Suppressors of Induction-Deficient TetR Mutants: Localization and Potential Mechanism of Action ⬇️

artículo científico publicado el 1 de febrero de 1998

Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by fluorescence measurements. Evidence for divalent metal ion requirement and energy transfer

artículo científico publicado en 1986

Lack of a 5' non-coding region in Tn1721encodedtetRmRNA is associated with a low efficiency of translation and a short half-life inEscherichia coil ⬇️

artículo científico publicado el 11 de septiembre de 1991

Ligand-modulated binding of a gene regulatory protein to DNA. Quantitative analysis of cyclic-AMP induced binding of CRP from Escherichia coli to non-specific and specific DNA targets

scientific article published on 01 June 1989

Mechanism of Tet repressor induction by tetracyclines: length compensates for sequence in the alpha8-alpha9 loop

artículo científico publicado en 2001

Mechanisms underlying expression of Tn10 encoded tetracycline resistance

artículo científico publicado en 1994

Molecular mechanics analysis of Tet repressor TRP-43 fluorescence

artículo científico publicado en 1997

Mycoplasma pneumoniae HPr kinase/phosphorylase

artículo científico publicado en 2004

Nomenclature for new tetracycline resistance determinants

artículo científico publicado en 1999

Nomenclature for tetracycline resistance determinants

artículo científico publicado en 1989

Noninducible Tet repressor mutations map from the operator binding motif to the C terminus

artículo científico publicado en 1993

Nucleotide sequence of a putative periplasmic Mn superoxide dismutase from Acinetobacter calcoaceticus ADP1.

artículo científico publicado en 1997

Nucleotide sequence of the Tn10 encoded tetracycline resistance gene ⬇️

artículo científico publicado el 25 de enero de 1983

Nucleotide sequence of the gene, protein purification and characterization of the pSC101-encoded tetracycline resistance-gene-repressor ⬇️

artículo científico publicado el 1 de noviembre de 1984

Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes ⬇️

artículo científico publicado el 25 de octubre de 1984

Observing conformational and activity changes of tet repressor in vivo.

artículo científico publicado en 2000

Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased

artículo científico publicado en 1994

On the origin of selectivity in recognition by cyclic adenosine 3',5'-monophosphate receptor protein of its specific binding site of the lactose promoter region

artículo científico publicado en 1983

Permeation of tetracyclines through membranes of liposomes and Escherichia coli

scientific article published on 01 January 2000

Phenotypes of combined tet repressor mutants for effector and operator recognition and allostery.

artículo científico publicado en 2004

Phosphoprotein Crh-Ser46-P displays altered binding to CcpA to effect carbon catabolite regulation

scientific article published on 29 November 2005

Pressure effect on the structure of the Tet repressor protein TetR(B).

artículo científico publicado en 2002

Promoter strength driving TetR determines the regulatory properties of Tet-controlled expression systems

artículo científico publicado en 2012

Protein expression can be monitored in yeast by peptide-mediated induction of TetR-controlled gene expression

artículo científico publicado en 2012

Proximity mapping of the Tet repressor-tetracycline-Fe2+ complex by hydrogen peroxide mediated protein cleavage.

artículo científico publicado en 1995

Proximity probing of Tet repressor to tet operator by dimethylsulfate reveals protected and accessible functions for each recognized base-pair in the major groove.

artículo científico publicado en 1995

Quantification of the influence of HPrSer46P on CcpA-cre interaction

artículo científico publicado en 2002

Quantitative analysis of gene expression with an improved green fluorescent protein

article

Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis

artículo científico publicado en 2005

RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system

artículo científico publicado en 1992

Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression

artículo científico publicado en 2006

Regulated and prolonged expression of mIFN(alpha) in immunocompetent mice mediated by a helper-dependent adenovirus vector

artículo científico publicado en 2001

Regulated expression of HPrK/P does not affect carbon catabolite repression of the xyn operon and of rocG in Bacillus subtilis

artículo científico publicado en 2006

Regulation of carbon catabolism in Bacillus species.

artículo científico publicado en 2000

Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange

artículo científico publicado en 2005

RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus

artículo científico publicado en 1994

Saturation mutagenesis of the Tn10-encoded tet operator O1. Identification of base-pairs involved in Tet repressor recognition

scientific article published on 01 August 1988

Short peptides act as inducers, anti-inducers and corepressors of Tet repressor

artículo científico publicado en 2011

Silencing Mycobacterium smegmatis by using tetracycline repressors

artículo científico publicado en 2007

Single-chain Tet transregulators

artículo científico publicado en 2003

Solvent-exposed residues in the Tet repressor (TetR) four-helix bundle contribute to subunit recognition and dimer stability.

artículo científico publicado en 1999

Species-specific differences in the activity of PrfA, the key regulator of listerial virulence genes

artículo científico publicado en 2006

Specific binding of divalent metal ions to tetracycline and to the Tet repressor/tetracycline complex

artículo científico publicado en 2008

Spectroscopic investigation of Tet repressor tryptophan-43 upon specific and nonspecific DNA binding.

artículo científico publicado en 1995

Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity 1 1Edited by R. Ebright ⬇️

artículo científico publicado el 20 de febrero de 1998

Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity 1 1Edited by R. Ebright ⬇️

artículo científico publicado el 20 de febrero de 1998

Stringent doxycycline-dependent control of gene activities using an episomal one-vector system

artículo científico publicado en 2005

Structural Investigation of Tet Repressor Loop 154–167: A Time-Resolved Fluorescence Study of Three Single Trp Mutants

artículo científico publicado en 1997

Structural analysis of the operator binding domain of Tn10-encoded Tet repressor: a time-resolved fluorescence and anisotropy study ⬇️

artículo científico publicado el 25 de febrero de 1992

Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.

artículo científico publicado en 2004

Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system

artículo científico publicado en 2000

Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors glucose 6-phosphate and fructose 1,6-bisphosphate

artículo científico publicado en 2007

Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair

artículo científico publicado en 2009

Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor

artículo científico publicado en 1991

Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance

artículo científico publicado en 1994

Structure-based design of Tet repressor to optimize a new inducer specificity

artículo científico publicado en 2004

Structures of carbon catabolite protein A–(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators ⬇️

artículo científico publicado el 23 de noviembre de 2010

Subtype Selective Tetracycline Agonists and their Application for a Two-Stage Regulatory System

artículo científico publicado en 2006

TET repressor.tet operator complex formation induces conformational changes in the tet operator DNA.

artículo científico publicado en 1984

Teaching TetR to recognize a new inducer

artículo científico publicado en 2003

Tet repressor binding induced curvature of tet operator DNA

artículo científico publicado en 1989

Tet repressor induction without Mg2+

scientific article published on 01 September 2000

Tet repressor mutants with altered effector binding and allostery

artículo científico publicado en 2005

Tet repressor residues indirectly recognizing anhydrotetracycline

artículo científico publicado en 2004

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction

scientific article published on 01 June 1996

Tetracycline derivatives: alternative effectors for Tet transregulators

artículo científico publicado en 2004

Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus

artículo científico publicado en 1995

Tetracycline sensing using novel doxycycline derivatives immobilized on different surface plasmon resonance biosensor surfaces

artículo científico publicado en 2010

Tetracycline-dependent conditional gene knockout in Bacillus subtilis

artículo científico publicado en 2005

Tetracycline-dependent gene regulation: combinations of transregulators yield a variety of expression windows

artículo científico publicado en 2002

Tetracycline-inducible expression systems with reduced basal activity in mammalian cells

artículo científico publicado en 1999

Tetracycline-inducible systems for Drosophila

artículo científico publicado en 2001

Tetracyclines: antibiotic action, uptake, and resistance mechanisms

artículo científico publicado en 1996

The Tetracycline Repressor-A Paradigm for a Biological Switch

artículo científico publicado en 2000

The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region ⬇️

artículo científico publicado el 1 de abril de 1992

The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance

artículo científico publicado en 1995

The p65 domain from NF-kappaB is an efficient human activator in the tetracycline-regulatable gene expression system

artículo científico publicado en 2000

The role of the variable region in Tet repressor for inducibility by tetracycline

artículo científico publicado en 1997

Thermal denaturation of engineered tet repressor proteins and their complexes with tet operator and tetracycline studied by temperature gradient gel electrophoresis.

artículo científico publicado en 1988

Thermodynamic analysis of tetracycline-mediated induction of Tet repressor by a quantitative methylation protection assay

scientific article published on 01 December 1995

Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2(s)-M2 tetracycline transactivator and repressor system.

artículo científico publicado en 2002

Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants.

artículo científico publicado en 2004

Transcriptional activation by tetracyclines in mammalian cells

artículo científico publicado en 1995

Trp scanning analysis of Tet repressor reveals conformational changes associated with operator and anhydrotetracycline binding

artículo científico publicado en 2000

Tryptophan in alpha-helix 3 of Tet repressor forms a sequence-specific contact with tet operator in solution

scientific article published on 01 September 1987

Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1 ⬇️

artículo científico publicado el 1 de junio de 1995

Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor

artículo científico publicado en 2004

UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus

artículo científico publicado en 1994

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